Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs.
Identifieur interne : 000318 ( Ncbi/Checkpoint ); précédent : 000317; suivant : 000319Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs.
Auteurs : E. Wickstrom [États-Unis] ; M. Choob ; K A Urtishak ; X. Tian ; N. Sternheim ; S. Talbot ; J. Archdeacon ; V A Efimov ; S A FarberSource :
- Journal of drug targeting [ 1061-186X ] ; 2004.
Descripteurs français
- KwdFr :
- ARN messager (antagonistes et inhibiteurs), ARN messager (biosynthèse), Acides nucléiques peptidiques (), Animaux, Danio zébré (embryologie), Embryon non mammalien (), Embryon non mammalien (métabolisme), Hydroxyproline (), Mimétisme moléculaire, Oligoribonucléotides antisens (), Oligoribonucléotides antisens (pharmacologie), Phosphonates (), Séquence nucléotidique.
- MESH :
- antagonistes et inhibiteurs : ARN messager.
- biosynthèse : ARN messager.
- embryologie : Danio zébré.
- métabolisme : Embryon non mammalien.
- pharmacologie : Oligoribonucléotides antisens.
- Acides nucléiques peptidiques, Animaux, Embryon non mammalien, Hydroxyproline, Mimétisme moléculaire, Oligoribonucléotides antisens, Phosphonates, Séquence nucléotidique.
English descriptors
- KwdEn :
- Animals, Base Sequence, Embryo, Nonmammalian (drug effects), Embryo, Nonmammalian (metabolism), Hydroxyproline (chemistry), Molecular Mimicry, Oligoribonucleotides, Antisense (chemistry), Oligoribonucleotides, Antisense (pharmacology), Organophosphonates (chemistry), Peptide Nucleic Acids (chemistry), RNA, Messenger (antagonists & inhibitors), RNA, Messenger (biosynthesis), Zebrafish (embryology).
- MESH :
- chemical , antagonists & inhibitors : RNA, Messenger.
- chemical , biosynthesis : RNA, Messenger.
- chemical , chemistry : Hydroxyproline, Oligoribonucleotides, Antisense, Organophosphonates, Peptide Nucleic Acids.
- drug effects : Embryo, Nonmammalian.
- embryology : Zebrafish.
- metabolism : Embryo, Nonmammalian.
- chemical , pharmacology : Oligoribonucleotides, Antisense.
- Animals, Base Sequence, Molecular Mimicry.
Abstract
Morpholino phosphorodiamidate (MO) DNA mimics display excellent water solubility and hybridization properties toward DNA and RNA, and have been utilized in the model vertebrate zebrafish (Danio rerio) for genome-wide, sequence-based, reverse genetic screens during embryonic development. Peptide nucleic acids (PNAs) exhibit excellent mismatch discrimination, nuclease resistance, and protease resistance, but low solubility. Negatively charged DNA mimics composed of alternating residues of trans-4-hydroxy-L-proline peptide nucleic acid monomers and phosphono peptide nucleic acid monomers (HypNA-pPNA) combine all of the positive features of both MOs and PNAs. Thus, we evaluated PNA oligomers and HypNA-pPNA oligomers as an alternative to MOs for oligonucleotide inhibition of gene expression in zebrafish embryos. We observed that HypNA-pPNA 18-mers displayed comparable potency to MO 25-mers as knockdown agents against chordin, notail and uroD, with greater mismatch stringency. Furthermore, we observed that a specific HypNA-pPNA 18-mer elicited the dharma (bozozok)(-/-) phenotype in zebrafish embryos, which MO 25-mers do not. These observations validate HypNA-pPNAs as an alternative to MO oligomers for reverse genetic studies. The stronger hybridization and greater specificity of HypNA-pPNAs enable knockdown of mRNAs unaffected by MO oligomers.
DOI: 10.1080/10611860412331285242
PubMed: 15545086
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002358
- to stream PubMed, to step Curation: 002358
- to stream PubMed, to step Checkpoint: 002237
- to stream Ncbi, to step Merge: 000318
- to stream Ncbi, to step Curation: 000318
Links to Exploration step
pubmed:15545086Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs.</title><author><name sortKey="Wickstrom, E" sort="Wickstrom, E" uniqKey="Wickstrom E" first="E" last="Wickstrom">E. Wickstrom</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA. eric@tesla.jci.tju.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Choob, M" sort="Choob, M" uniqKey="Choob M" first="M" last="Choob">M. Choob</name></author><author><name sortKey="Urtishak, K A" sort="Urtishak, K A" uniqKey="Urtishak K" first="K A" last="Urtishak">K A Urtishak</name></author><author><name sortKey="Tian, X" sort="Tian, X" uniqKey="Tian X" first="X" last="Tian">X. Tian</name></author><author><name sortKey="Sternheim, N" sort="Sternheim, N" uniqKey="Sternheim N" first="N" last="Sternheim">N. Sternheim</name></author><author><name sortKey="Talbot, S" sort="Talbot, S" uniqKey="Talbot S" first="S" last="Talbot">S. Talbot</name></author><author><name sortKey="Archdeacon, J" sort="Archdeacon, J" uniqKey="Archdeacon J" first="J" last="Archdeacon">J. Archdeacon</name></author><author><name sortKey="Efimov, V A" sort="Efimov, V A" uniqKey="Efimov V" first="V A" last="Efimov">V A Efimov</name></author><author><name sortKey="Farber, S A" sort="Farber, S A" uniqKey="Farber S" first="S A" last="Farber">S A Farber</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15545086</idno><idno type="pmid">15545086</idno><idno type="doi">10.1080/10611860412331285242</idno><idno type="wicri:Area/PubMed/Corpus">002358</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002358</idno><idno type="wicri:Area/PubMed/Curation">002358</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002358</idno><idno type="wicri:Area/PubMed/Checkpoint">002237</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002237</idno><idno type="wicri:Area/Ncbi/Merge">000318</idno><idno type="wicri:Area/Ncbi/Curation">000318</idno><idno type="wicri:Area/Ncbi/Checkpoint">000318</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs.</title><author><name sortKey="Wickstrom, E" sort="Wickstrom, E" uniqKey="Wickstrom E" first="E" last="Wickstrom">E. Wickstrom</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107, USA. eric@tesla.jci.tju.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biochemistry and Molecular Pharmacology, Thomas Jefferson University, Philadelphia, PA 19107</wicri:regionArea><placeName><region type="state">Pennsylvanie</region></placeName></affiliation></author><author><name sortKey="Choob, M" sort="Choob, M" uniqKey="Choob M" first="M" last="Choob">M. Choob</name></author><author><name sortKey="Urtishak, K A" sort="Urtishak, K A" uniqKey="Urtishak K" first="K A" last="Urtishak">K A Urtishak</name></author><author><name sortKey="Tian, X" sort="Tian, X" uniqKey="Tian X" first="X" last="Tian">X. Tian</name></author><author><name sortKey="Sternheim, N" sort="Sternheim, N" uniqKey="Sternheim N" first="N" last="Sternheim">N. Sternheim</name></author><author><name sortKey="Talbot, S" sort="Talbot, S" uniqKey="Talbot S" first="S" last="Talbot">S. Talbot</name></author><author><name sortKey="Archdeacon, J" sort="Archdeacon, J" uniqKey="Archdeacon J" first="J" last="Archdeacon">J. Archdeacon</name></author><author><name sortKey="Efimov, V A" sort="Efimov, V A" uniqKey="Efimov V" first="V A" last="Efimov">V A Efimov</name></author><author><name sortKey="Farber, S A" sort="Farber, S A" uniqKey="Farber S" first="S A" last="Farber">S A Farber</name></author></analytic><series><title level="j">Journal of drug targeting</title><idno type="ISSN">1061-186X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Embryo, Nonmammalian (drug effects)</term><term>Embryo, Nonmammalian (metabolism)</term><term>Hydroxyproline (chemistry)</term><term>Molecular Mimicry</term><term>Oligoribonucleotides, Antisense (chemistry)</term><term>Oligoribonucleotides, Antisense (pharmacology)</term><term>Organophosphonates (chemistry)</term><term>Peptide Nucleic Acids (chemistry)</term><term>RNA, Messenger (antagonists & inhibitors)</term><term>RNA, Messenger (biosynthesis)</term><term>Zebrafish (embryology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (antagonistes et inhibiteurs)</term><term>ARN messager (biosynthèse)</term><term>Acides nucléiques peptidiques ()</term><term>Animaux</term><term>Danio zébré (embryologie)</term><term>Embryon non mammalien ()</term><term>Embryon non mammalien (métabolisme)</term><term>Hydroxyproline ()</term><term>Mimétisme moléculaire</term><term>Oligoribonucléotides antisens ()</term><term>Oligoribonucléotides antisens (pharmacologie)</term><term>Phosphonates ()</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hydroxyproline</term><term>Oligoribonucleotides, Antisense</term><term>Organophosphonates</term><term>Peptide Nucleic Acids</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Embryo, Nonmammalian</term></keywords><keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Danio zébré</term></keywords><keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Zebrafish</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Embryo, Nonmammalian</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Embryon non mammalien</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Oligoribonucléotides antisens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Oligoribonucleotides, Antisense</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Molecular Mimicry</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acides nucléiques peptidiques</term><term>Animaux</term><term>Embryon non mammalien</term><term>Hydroxyproline</term><term>Mimétisme moléculaire</term><term>Oligoribonucléotides antisens</term><term>Phosphonates</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Morpholino phosphorodiamidate (MO) DNA mimics display excellent water solubility and hybridization properties toward DNA and RNA, and have been utilized in the model vertebrate zebrafish (Danio rerio) for genome-wide, sequence-based, reverse genetic screens during embryonic development. Peptide nucleic acids (PNAs) exhibit excellent mismatch discrimination, nuclease resistance, and protease resistance, but low solubility. Negatively charged DNA mimics composed of alternating residues of trans-4-hydroxy-L-proline peptide nucleic acid monomers and phosphono peptide nucleic acid monomers (HypNA-pPNA) combine all of the positive features of both MOs and PNAs. Thus, we evaluated PNA oligomers and HypNA-pPNA oligomers as an alternative to MOs for oligonucleotide inhibition of gene expression in zebrafish embryos. We observed that HypNA-pPNA 18-mers displayed comparable potency to MO 25-mers as knockdown agents against chordin, notail and uroD, with greater mismatch stringency. Furthermore, we observed that a specific HypNA-pPNA 18-mer elicited the dharma (bozozok)(-/-) phenotype in zebrafish embryos, which MO 25-mers do not. These observations validate HypNA-pPNAs as an alternative to MO oligomers for reverse genetic studies. The stronger hybridization and greater specificity of HypNA-pPNAs enable knockdown of mRNAs unaffected by MO oligomers.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Pennsylvanie</li></region></list><tree><noCountry><name sortKey="Archdeacon, J" sort="Archdeacon, J" uniqKey="Archdeacon J" first="J" last="Archdeacon">J. Archdeacon</name><name sortKey="Choob, M" sort="Choob, M" uniqKey="Choob M" first="M" last="Choob">M. Choob</name><name sortKey="Efimov, V A" sort="Efimov, V A" uniqKey="Efimov V" first="V A" last="Efimov">V A Efimov</name><name sortKey="Farber, S A" sort="Farber, S A" uniqKey="Farber S" first="S A" last="Farber">S A Farber</name><name sortKey="Sternheim, N" sort="Sternheim, N" uniqKey="Sternheim N" first="N" last="Sternheim">N. Sternheim</name><name sortKey="Talbot, S" sort="Talbot, S" uniqKey="Talbot S" first="S" last="Talbot">S. Talbot</name><name sortKey="Tian, X" sort="Tian, X" uniqKey="Tian X" first="X" last="Tian">X. Tian</name><name sortKey="Urtishak, K A" sort="Urtishak, K A" uniqKey="Urtishak K" first="K A" last="Urtishak">K A Urtishak</name></noCountry><country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Wickstrom, E" sort="Wickstrom, E" uniqKey="Wickstrom E" first="E" last="Wickstrom">E. Wickstrom</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000318 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000318 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:15545086
|texte= Sequence specificity of alternating hydroyprolyl/phosphono peptide nucleic acids against zebrafish embryo mRNAs.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:15545086" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |