Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.
Identifieur interne : 000236 ( Ncbi/Checkpoint ); précédent : 000235; suivant : 000237Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.
Auteurs : P F Lehmann ; D. Lin ; B A LaskerSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 1992.
Descripteurs français
- KwdFr :
- MESH :
- génétique : ADN fongique, Candida.
- isolement et purification : Candida.
- Amplification de gène, Candida, Données de séquences moléculaires, Génotype, Humains, Polymorphisme génétique, Réaction de polymérisation en chaîne, Spécificité d'espèce, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : DNA, Fungal.
- classification : Candida.
- genetics : Candida.
- isolation & purification : Candida.
- Base Sequence, Gene Amplification, Genotype, Humans, Molecular Sequence Data, Polymerase Chain Reaction, Polymorphism, Genetic, Species Specificity.
Abstract
Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction products were observed for isolates of C. albicans, C. lusitaniae, C. tropicalis, and Torulopsis (Candida) glabrata. The RAPD analysis differentiated a physiologically homogeneous panel of C. parapsilosis into three distinct groups and showed genetic diversity within C. haemulonii. Intraspecies DNA-length polymorphisms were seen for RAPD profiles derived from different isolates of each species. Analysis of RAPDs from a panel of C. albicans, which included 16 laboratory derivatives of two reference strains, showed that the profiles of unrelated strains differed and that the derivatives of each reference strain were identifiable. Minor differences in the RAPD profiles, suggestive of mutations that had occurred during the long-term maintenance of the strains, were detected. Because of its ease and reliability, RAPD analysis should be useful in providing genotypic characters for taxonomic descriptions, for confirming the identities of stock isolates, for typing Candida species in epidemiologic investigations, and for use in the rapid identification of pathogenic fungi.
PubMed: 1452710
Affiliations:
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Lin</name></author><author><name sortKey="Lasker, B A" sort="Lasker, B A" uniqKey="Lasker B" first="B A" last="Lasker">B A Lasker</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1992">1992</date><idno type="RBID">pubmed:1452710</idno><idno type="pmid">1452710</idno><idno type="wicri:Area/PubMed/Corpus">002937</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002937</idno><idno type="wicri:Area/PubMed/Curation">002937</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002937</idno><idno type="wicri:Area/PubMed/Checkpoint">002810</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002810</idno><idno type="wicri:Area/Ncbi/Merge">000236</idno><idno type="wicri:Area/Ncbi/Curation">000236</idno><idno type="wicri:Area/Ncbi/Checkpoint">000236</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA.</title><author><name sortKey="Lehmann, P F" sort="Lehmann, P F" uniqKey="Lehmann P" first="P F" last="Lehmann">P F Lehmann</name><affiliation><nlm:affiliation>Department of Microbiology, Medical College of Ohio, Toledo 43699-0008.</nlm:affiliation><wicri:noCountry code="subField">Toledo 43699-0008</wicri:noCountry></affiliation></author><author><name sortKey="Lin, D" sort="Lin, D" uniqKey="Lin D" first="D" last="Lin">D. Lin</name></author><author><name sortKey="Lasker, B A" sort="Lasker, B A" uniqKey="Lasker B" first="B A" last="Lasker">B A Lasker</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Candida (classification)</term><term>Candida (genetics)</term><term>Candida (isolation & purification)</term><term>DNA, Fungal (genetics)</term><term>Gene Amplification</term><term>Genotype</term><term>Humans</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Genetic</term><term>Species Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>Amplification de gène</term><term>Candida ()</term><term>Candida (génétique)</term><term>Candida (isolement et purification)</term><term>Données de séquences moléculaires</term><term>Génotype</term><term>Humains</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité d'espèce</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Candida</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Candida</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>Candida</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Candida</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Candida</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Gene Amplification</term><term>Genotype</term><term>Humans</term><term>Molecular Sequence Data</term><term>Polymerase Chain Reaction</term><term>Polymorphism, Genetic</term><term>Species Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amplification de gène</term><term>Candida</term><term>Données de séquences moléculaires</term><term>Génotype</term><term>Humains</term><term>Polymorphisme génétique</term><term>Réaction de polymérisation en chaîne</term><term>Spécificité d'espèce</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Random amplified polymorphic DNA (RAPD) was used to better characterize the genotypic relatedness among medically important Candida species. By using short oligomer primers (10-mers) with arbitrarily chosen sequences in the polymerase chain reaction, distinctive and reproducible sets of polymerase chain reaction products were observed for isolates of C. albicans, C. lusitaniae, C. tropicalis, and Torulopsis (Candida) glabrata. The RAPD analysis differentiated a physiologically homogeneous panel of C. parapsilosis into three distinct groups and showed genetic diversity within C. haemulonii. Intraspecies DNA-length polymorphisms were seen for RAPD profiles derived from different isolates of each species. Analysis of RAPDs from a panel of C. albicans, which included 16 laboratory derivatives of two reference strains, showed that the profiles of unrelated strains differed and that the derivatives of each reference strain were identifiable. Minor differences in the RAPD profiles, suggestive of mutations that had occurred during the long-term maintenance of the strains, were detected. Because of its ease and reliability, RAPD analysis should be useful in providing genotypic characters for taxonomic descriptions, for confirming the identities of stock isolates, for typing Candida species in epidemiologic investigations, and for use in the rapid identification of pathogenic fungi.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Lasker, B A" sort="Lasker, B A" uniqKey="Lasker B" first="B A" last="Lasker">B A Lasker</name><name sortKey="Lehmann, P F" sort="Lehmann, P F" uniqKey="Lehmann P" first="P F" last="Lehmann">P F Lehmann</name><name sortKey="Lin, D" sort="Lin, D" uniqKey="Lin D" first="D" last="Lin">D. Lin</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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