Selection of CC chemokine receptor 5-binding peptide from a phage display peptide library.
Identifieur interne : 000468 ( Ncbi/Checkpoint ); précédent : 000467; suivant : 000469Selection of CC chemokine receptor 5-binding peptide from a phage display peptide library.
Auteurs : Fang-Yu Wang [République populaire de Chine] ; Tian-Yuan Zhang ; Jin-Xian Luo ; Guo-An He ; Qu-Liang Gu ; Fan XiaoSource :
- Bioscience, biotechnology, and biochemistry [ 0916-8451 ] ; 2006.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Antagonistes des récepteurs CCR5, Anticorps monoclonaux (métabolisme), Banque de peptides, Cellules CHO, Chimiokine CCL5 (métabolisme), Cricetinae, Fixation compétitive, Humains, Liaison aux protéines, Motifs d'acides aminés, Récepteurs CCR5 (génétique), Récepteurs CCR5 (métabolisme), Signalisation du calcium, Séquence d'acides aminés, Séquence nucléotidique, Transfection.
- MESH :
- génétique : Récepteurs CCR5.
- métabolisme : Anticorps monoclonaux, Chimiokine CCL5, Récepteurs CCR5.
- Analyse de séquence d'ADN, Animaux, Antagonistes des récepteurs CCR5, Banque de peptides, Cellules CHO, Cricetinae, Fixation compétitive, Humains, Liaison aux protéines, Motifs d'acides aminés, Signalisation du calcium, Séquence d'acides aminés, Séquence nucléotidique, Transfection.
English descriptors
- KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Animals, Antibodies, Monoclonal (metabolism), Base Sequence, Binding, Competitive, CCR5 Receptor Antagonists, CHO Cells, Calcium Signaling, Chemokine CCL5 (metabolism), Cricetinae, Humans, Peptide Library, Protein Binding, Receptors, CCR5 (genetics), Receptors, CCR5 (metabolism), Sequence Analysis, DNA, Transfection.
- MESH :
- chemical , genetics : Receptors, CCR5.
- chemical , metabolism : Antibodies, Monoclonal, Chemokine CCL5, Receptors, CCR5.
- Amino Acid Motifs, Amino Acid Sequence, Animals, Base Sequence, Binding, Competitive, CCR5 Receptor Antagonists, CHO Cells, Calcium Signaling, Cricetinae, Humans, Peptide Library, Protein Binding, Sequence Analysis, DNA, Transfection.
Abstract
Human CC chemokine receptor (CCR) 5 is a G protein-coupled receptor involved in a broad range of human diseases that mediates HIV-1 viral entry into cells. Certain small molecule receptor antagonists to CCR5 have been useful in therapy for these diseases. In this study, CCR5-expressing CHO cells (CHO/CCR5 cells) were used to select CCR5-binding peptides from a phage-displayed 12-mers peptide library. All of the 30 clones selected from the library showed specific binding to CHO/CCR5 cells by enzyme linked immunosorbent assay (ELISA). Seventeen out of the 30 clones shared the amino acid motif AFDWTFVPSLIL. The motif-containing phages and synthetic peptide AFDWTFVPSLIL blocked the binding of mAb 2D7 to CHO/CCR5 cells and competitively inhibited the ability of chemokine regulated on activation normal T cell expressed and secreted (RANTES) binding to CHO/CCR5 cells. Furthermore, the peptide AFDWTFVPSLIL also inhibited RANTES induced increase in the intracellular Ca2+ level in CHO/CCR5 cells. These results suggest that the peptide AFDWTFVPSLIL was specific for CCR5 and that it might become a CCR5 antagonist.
DOI: 10.1271/bbb.50654
PubMed: 16960395
Affiliations:
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pubmed:16960395Le document en format XML
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sort="Luo, Jin Xian" uniqKey="Luo J" first="Jin-Xian" last="Luo">Jin-Xian Luo</name></author><author><name sortKey="He, Guo An" sort="He, Guo An" uniqKey="He G" first="Guo-An" last="He">Guo-An He</name></author><author><name sortKey="Gu, Qu Liang" sort="Gu, Qu Liang" uniqKey="Gu Q" first="Qu-Liang" last="Gu">Qu-Liang Gu</name></author><author><name sortKey="Xiao, Fan" sort="Xiao, Fan" uniqKey="Xiao F" first="Fan" last="Xiao">Fan Xiao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16960395</idno><idno type="pmid">16960395</idno><idno type="doi">10.1271/bbb.50654</idno><idno type="wicri:Area/PubMed/Corpus">002224</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002224</idno><idno type="wicri:Area/PubMed/Curation">002224</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002224</idno><idno 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Yat-sen University, Guanzhou</wicri:regionArea><wicri:noRegion>Guanzhou</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Tian Yuan" sort="Zhang, Tian Yuan" uniqKey="Zhang T" first="Tian-Yuan" last="Zhang">Tian-Yuan Zhang</name></author><author><name sortKey="Luo, Jin Xian" sort="Luo, Jin Xian" uniqKey="Luo J" first="Jin-Xian" last="Luo">Jin-Xian Luo</name></author><author><name sortKey="He, Guo An" sort="He, Guo An" uniqKey="He G" first="Guo-An" last="He">Guo-An He</name></author><author><name sortKey="Gu, Qu Liang" sort="Gu, Qu Liang" uniqKey="Gu Q" first="Qu-Liang" last="Gu">Qu-Liang Gu</name></author><author><name sortKey="Xiao, Fan" sort="Xiao, Fan" uniqKey="Xiao F" first="Fan" last="Xiao">Fan Xiao</name></author></analytic><series><title level="j">Bioscience, biotechnology, and biochemistry</title><idno type="ISSN">0916-8451</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal (metabolism)</term><term>Base Sequence</term><term>Binding, Competitive</term><term>CCR5 Receptor Antagonists</term><term>CHO Cells</term><term>Calcium Signaling</term><term>Chemokine CCL5 (metabolism)</term><term>Cricetinae</term><term>Humans</term><term>Peptide Library</term><term>Protein Binding</term><term>Receptors, CCR5 (genetics)</term><term>Receptors, CCR5 (metabolism)</term><term>Sequence Analysis, DNA</term><term>Transfection</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Antagonistes des récepteurs CCR5</term><term>Anticorps monoclonaux (métabolisme)</term><term>Banque de peptides</term><term>Cellules CHO</term><term>Chimiokine CCL5 (métabolisme)</term><term>Cricetinae</term><term>Fixation compétitive</term><term>Humains</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Récepteurs CCR5 (génétique)</term><term>Récepteurs CCR5 (métabolisme)</term><term>Signalisation du calcium</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, CCR5</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Chemokine CCL5</term><term>Receptors, CCR5</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Récepteurs CCR5</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps monoclonaux</term><term>Chimiokine CCL5</term><term>Récepteurs CCR5</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Animals</term><term>Base Sequence</term><term>Binding, Competitive</term><term>CCR5 Receptor Antagonists</term><term>CHO Cells</term><term>Calcium Signaling</term><term>Cricetinae</term><term>Humans</term><term>Peptide Library</term><term>Protein Binding</term><term>Sequence Analysis, DNA</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Antagonistes des récepteurs CCR5</term><term>Banque de peptides</term><term>Cellules CHO</term><term>Cricetinae</term><term>Fixation compétitive</term><term>Humains</term><term>Liaison aux protéines</term><term>Motifs d'acides aminés</term><term>Signalisation du calcium</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Transfection</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Human CC chemokine receptor (CCR) 5 is a G protein-coupled receptor involved in a broad range of human diseases that mediates HIV-1 viral entry into cells. Certain small molecule receptor antagonists to CCR5 have been useful in therapy for these diseases. In this study, CCR5-expressing CHO cells (CHO/CCR5 cells) were used to select CCR5-binding peptides from a phage-displayed 12-mers peptide library. All of the 30 clones selected from the library showed specific binding to CHO/CCR5 cells by enzyme linked immunosorbent assay (ELISA). Seventeen out of the 30 clones shared the amino acid motif AFDWTFVPSLIL. The motif-containing phages and synthetic peptide AFDWTFVPSLIL blocked the binding of mAb 2D7 to CHO/CCR5 cells and competitively inhibited the ability of chemokine regulated on activation normal T cell expressed and secreted (RANTES) binding to CHO/CCR5 cells. Furthermore, the peptide AFDWTFVPSLIL also inhibited RANTES induced increase in the intracellular Ca2+ level in CHO/CCR5 cells. These results suggest that the peptide AFDWTFVPSLIL was specific for CCR5 and that it might become a CCR5 antagonist.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Gu, Qu Liang" sort="Gu, Qu Liang" uniqKey="Gu Q" first="Qu-Liang" last="Gu">Qu-Liang Gu</name><name sortKey="He, Guo An" sort="He, Guo An" uniqKey="He G" first="Guo-An" last="He">Guo-An He</name><name sortKey="Luo, Jin Xian" sort="Luo, Jin Xian" uniqKey="Luo J" first="Jin-Xian" last="Luo">Jin-Xian Luo</name><name sortKey="Xiao, Fan" sort="Xiao, Fan" uniqKey="Xiao F" first="Fan" last="Xiao">Fan Xiao</name><name sortKey="Zhang, Tian Yuan" sort="Zhang, Tian Yuan" uniqKey="Zhang T" first="Tian-Yuan" last="Zhang">Tian-Yuan Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Wang, Fang Yu" sort="Wang, Fang Yu" uniqKey="Wang F" first="Fang-Yu" last="Wang">Fang-Yu Wang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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