Secretion and Antigenicity of Hepatitis B Virus Small Envelope Proteins Lacking Cysteines in the Major Antigenic Region
Identifieur interne : 004200 ( Main/Merge ); précédent : 004199; suivant : 004201Secretion and Antigenicity of Hepatitis B Virus Small Envelope Proteins Lacking Cysteines in the Major Antigenic Region
Auteurs : Constance M. T. Mangold [Allemagne] ; Felix Unckell [Allemagne] ; Margaret Werr [Allemagne] ; Rolf E. Streeck [Allemagne]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: Disulfide bonds are of crucial importance for the structure and antigenic properties of the hepatitis B virus (HBV) envelope. We have evaluated the role of the eight highly conserved cysteines of the major antigenic region for assembly, secretion, and antigenicity of the envelope proteins. Mutants carrying single or multiple substitutions of alanine for cysteine were analyzed using epitope tagging and transient expression in COS-7 cells. The only single cysteines found to be indispensable for efficient secretion were Cys-107 and Cys-138, but double mutation of Cys-137 and Cys-139 also created a block to secretion. Poorly secreted mutants formed aberrant oligomeric structures. The antigenicity of the secreted or intracellularly retained mutants was analyzed using a panel of six monoclonal antibodies recognizing group- and subtype-specific determinants. We demonstrate that Cys-107 is critical for the structure of the group determinant a1 whereas Cys-147, previously implicated in intramolecular disulfide bonding, is dispensable. Mutant proteins lacking Cys-121 and -124, -137, -147, or 149 have grossly distorted structures of the y subtype determinant. Our data raise the possibility that HBV strains carrying cysteine mutations are nonreactive in hepatitis B surface antigen-specific immunoassays.
Url:
DOI: 10.1006/viro.1995.1435
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001690
- to stream Istex, to step Curation: 001690
- to stream Istex, to step Checkpoint: 001830
Links to Exploration step
ISTEX:B902B9CFC6C0D7B2AE4EF8E1359F577D45DB6026Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Secretion and Antigenicity of Hepatitis B Virus Small Envelope Proteins Lacking Cysteines in the Major Antigenic Region</title>
<author><name sortKey="Mangold, Constance M T" sort="Mangold, Constance M T" uniqKey="Mangold C" first="Constance M. T." last="Mangold">Constance M. T. Mangold</name>
</author>
<author><name sortKey="Unckell, Felix" sort="Unckell, Felix" uniqKey="Unckell F" first="Felix" last="Unckell">Felix Unckell</name>
</author>
<author><name sortKey="Werr, Margaret" sort="Werr, Margaret" uniqKey="Werr M" first="Margaret" last="Werr">Margaret Werr</name>
</author>
<author><name sortKey="Streeck, Rolf E" sort="Streeck, Rolf E" uniqKey="Streeck R" first="Rolf E." last="Streeck">Rolf E. Streeck</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:B902B9CFC6C0D7B2AE4EF8E1359F577D45DB6026</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1006/viro.1995.1435</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-06C3S3JK-4/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001690</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001690</idno>
<idno type="wicri:Area/Istex/Curation">001690</idno>
<idno type="wicri:Area/Istex/Checkpoint">001830</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001830</idno>
<idno type="wicri:doubleKey">0042-6822:1995:Mangold C:secretion:and:antigenicity</idno>
<idno type="wicri:Area/Main/Merge">004200</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Secretion and Antigenicity of Hepatitis B Virus Small Envelope Proteins Lacking Cysteines in the Major Antigenic Region</title>
<author><name sortKey="Mangold, Constance M T" sort="Mangold, Constance M T" uniqKey="Mangold C" first="Constance M. T." last="Mangold">Constance M. T. Mangold</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institut für Medizinische Mikrobiologie, Johannes Gutenberg-Universität Mainz, Augustusplatz, D-55101 Mainz</wicri:regionArea>
<wicri:noRegion>55101 Mainz</wicri:noRegion>
<wicri:noRegion>D-55101 Mainz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Unckell, Felix" sort="Unckell, Felix" uniqKey="Unckell F" first="Felix" last="Unckell">Felix Unckell</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institut für Medizinische Mikrobiologie, Johannes Gutenberg-Universität Mainz, Augustusplatz, D-55101 Mainz</wicri:regionArea>
<wicri:noRegion>55101 Mainz</wicri:noRegion>
<wicri:noRegion>D-55101 Mainz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Werr, Margaret" sort="Werr, Margaret" uniqKey="Werr M" first="Margaret" last="Werr">Margaret Werr</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institut für Medizinische Mikrobiologie, Johannes Gutenberg-Universität Mainz, Augustusplatz, D-55101 Mainz</wicri:regionArea>
<wicri:noRegion>55101 Mainz</wicri:noRegion>
<wicri:noRegion>D-55101 Mainz</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Streeck, Rolf E" sort="Streeck, Rolf E" uniqKey="Streeck R" first="Rolf E." last="Streeck">Rolf E. Streeck</name>
<affiliation wicri:level="1"><country xml:lang="fr">Allemagne</country>
<wicri:regionArea>Institut für Medizinische Mikrobiologie, Johannes Gutenberg-Universität Mainz, Augustusplatz, D-55101 Mainz</wicri:regionArea>
<wicri:noRegion>55101 Mainz</wicri:noRegion>
<wicri:noRegion>D-55101 Mainz</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1995">1995</date>
<biblScope unit="volume">211</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="535">535</biblScope>
<biblScope unit="page" to="543">543</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Disulfide bonds are of crucial importance for the structure and antigenic properties of the hepatitis B virus (HBV) envelope. We have evaluated the role of the eight highly conserved cysteines of the major antigenic region for assembly, secretion, and antigenicity of the envelope proteins. Mutants carrying single or multiple substitutions of alanine for cysteine were analyzed using epitope tagging and transient expression in COS-7 cells. The only single cysteines found to be indispensable for efficient secretion were Cys-107 and Cys-138, but double mutation of Cys-137 and Cys-139 also created a block to secretion. Poorly secreted mutants formed aberrant oligomeric structures. The antigenicity of the secreted or intracellularly retained mutants was analyzed using a panel of six monoclonal antibodies recognizing group- and subtype-specific determinants. We demonstrate that Cys-107 is critical for the structure of the group determinant a1 whereas Cys-147, previously implicated in intramolecular disulfide bonding, is dispensable. Mutant proteins lacking Cys-121 and -124, -137, -147, or 149 have grossly distorted structures of the y subtype determinant. Our data raise the possibility that HBV strains carrying cysteine mutations are nonreactive in hepatitis B surface antigen-specific immunoassays.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004200 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 004200 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Merge |type= RBID |clé= ISTEX:B902B9CFC6C0D7B2AE4EF8E1359F577D45DB6026 |texte= Secretion and Antigenicity of Hepatitis B Virus Small Envelope Proteins Lacking Cysteines in the Major Antigenic Region }}
This area was generated with Dilib version V0.6.33. |