Hybridization methods for DNA sequencing.
Identifieur interne : 000541 ( Ncbi/Checkpoint ); précédent : 000540; suivant : 000542Hybridization methods for DNA sequencing.
Auteurs : W. Bains [Royaume-Uni]Source :
- Genomics [ 0888-7543 ] ; 1991.
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Abstract
I have conducted a general analysis of the practicability of using oligonucleotide hybridization to sequence DNA. Any DNA sequence may be sequenced by hybridization with a complete panel of oligonucleotides. However, sequencing DNA segments over 2 kb long requires an unrealistic number of hybridization reactions. The optimal protocol is to hybridize 7-mer or 8-mer mixed oligonucleotide probes to immobilized DNA fragments 80 bp long: should this prove impractical, hybridization of labeled 270-bp fragments to immobilized mixed 10-mers is a potential alternative. Both protocols require no more experiments to sequence large regions of DNA than conventional m13-based sequencing and are much easier to automate, thus reducing the requirements for skilled personnel. In the ideal case, hybridization sequencing reduces the number of experiments required to sequence megabase DNA by 90%.
DOI: 10.1016/0888-7543(91)90135-2
PubMed: 1769648
Affiliations:
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