Structure determination of a lone alpha-helical antifreeze protein from winter flounder.
Identifieur interne : 000298 ( Ncbi/Checkpoint ); précédent : 000297; suivant : 000299Structure determination of a lone alpha-helical antifreeze protein from winter flounder.
Auteurs : F. Sicheri [Canada] ; D S YangSource :
- Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 1996.
Abstract
The X-ray crystal structure of a lone alpha-helical antifreeze protein from winter flounder has been determined to 1.5 A using a combination of molecular-replacement and isomorphous-replacement techniques. Molecular replacement involved a multiparameter search using X-PLOR with two 37-mers of alanine in idealized alpha-helical conformations as the search models. Identified were a large number of potential solutions from which the correct solution was not distinguishable. Commitment of the top 1620 solutions to cycles of rigid-body, positional and simulated-annealing refinement identified the correct solution by a small margin in R factor. Low-resolution electron-density maps generated with phasing information from TbNO(3) and LaNO(3) derivatives were consistent with the top molecular-replacement solution. These derivatives also provided a means to filter and compare the large number of other molecular-replacement solutions with reasonable R factor statistics. The structure-solution strategy described herein may prove useful for the determination of other relatively simple alpha-helical X-ray structures.
DOI: 10.1107/S0907444995015253
PubMed: 15299670
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002775
- to stream PubMed, to step Curation: 002775
- to stream PubMed, to step Checkpoint: 002606
- to stream Ncbi, to step Merge: 000298
- to stream Ncbi, to step Curation: 000298
Links to Exploration step
pubmed:15299670Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Structure determination of a lone alpha-helical antifreeze protein from winter flounder.</title><author><name sortKey="Sicheri, F" sort="Sicheri, F" uniqKey="Sicheri F" first="F" last="Sicheri">F. Sicheri</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, Faculty of Health Science, McMaster University, Hamilton, Ontario, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Faculty of Health Science, McMaster University, Hamilton, Ontario</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Yang, D S" sort="Yang, D S" uniqKey="Yang D" first="D S" last="Yang">D S Yang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:15299670</idno><idno type="pmid">15299670</idno><idno type="doi">10.1107/S0907444995015253</idno><idno type="wicri:Area/PubMed/Corpus">002775</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002775</idno><idno type="wicri:Area/PubMed/Curation">002775</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002775</idno><idno type="wicri:Area/PubMed/Checkpoint">002606</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002606</idno><idno type="wicri:Area/Ncbi/Merge">000298</idno><idno type="wicri:Area/Ncbi/Curation">000298</idno><idno type="wicri:Area/Ncbi/Checkpoint">000298</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Structure determination of a lone alpha-helical antifreeze protein from winter flounder.</title><author><name sortKey="Sicheri, F" sort="Sicheri, F" uniqKey="Sicheri F" first="F" last="Sicheri">F. Sicheri</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry, Faculty of Health Science, McMaster University, Hamilton, Ontario, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biochemistry, Faculty of Health Science, McMaster University, Hamilton, Ontario</wicri:regionArea><orgName type="university">Université McMaster</orgName><placeName><settlement type="city">Hamilton (Ontario)</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Yang, D S" sort="Yang, D S" uniqKey="Yang D" first="D S" last="Yang">D S Yang</name></author></analytic><series><title level="j">Acta crystallographica. Section D, Biological crystallography</title><idno type="ISSN">0907-4449</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The X-ray crystal structure of a lone alpha-helical antifreeze protein from winter flounder has been determined to 1.5 A using a combination of molecular-replacement and isomorphous-replacement techniques. Molecular replacement involved a multiparameter search using X-PLOR with two 37-mers of alanine in idealized alpha-helical conformations as the search models. Identified were a large number of potential solutions from which the correct solution was not distinguishable. Commitment of the top 1620 solutions to cycles of rigid-body, positional and simulated-annealing refinement identified the correct solution by a small margin in R factor. Low-resolution electron-density maps generated with phasing information from TbNO(3) and LaNO(3) derivatives were consistent with the top molecular-replacement solution. These derivatives also provided a means to filter and compare the large number of other molecular-replacement solutions with reasonable R factor statistics. The structure-solution strategy described herein may prove useful for the determination of other relatively simple alpha-helical X-ray structures.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Hamilton (Ontario)</li></settlement><orgName><li>Université McMaster</li></orgName></list><tree><noCountry><name sortKey="Yang, D S" sort="Yang, D S" uniqKey="Yang D" first="D S" last="Yang">D S Yang</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Sicheri, F" sort="Sicheri, F" uniqKey="Sicheri F" first="F" last="Sicheri">F. Sicheri</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000298 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000298 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:15299670
|texte= Structure determination of a lone alpha-helical antifreeze protein from winter flounder.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:15299670" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |