Genomic Organization, Nucleotide Sequence, Biophysical Properties, and Localization of the Voltage-Gated K+ Channel Gene KCNA4/Kv1.4 to Mouse Chromosome 2/Human 11p14 and Mapping of KCNC1/Kv3.1 to Mouse 7/Human 11p14.3-p15.2 and KCNA1/Kv1.1 to Human 12p13
Identifieur interne : 004505 ( Main/Merge ); précédent : 004504; suivant : 004506Genomic Organization, Nucleotide Sequence, Biophysical Properties, and Localization of the Voltage-Gated K+ Channel Gene KCNA4/Kv1.4 to Mouse Chromosome 2/Human 11p14 and Mapping of KCNC1/Kv3.1 to Mouse 7/Human 11p14.3-p15.2 and KCNA1/Kv1.1 to Human 12p13
Auteurs : Randy S. Wymore [États-Unis] ; Julie R. Korenberg [États-Unis] ; Keith D. Kinoshita [États-Unis] ; Jayashree Aiyar [États-Unis] ; Christopher Coyne [États-Unis] ; Xiao-Ning Chen [États-Unis] ; Carolyn M. Hustad [États-Unis] ; Neal G. Copeland [États-Unis] ; George A. Gutman [États-Unis] ; Nancy A. Jenkins [États-Unis] ; K. George Chandy [États-Unis]Source :
- Genomics [ 0888-7543 ] ; 1994.
Abstract
Abstract: A genomic clone encoding the Shaker-related potassium channel gene, Kcna4/mKv1.4 , was isolated from mice. Its coding region is contained in a single exon, encodes a protein of 654 amino acids, and shares ∼91% nucleotide sequence identity with human KCNA4/hKv.14. We show that 0.8 kb of the 5′ noncoding region (NCR), the entire protein coding region (∼2.0 kb), and all of the known 3′ NCR (∼ 1.1 kb) are contained within a single exon; the remaining 0.5 kb of the 5′ NCR is separated from this exon by a 3.4-kb intron. The sequenced genomic region thus accounts for essentially all of the longest known transcript (4.5 kb), although the precise ends of this transcript have not been defined. The 3′ NCR contains several ATTTA and ATTTG motifs that are thought to destabilize mRNAs, and these are also present in rat, bovine, and human Kcna4/Kv1.4 cDNAs. It also contains three conserved polyadenylation signals, alternate utilization of which could generate mRNAs of differing stabilities. The 5′ NCR of Kcna4/mKv1.4 may also serve to regulate channel expression. This region is ∼85% identical to KCNA4/hKv1.4 and contains eight consensus translation start sites [(G, A)NNATG] that, based on the 5′-3′ scanning model, would lead to a lowering of translational efficiency. The shortest Kcna4/Kv1.4 transcript (2.4 kb) can contain at most 400 bp of NCR and should lack the 3′ ATTTAs and most of the 5′ ATGs; this transcript might therefore exhibit increased stability and translational efficiency. The Kcna4/mKv1.4 channel exhibited biophysical and pharmacological properties indistinguishable from its rat and human homologues. Kcna4/mKv1.4 lies on mouse chromosome 2, near the Fshb locus, and in humans on the proximal half of chromosome 11p14 near human FSHB. Another K+ channel gene, Kcnc1/mKv3.1, lies ∼1.8 cM from the Myod-1 gene on mouse chromosome 7, and in situ hybridization localizes KCNC1/hKv3.1 to the homologous region on human chromosome 11p14.3-p15.2. A third gene, KCNA1/hKv1.1, was mapped to human 12p13.
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DOI: 10.1006/geno.1994.1153
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<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genomic Organization, Nucleotide Sequence, Biophysical Properties, and Localization of the Voltage-Gated K+ Channel Gene KCNA4/Kv1.4 to Mouse Chromosome 2/Human 11p14 and Mapping of KCNC1/Kv3.1 to Mouse 7/Human 11p14.3-p15.2 and KCNA1/Kv1.1 to Human 12p13</title><author><name sortKey="Wymore, Randy S" sort="Wymore, Randy S" uniqKey="Wymore R" first="Randy S." last="Wymore">Randy S. Wymore</name></author><author><name sortKey="Korenberg, Julie R" sort="Korenberg, Julie R" uniqKey="Korenberg J" first="Julie R." last="Korenberg">Julie R. Korenberg</name></author><author><name sortKey="Kinoshita, Keith D" sort="Kinoshita, Keith D" uniqKey="Kinoshita K" first="Keith D." last="Kinoshita">Keith D. Kinoshita</name></author><author><name sortKey="Aiyar, Jayashree" sort="Aiyar, Jayashree" uniqKey="Aiyar J" first="Jayashree" last="Aiyar">Jayashree Aiyar</name></author><author><name sortKey="Coyne, Christopher" sort="Coyne, Christopher" uniqKey="Coyne C" first="Christopher" last="Coyne">Christopher Coyne</name></author><author><name sortKey="Chen, Xiao Ning" sort="Chen, Xiao Ning" uniqKey="Chen X" first="Xiao-Ning" last="Chen">Xiao-Ning Chen</name></author><author><name sortKey="Hustad, Carolyn M" sort="Hustad, Carolyn M" uniqKey="Hustad C" first="Carolyn M." last="Hustad">Carolyn M. Hustad</name></author><author><name sortKey="Copeland, Neal G" sort="Copeland, Neal G" uniqKey="Copeland N" first="Neal G." last="Copeland">Neal G. Copeland</name></author><author><name sortKey="Gutman, George A" sort="Gutman, George A" uniqKey="Gutman G" first="George A." last="Gutman">George A. Gutman</name></author><author><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A." last="Jenkins">Nancy A. Jenkins</name></author><author><name sortKey="Chandy, K George" sort="Chandy, K George" uniqKey="Chandy K" first="K. George" last="Chandy">K. George Chandy</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A6DEE80769670AF5FE9C2DB106B2E9EFF5B62C5B</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1006/geno.1994.1153</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-T7Q1J9W0-Z/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001E75</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001E75</idno><idno type="wicri:Area/Istex/Curation">001E75</idno><idno type="wicri:Area/Istex/Checkpoint">001A79</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001A79</idno><idno type="wicri:doubleKey">0888-7543:1994:Wymore R:genomic:organization:nucleotide</idno><idno type="wicri:Area/Main/Merge">004505</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Genomic Organization, Nucleotide Sequence, Biophysical Properties, and Localization of the Voltage-Gated K+ Channel Gene KCNA4/Kv1.4 to Mouse Chromosome 2/Human 11p14 and Mapping of KCNC1/Kv3.1 to Mouse 7/Human 11p14.3-p15.2 and KCNA1/Kv1.1 to Human 12p13</title><author><name sortKey="Wymore, Randy S" sort="Wymore, Randy S" uniqKey="Wymore R" first="Randy S." last="Wymore">Randy S. Wymore</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Korenberg, Julie R" sort="Korenberg, Julie R" uniqKey="Korenberg J" first="Julie R." last="Korenberg">Julie R. Korenberg</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Kinoshita, Keith D" sort="Kinoshita, Keith D" uniqKey="Kinoshita K" first="Keith D." last="Kinoshita">Keith D. Kinoshita</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Aiyar, Jayashree" sort="Aiyar, Jayashree" uniqKey="Aiyar J" first="Jayashree" last="Aiyar">Jayashree Aiyar</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Coyne, Christopher" sort="Coyne, Christopher" uniqKey="Coyne C" first="Christopher" last="Coyne">Christopher Coyne</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Chen, Xiao Ning" sort="Chen, Xiao Ning" uniqKey="Chen X" first="Xiao-Ning" last="Chen">Xiao-Ning Chen</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Hustad, Carolyn M" sort="Hustad, Carolyn M" uniqKey="Hustad C" first="Carolyn M." last="Hustad">Carolyn M. Hustad</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Copeland, Neal G" sort="Copeland, Neal G" uniqKey="Copeland N" first="Neal G." last="Copeland">Neal G. Copeland</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Gutman, George A" sort="Gutman, George A" uniqKey="Gutman G" first="George A." last="Gutman">George A. Gutman</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A." last="Jenkins">Nancy A. Jenkins</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Chandy, K George" sort="Chandy, K George" uniqKey="Chandy K" first="K. George" last="Chandy">K. George Chandy</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Departments of Physiology and Biophysics and Microbiology and Molecular Genetics, University of California, Irvine, California 92717; Division of Medical Genetics, Cedars-Sinai Medical Center, Los Angeles, California 90048; and Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Genomics</title><title level="j" type="abbrev">YGENO</title><idno type="ISSN">0888-7543</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1994">1994</date><biblScope unit="volume">20</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="191">191</biblScope><biblScope unit="page" to="202">202</biblScope></imprint><idno type="ISSN">0888-7543</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0888-7543</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A genomic clone encoding the Shaker-related potassium channel gene, Kcna4/mKv1.4 , was isolated from mice. Its coding region is contained in a single exon, encodes a protein of 654 amino acids, and shares ∼91% nucleotide sequence identity with human KCNA4/hKv.14. We show that 0.8 kb of the 5′ noncoding region (NCR), the entire protein coding region (∼2.0 kb), and all of the known 3′ NCR (∼ 1.1 kb) are contained within a single exon; the remaining 0.5 kb of the 5′ NCR is separated from this exon by a 3.4-kb intron. The sequenced genomic region thus accounts for essentially all of the longest known transcript (4.5 kb), although the precise ends of this transcript have not been defined. The 3′ NCR contains several ATTTA and ATTTG motifs that are thought to destabilize mRNAs, and these are also present in rat, bovine, and human Kcna4/Kv1.4 cDNAs. It also contains three conserved polyadenylation signals, alternate utilization of which could generate mRNAs of differing stabilities. The 5′ NCR of Kcna4/mKv1.4 may also serve to regulate channel expression. This region is ∼85% identical to KCNA4/hKv1.4 and contains eight consensus translation start sites [(G, A)NNATG] that, based on the 5′-3′ scanning model, would lead to a lowering of translational efficiency. The shortest Kcna4/Kv1.4 transcript (2.4 kb) can contain at most 400 bp of NCR and should lack the 3′ ATTTAs and most of the 5′ ATGs; this transcript might therefore exhibit increased stability and translational efficiency. The Kcna4/mKv1.4 channel exhibited biophysical and pharmacological properties indistinguishable from its rat and human homologues. Kcna4/mKv1.4 lies on mouse chromosome 2, near the Fshb locus, and in humans on the proximal half of chromosome 11p14 near human FSHB. Another K+ channel gene, Kcnc1/mKv3.1, lies ∼1.8 cM from the Myod-1 gene on mouse chromosome 7, and in situ hybridization localizes KCNC1/hKv3.1 to the homologous region on human chromosome 11p14.3-p15.2. A third gene, KCNA1/hKv1.1, was mapped to human 12p13.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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