Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea).
Identifieur interne : 000056 ( Ncbi/Checkpoint ); précédent : 000055; suivant : 000057Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea).
Auteurs : R. Terauchi [Allemagne] ; G. KahlSource :
- Molecular & general genetics : MGG [ 0026-8925 ] ; 2000.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (), Données de séquences moléculaires, Exons, Glucose 6-phosphate isomerase (génétique), Introns, Liliaceae (génétique), Modèles génétiques, Phenylalanine ammonia-lyase (génétique), Réaction de polymérisation en chaîne (), Régions promotrices (génétique), Similitude de séquences d'acides nucléiques, Séquence nucléotidique.
- MESH :
- génétique : Glucose 6-phosphate isomerase, Liliaceae, Phenylalanine ammonia-lyase.
- Analyse de séquence d'ADN, Données de séquences moléculaires, Exons, Introns, Modèles génétiques, Réaction de polymérisation en chaîne, Régions promotrices (génétique), Similitude de séquences d'acides nucléiques, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Exons, Glucose-6-Phosphate Isomerase (genetics), Introns, Liliaceae (genetics), Models, Genetic, Molecular Sequence Data, Phenylalanine Ammonia-Lyase (genetics), Polymerase Chain Reaction (methods), Promoter Regions, Genetic, Sequence Analysis, DNA (methods), Sequence Homology, Nucleic Acid.
- MESH :
- chemical , genetics : Glucose-6-Phosphate Isomerase, Phenylalanine Ammonia-Lyase.
- genetics : Liliaceae.
- methods : Polymerase Chain Reaction, Sequence Analysis, DNA.
- Base Sequence, Exons, Introns, Models, Genetic, Molecular Sequence Data, Promoter Regions, Genetic, Sequence Homology, Nucleic Acid.
Abstract
Using a modified TAIL-PCR technique, the 5'-flanking regions of the phenylalanine ammonia lyase (Pal) genes of a yam species, Dioscorea bulbifera, and the phosphoglucose isomerase (Pgi) gene of D. tokoro were successfully isolated. Two novel modifications of the TAIL-PCR procedure introduced here, namely (1) the use of a battery of random 10-mers (RAPD primers) as short arbitrary primers, and (2) the use of a total of five nested, gene-specific primers, allow the rapid isolation of the 5'-flanking region of any gene from organisms with large genomes. Isolated 5'-flanking regions were fused to the gus gene, and tested for transient expression in tobacco BY2 cells. All the isolated 5'-flanking regions were shown to drive reporter gene expression. Three Pal promoters responded to salicylic acid, presumably as a result of the binding of a MYB transcriptional activator to the multiple MREs (Myb Recognition Elements) present in these regions.
DOI: 10.1007/s004380051201
PubMed: 10821191
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002589
- to stream PubMed, to step Curation: 002589
- to stream PubMed, to step Checkpoint: 002438
- to stream Ncbi, to step Merge: 000056
- to stream Ncbi, to step Curation: 000056
Links to Exploration step
pubmed:10821191Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea).</title><author><name sortKey="Terauchi, R" sort="Terauchi, R" uniqKey="Terauchi R" first="R" last="Terauchi">R. Terauchi</name><affiliation wicri:level="3"><nlm:affiliation>Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt am Main, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Kahl, G" sort="Kahl, G" uniqKey="Kahl G" first="G" last="Kahl">G. Kahl</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2000">2000</date><idno type="RBID">pubmed:10821191</idno><idno type="pmid">10821191</idno><idno type="doi">10.1007/s004380051201</idno><idno type="wicri:Area/PubMed/Corpus">002589</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002589</idno><idno type="wicri:Area/PubMed/Curation">002589</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002589</idno><idno type="wicri:Area/PubMed/Checkpoint">002438</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002438</idno><idno type="wicri:Area/Ncbi/Merge">000056</idno><idno type="wicri:Area/Ncbi/Curation">000056</idno><idno type="wicri:Area/Ncbi/Checkpoint">000056</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea).</title><author><name sortKey="Terauchi, R" sort="Terauchi, R" uniqKey="Terauchi R" first="R" last="Terauchi">R. Terauchi</name><affiliation wicri:level="3"><nlm:affiliation>Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt am Main, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Plant Molecular Biology, Biocentre, University of Frankfurt, Frankfurt am Main</wicri:regionArea><placeName><region type="land" nuts="1">Hesse (Land)</region><region type="district" nuts="2">District de Darmstadt</region><settlement type="city">Francfort-sur-le-Main</settlement></placeName></affiliation></author><author><name sortKey="Kahl, G" sort="Kahl, G" uniqKey="Kahl G" first="G" last="Kahl">G. Kahl</name></author></analytic><series><title level="j">Molecular & general genetics : MGG</title><idno type="ISSN">0026-8925</idno><imprint><date when="2000" type="published">2000</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Exons</term><term>Glucose-6-Phosphate Isomerase (genetics)</term><term>Introns</term><term>Liliaceae (genetics)</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Phenylalanine Ammonia-Lyase (genetics)</term><term>Polymerase Chain Reaction (methods)</term><term>Promoter Regions, Genetic</term><term>Sequence Analysis, DNA (methods)</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN ()</term><term>Données de séquences moléculaires</term><term>Exons</term><term>Glucose 6-phosphate isomerase (génétique)</term><term>Introns</term><term>Liliaceae (génétique)</term><term>Modèles génétiques</term><term>Phenylalanine ammonia-lyase (génétique)</term><term>Réaction de polymérisation en chaîne ()</term><term>Régions promotrices (génétique)</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence nucléotidique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glucose-6-Phosphate Isomerase</term><term>Phenylalanine Ammonia-Lyase</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Liliaceae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glucose 6-phosphate isomerase</term><term>Liliaceae</term><term>Phenylalanine ammonia-lyase</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Exons</term><term>Introns</term><term>Models, Genetic</term><term>Molecular Sequence Data</term><term>Promoter Regions, Genetic</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Données de séquences moléculaires</term><term>Exons</term><term>Introns</term><term>Modèles génétiques</term><term>Réaction de polymérisation en chaîne</term><term>Régions promotrices (génétique)</term><term>Similitude de séquences d'acides nucléiques</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Using a modified TAIL-PCR technique, the 5'-flanking regions of the phenylalanine ammonia lyase (Pal) genes of a yam species, Dioscorea bulbifera, and the phosphoglucose isomerase (Pgi) gene of D. tokoro were successfully isolated. Two novel modifications of the TAIL-PCR procedure introduced here, namely (1) the use of a battery of random 10-mers (RAPD primers) as short arbitrary primers, and (2) the use of a total of five nested, gene-specific primers, allow the rapid isolation of the 5'-flanking region of any gene from organisms with large genomes. Isolated 5'-flanking regions were fused to the gus gene, and tested for transient expression in tobacco BY2 cells. All the isolated 5'-flanking regions were shown to drive reporter gene expression. Three Pal promoters responded to salicylic acid, presumably as a result of the binding of a MYB transcriptional activator to the multiple MREs (Myb Recognition Elements) present in these regions.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>District de Darmstadt</li><li>Hesse (Land)</li></region><settlement><li>Francfort-sur-le-Main</li></settlement></list><tree><noCountry><name sortKey="Kahl, G" sort="Kahl, G" uniqKey="Kahl G" first="G" last="Kahl">G. Kahl</name></noCountry><country name="Allemagne"><region name="Hesse (Land)"><name sortKey="Terauchi, R" sort="Terauchi, R" uniqKey="Terauchi R" first="R" last="Terauchi">R. Terauchi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000056 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 000056 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:10821191
|texte= Rapid isolation of promoter sequences by TAIL-PCR: the 5'-flanking regions of Pal and Pgi genes from yams (Dioscorea).
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:10821191" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |