A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Identifieur interne : 000273 ( Ncbi/Checkpoint ); précédent : 000272; suivant : 000274A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Auteurs : Lihua Zhou [République populaire de Chine] ; Huimin Deng ; Qinying Deng ; Shankai ZhaoSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN (analyse), ADN (génétique), Acides picoliniques, Données de séquences moléculaires, Oligodésoxyribonucléotides (analyse), Oligodésoxyribonucléotides (génétique), Pyrazinamide (analogues et dérivés), Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI (instrumentation), Séquence nucléotidique.
- MESH :
- analogues et dérivés : Pyrazinamide.
- analyse : ADN, Oligodésoxyribonucléotides.
- génétique : ADN, Oligodésoxyribonucléotides.
- instrumentation : Acides picoliniques, Données de séquences moléculaires, Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, DNA (analysis), DNA (genetics), Molecular Sequence Data, Oligodeoxyribonucleotides (analysis), Oligodeoxyribonucleotides (genetics), Picolinic Acids, Pyrazinamide (analogs & derivatives), Reproducibility of Results, Solutions, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation).
- MESH :
- chemical , analogs & derivatives : Pyrazinamide.
- chemical , analysis : DNA, Oligodeoxyribonucleotides.
- chemical , genetics : DNA, Oligodeoxyribonucleotides.
- instrumentation : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Base Sequence, Molecular Sequence Data, Picolinic Acids, Reproducibility of Results, Solutions.
Abstract
A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.
DOI: 10.1002/rcm.1404
PubMed: 15052561
Affiliations:
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pubmed:15052561Le document en format XML
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China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name></author><author><name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name></author><author><name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:15052561</idno><idno type="pmid">15052561</idno><idno type="doi">10.1002/rcm.1404</idno><idno type="wicri:Area/PubMed/Corpus">002396</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002396</idno><idno type="wicri:Area/PubMed/Curation">002396</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002396</idno><idno type="wicri:Area/PubMed/Checkpoint">002287</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002287</idno><idno type="wicri:Area/Ncbi/Merge">000273</idno><idno type="wicri:Area/Ncbi/Curation">000273</idno><idno type="wicri:Area/Ncbi/Checkpoint">000273</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.</title><author><name sortKey="Zhou, Lihua" sort="Zhou, Lihua" uniqKey="Zhou L" first="Lihua" last="Zhou">Lihua Zhou</name><affiliation wicri:level="1"><nlm:affiliation>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong, P.R. China.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong</wicri:regionArea><wicri:noRegion>Guangdong</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name></author><author><name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name></author><author><name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name></author></analytic><series><title level="j">Rapid communications in mass spectrometry : RCM</title><idno type="ISSN">0951-4198</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA (analysis)</term><term>DNA (genetics)</term><term>Molecular Sequence Data</term><term>Oligodeoxyribonucleotides (analysis)</term><term>Oligodeoxyribonucleotides (genetics)</term><term>Picolinic Acids</term><term>Pyrazinamide (analogs & derivatives)</term><term>Reproducibility of Results</term><term>Solutions</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>ADN (génétique)</term><term>Acides picoliniques</term><term>Données de séquences moléculaires</term><term>Oligodésoxyribonucléotides (analyse)</term><term>Oligodésoxyribonucléotides (génétique)</term><term>Pyrazinamide (analogues et dérivés)</term><term>Reproductibilité des résultats</term><term>Solutions</term><term>Spectrométrie de masse MALDI (instrumentation)</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Pyrazinamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Pyrazinamide</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Molecular Sequence Data</term><term>Picolinic Acids</term><term>Reproducibility of Results</term><term>Solutions</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Acides picoliniques</term><term>Données de séquences moléculaires</term><term>Reproductibilité des résultats</term><term>Solutions</term><term>Spectrométrie de masse MALDI</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name><name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name><name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Lihua" sort="Zhou, Lihua" uniqKey="Zhou L" first="Lihua" last="Zhou">Lihua Zhou</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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