Serum IgE reactive against small myelin protein-derived peptides is increased in multiple sclerosis patients.
Identifieur interne : 000470 ( Ncbi/Checkpoint ); précédent : 000469; suivant : 000471Serum IgE reactive against small myelin protein-derived peptides is increased in multiple sclerosis patients.
Auteurs : Daniel D. Mikol [États-Unis] ; Charles Ditlow ; Danielle Usatin ; Pinaki Biswas ; Jack Kalbfleisch ; Andrew Milner ; Emanuel CalenoffSource :
- Journal of neuroimmunology [ 0165-5728 ] ; 2006.
Descripteurs français
- KwdFr :
- Adulte, Adulte d'âge moyen, Auto-immunité (immunologie), Autoanticorps (immunologie), Dosage radioimmunologique (), Femelle, Fragments peptidiques (immunologie), Humains, Immunoglobuline E (analyse), Immunoglobuline E (immunologie), Immunoglobuline E (sang), Mâle, Protéines de la myéline (immunologie), Régulation positive (immunologie), Sclérose en plaques (diagnostic), Sclérose en plaques (immunologie), Sclérose en plaques (sang), Valeur prédictive des tests, Épitopes (immunologie), Études transversales.
- MESH :
- analyse : Immunoglobuline E.
- diagnostic : Sclérose en plaques.
- immunologie : Auto-immunité, Autoanticorps, Fragments peptidiques, Immunoglobuline E, Protéines de la myéline, Régulation positive, Sclérose en plaques, Épitopes.
- sang : Immunoglobuline E, Sclérose en plaques.
- Adulte, Adulte d'âge moyen, Dosage radioimmunologique, Femelle, Humains, Mâle, Valeur prédictive des tests, Études transversales.
English descriptors
- KwdEn :
- Adult, Autoantibodies (immunology), Autoimmunity (immunology), Cross-Sectional Studies, Epitopes (immunology), Female, Humans, Immunoglobulin E (analysis), Immunoglobulin E (blood), Immunoglobulin E (immunology), Male, Middle Aged, Multiple Sclerosis (blood), Multiple Sclerosis (diagnosis), Multiple Sclerosis (immunology), Myelin Proteins (immunology), Peptide Fragments (immunology), Predictive Value of Tests, Radioimmunoassay (methods), Up-Regulation (immunology).
- MESH :
- chemical , analysis : Immunoglobulin E.
- chemical , blood : Immunoglobulin E.
- chemical , immunology : Autoantibodies, Epitopes, Immunoglobulin E, Myelin Proteins, Peptide Fragments.
- blood : Multiple Sclerosis.
- diagnosis : Multiple Sclerosis.
- immunology : Autoimmunity, Multiple Sclerosis, Up-Regulation.
- methods : Radioimmunoassay.
- Adult, Cross-Sectional Studies, Female, Humans, Male, Middle Aged, Predictive Value of Tests.
Abstract
Though independent findings suggest roles for the allergic arm of the immune system and myelin-reactive antibodies in MS, myelin-reactive IgE has not been investigated. We have developed a radioimmunoassay that measures reactive IgE, IgG and IgA against short (5-6-mers) myelin protein-derived peptides bearing little to no sequence identity with other human proteins, and which might therefore be targets of a CNS-specific autoimmune attack. Here we show that, irrespective of clinical subtype, MS patients' sera are characterized by a higher frequency of measurable IgE against the peptides. Moreover, in controls with measurable IgE reactive against test peptides, IgG or IgA reactive with the same peptide epitopes is almost always present in vastly greater quantities, whereas in MS subjects peptide-reactive IgA or IgG is often undetectable. The sensitivity of the full assay, when considering overall positive a serum sample that has detectable autoreactive IgE without other competing Igs, is 69% (S.E.: 5%), with a specificity of 87% (S.E.: 9%). We speculate that IgE reactive against CNS target antigens may have both diagnostic and pathogenic significance, particularly if other peptide-specific, potentially blocking Igs are absent.
DOI: 10.1016/j.jneuroim.2006.06.030
PubMed: 16996143
Affiliations:
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Mikol</name><affiliation wicri:level="1"><nlm:affiliation>Department of Neurology, University of Michigan, Ann Arbor, Michigan 48109, USA. dmiko@umich.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurology, University of Michigan, Ann Arbor, Michigan 48109</wicri:regionArea><wicri:noRegion>Michigan 48109</wicri:noRegion></affiliation></author><author><name sortKey="Ditlow, Charles" sort="Ditlow, Charles" uniqKey="Ditlow C" first="Charles" last="Ditlow">Charles Ditlow</name></author><author><name sortKey="Usatin, Danielle" sort="Usatin, Danielle" uniqKey="Usatin D" first="Danielle" last="Usatin">Danielle Usatin</name></author><author><name sortKey="Biswas, Pinaki" sort="Biswas, Pinaki" uniqKey="Biswas P" first="Pinaki" last="Biswas">Pinaki Biswas</name></author><author><name sortKey="Kalbfleisch, Jack" sort="Kalbfleisch, Jack" uniqKey="Kalbfleisch J" first="Jack" last="Kalbfleisch">Jack Kalbfleisch</name></author><author><name sortKey="Milner, Andrew" sort="Milner, Andrew" uniqKey="Milner A" first="Andrew" last="Milner">Andrew Milner</name></author><author><name sortKey="Calenoff, Emanuel" sort="Calenoff, Emanuel" uniqKey="Calenoff E" first="Emanuel" last="Calenoff">Emanuel Calenoff</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16996143</idno><idno type="pmid">16996143</idno><idno type="doi">10.1016/j.jneuroim.2006.06.030</idno><idno type="wicri:Area/PubMed/Corpus">002223</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002223</idno><idno type="wicri:Area/PubMed/Curation">002223</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002223</idno><idno type="wicri:Area/PubMed/Checkpoint">002092</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002092</idno><idno type="wicri:Area/Ncbi/Merge">000470</idno><idno type="wicri:Area/Ncbi/Curation">000470</idno><idno type="wicri:Area/Ncbi/Checkpoint">000470</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Serum IgE reactive against small myelin protein-derived peptides is increased in multiple sclerosis patients.</title><author><name sortKey="Mikol, Daniel D" sort="Mikol, Daniel D" uniqKey="Mikol D" first="Daniel D" last="Mikol">Daniel D. Mikol</name><affiliation wicri:level="1"><nlm:affiliation>Department of Neurology, University of Michigan, Ann Arbor, Michigan 48109, USA. dmiko@umich.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Neurology, University of Michigan, Ann Arbor, Michigan 48109</wicri:regionArea><wicri:noRegion>Michigan 48109</wicri:noRegion></affiliation></author><author><name sortKey="Ditlow, Charles" sort="Ditlow, Charles" uniqKey="Ditlow C" first="Charles" last="Ditlow">Charles Ditlow</name></author><author><name sortKey="Usatin, Danielle" sort="Usatin, Danielle" uniqKey="Usatin D" first="Danielle" last="Usatin">Danielle Usatin</name></author><author><name sortKey="Biswas, Pinaki" sort="Biswas, Pinaki" uniqKey="Biswas P" first="Pinaki" last="Biswas">Pinaki Biswas</name></author><author><name sortKey="Kalbfleisch, Jack" sort="Kalbfleisch, Jack" uniqKey="Kalbfleisch J" first="Jack" last="Kalbfleisch">Jack Kalbfleisch</name></author><author><name sortKey="Milner, Andrew" sort="Milner, Andrew" uniqKey="Milner A" first="Andrew" last="Milner">Andrew Milner</name></author><author><name sortKey="Calenoff, Emanuel" sort="Calenoff, Emanuel" uniqKey="Calenoff E" first="Emanuel" last="Calenoff">Emanuel Calenoff</name></author></analytic><series><title level="j">Journal of neuroimmunology</title><idno type="ISSN">0165-5728</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Autoantibodies (immunology)</term><term>Autoimmunity (immunology)</term><term>Cross-Sectional Studies</term><term>Epitopes (immunology)</term><term>Female</term><term>Humans</term><term>Immunoglobulin E (analysis)</term><term>Immunoglobulin E (blood)</term><term>Immunoglobulin E (immunology)</term><term>Male</term><term>Middle Aged</term><term>Multiple Sclerosis (blood)</term><term>Multiple Sclerosis (diagnosis)</term><term>Multiple Sclerosis (immunology)</term><term>Myelin Proteins (immunology)</term><term>Peptide Fragments (immunology)</term><term>Predictive Value of Tests</term><term>Radioimmunoassay (methods)</term><term>Up-Regulation (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Auto-immunité (immunologie)</term><term>Autoanticorps (immunologie)</term><term>Dosage radioimmunologique ()</term><term>Femelle</term><term>Fragments peptidiques (immunologie)</term><term>Humains</term><term>Immunoglobuline E (analyse)</term><term>Immunoglobuline E (immunologie)</term><term>Immunoglobuline E (sang)</term><term>Mâle</term><term>Protéines de la myéline (immunologie)</term><term>Régulation positive (immunologie)</term><term>Sclérose en plaques (diagnostic)</term><term>Sclérose en plaques (immunologie)</term><term>Sclérose en plaques (sang)</term><term>Valeur prédictive des tests</term><term>Épitopes (immunologie)</term><term>Études transversales</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Immunoglobulin E</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Autoantibodies</term><term>Epitopes</term><term>Immunoglobulin E</term><term>Myelin Proteins</term><term>Peptide Fragments</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Immunoglobuline E</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Multiple Sclerosis</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Multiple Sclerosis</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Sclérose en plaques</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Auto-immunité</term><term>Autoanticorps</term><term>Fragments peptidiques</term><term>Immunoglobuline E</term><term>Protéines de la myéline</term><term>Régulation positive</term><term>Sclérose en plaques</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Autoimmunity</term><term>Multiple Sclerosis</term><term>Up-Regulation</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Radioimmunoassay</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Immunoglobuline E</term><term>Sclérose en plaques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Cross-Sectional Studies</term><term>Female</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Predictive Value of Tests</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Adulte d'âge moyen</term><term>Dosage 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We have developed a radioimmunoassay that measures reactive IgE, IgG and IgA against short (5-6-mers) myelin protein-derived peptides bearing little to no sequence identity with other human proteins, and which might therefore be targets of a CNS-specific autoimmune attack. Here we show that, irrespective of clinical subtype, MS patients' sera are characterized by a higher frequency of measurable IgE against the peptides. Moreover, in controls with measurable IgE reactive against test peptides, IgG or IgA reactive with the same peptide epitopes is almost always present in vastly greater quantities, whereas in MS subjects peptide-reactive IgA or IgG is often undetectable. The sensitivity of the full assay, when considering overall positive a serum sample that has detectable autoreactive IgE without other competing Igs, is 69% (S.E.: 5%), with a specificity of 87% (S.E.: 9%). We speculate that IgE reactive against CNS target antigens may have both diagnostic and pathogenic significance, particularly if other peptide-specific, potentially blocking Igs are absent.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Biswas, Pinaki" sort="Biswas, Pinaki" uniqKey="Biswas P" first="Pinaki" last="Biswas">Pinaki Biswas</name><name sortKey="Calenoff, Emanuel" sort="Calenoff, Emanuel" uniqKey="Calenoff E" first="Emanuel" last="Calenoff">Emanuel Calenoff</name><name sortKey="Ditlow, Charles" sort="Ditlow, Charles" uniqKey="Ditlow C" first="Charles" last="Ditlow">Charles Ditlow</name><name sortKey="Kalbfleisch, Jack" sort="Kalbfleisch, Jack" uniqKey="Kalbfleisch J" first="Jack" last="Kalbfleisch">Jack Kalbfleisch</name><name sortKey="Milner, Andrew" sort="Milner, Andrew" uniqKey="Milner A" first="Andrew" last="Milner">Andrew Milner</name><name sortKey="Usatin, Danielle" sort="Usatin, Danielle" uniqKey="Usatin D" first="Danielle" last="Usatin">Danielle Usatin</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Mikol, Daniel D" sort="Mikol, Daniel D" uniqKey="Mikol D" first="Daniel D" last="Mikol">Daniel D. Mikol</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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