A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Identifieur interne : 000273 ( Ncbi/Checkpoint ); précédent : 000272; suivant : 000274A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Auteurs : Lihua Zhou [République populaire de Chine] ; Huimin Deng ; Qinying Deng ; Shankai ZhaoSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN (analyse), ADN (génétique), Acides picoliniques, Données de séquences moléculaires, Oligodésoxyribonucléotides (analyse), Oligodésoxyribonucléotides (génétique), Pyrazinamide (analogues et dérivés), Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI (instrumentation), Séquence nucléotidique.
- MESH :
- analogues et dérivés : Pyrazinamide.
- analyse : ADN, Oligodésoxyribonucléotides.
- génétique : ADN, Oligodésoxyribonucléotides.
- instrumentation : Acides picoliniques, Données de séquences moléculaires, Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, DNA (analysis), DNA (genetics), Molecular Sequence Data, Oligodeoxyribonucleotides (analysis), Oligodeoxyribonucleotides (genetics), Picolinic Acids, Pyrazinamide (analogs & derivatives), Reproducibility of Results, Solutions, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation).
- MESH :
- chemical , analogs & derivatives : Pyrazinamide.
- chemical , analysis : DNA, Oligodeoxyribonucleotides.
- chemical , genetics : DNA, Oligodeoxyribonucleotides.
- instrumentation : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Base Sequence, Molecular Sequence Data, Picolinic Acids, Reproducibility of Results, Solutions.
Abstract
A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.
DOI: 10.1002/rcm.1404
PubMed: 15052561
Affiliations:
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pubmed:15052561Le document en format XML
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<affiliation wicri:level="1"><nlm:affiliation>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong, P.R. China.</nlm:affiliation>
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<term>Oligodeoxyribonucleotides (genetics)</term>
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<term>Pyrazinamide (analogs & derivatives)</term>
<term>Reproducibility of Results</term>
<term>Solutions</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation)</term>
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<term>Oligodésoxyribonucléotides (génétique)</term>
<term>Pyrazinamide (analogues et dérivés)</term>
<term>Reproductibilité des résultats</term>
<term>Solutions</term>
<term>Spectrométrie de masse MALDI (instrumentation)</term>
<term>Séquence nucléotidique</term>
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<term>Solutions</term>
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<term>Données de séquences moléculaires</term>
<term>Reproductibilité des résultats</term>
<term>Solutions</term>
<term>Spectrométrie de masse MALDI</term>
<term>Séquence nucléotidique</term>
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<front><div type="abstract" xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.</div>
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<name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name>
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