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Differential changes in rat brain nitric oxide synthase in vivo and in vitro by methylmercury

Identifieur interne : 003C27 ( Main/Merge ); précédent : 003C26; suivant : 003C28

Differential changes in rat brain nitric oxide synthase in vivo and in vitro by methylmercury

Auteurs : Masaru Shinyashiki [Japon] ; Yoshito Kumagai [Japon] ; Hiromi Nakajima [Japon] ; Jun Nagafune [Japon] ; Shino Homma-Takeda [Japon] ; Masaru Sagai [Japon] ; Nobuhiro Shimojo [Japon]

Source :

Brain Research [ 0006-8993 ] ; 1998.

RBID : ISTEX:DF9D5B1F6BF3A2ECD438635FB4C0C901141D6D12

English descriptors

KwdEn :
- Enzyme activity, Inhibition, Mercuric chloride, Methylmercuric chloride, Nitric oxide synthase, Thiol compounds.
Teeft :
- Brain research, Cerebellar, Cerebellum, Cerebrum, Continuous exposure, Covalent modification, Dismutase, Enzyme, Enzyme activity, Enzyme loss, Enzyme preparation, First injection, Gene expression, Immunoblot analysis, Incubation mixture, Inos, Intracellular, Isozymes, Kgy1 dayy1, Macrophage, Macrophage inos, Manganese superoxide dismutase, Mercurials, Mercuric, Mercuric chloride, Mercuric compounds, Mercury compounds, Methylmercuric chloride, Methylmercury, Mgy1, Miny1, Mrna, Neuron, Nitric, Nitric oxide, Nitric oxide synthase, Nmol, Nmol miny1 mgy1, Nnos, Nnos activity, Nnos protein, Oxide, Pharmacol, Primer, Protein levels, Purkinje cells, Reaction mixture, Shinyashiki, Slight modification, Sulfhydryl, Sulfhydryl compounds, Supernatant, Superoxide, Synthase, Thiol, Toxicol, Tsukuba, Western blot analysis.

Abstract

Abstract: Alterations in mRNA level, protein content and enzyme activity for nitric oxide synthase (NOS) in the cerebrum and cerebellum during a continuous exposure of neurotoxic metal, methylmercury, were examined in Wistar rats. Subcutaneous (s.c.) administration of methylmercuric chloride (MMC, 10 mg kg−1 day−1, 8 days) resulted in significant increases with time of NOS activities in the cerebrum (1.6–1.9-fold, 5–8 days) and cerebellum (1.4-fold, 8 days). RT-PCR and immunoblot analyses indicated that the increase in the enzyme activity caused by this metal appears to be due to increase in protein levels of neuronal NOS (nNOS), but not inducible NOS (iNOS) because little appreciable mRNA and protein for iNOS were seen during MMC exposure. The direct effect of mercuric compounds on nNOS activity in vitro was evaluated using 20,000×g supernatant from rat cerebellum homogenate. In contrast to the in vivo observation, inorganic-, alkyl-, and aryl-mercuric compound showed potent inhibition of nNOS activity with IC50 values of 11–43 μM, whereas dimethylmercury (DMM) was without effect on the enzyme activity. Further experiments indicated that the inhibition of nNOS by organomercurial occurred via thiol modification.

Url:

https://api.istex.fr/ark:/67375/6H6-N6Z0091G-T/fulltext.pdf

DOI: 10.1016/S0006-8993(98)00400-4

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Alterations in mRNA level, protein content and enzyme activity for nitric oxide synthase (NOS) in the cerebrum and cerebellum during a continuous exposure of neurotoxic metal, methylmercury, were examined in Wistar rats. Subcutaneous (s.c.) administration of methylmercuric chloride (MMC, 10 mg kg−1 day−1, 8 days) resulted in significant increases with time of NOS activities in the cerebrum (1.6–1.9-fold, 5–8 days) and cerebellum (1.4-fold, 8 days). RT-PCR and immunoblot analyses indicated that the increase in the enzyme activity caused by this metal appears to be due to increase in protein levels of neuronal NOS (nNOS), but not inducible NOS (iNOS) because little appreciable mRNA and protein for iNOS were seen during MMC exposure. The direct effect of mercuric compounds on nNOS activity in vitro was evaluated using 20,000×g supernatant from rat cerebellum homogenate. In contrast to the in vivo observation, inorganic-, alkyl-, and aryl-mercuric compound showed potent inhibition of nNOS activity with IC50 values of 11–43 μM, whereas dimethylmercury (DMM) was without effect on the enzyme activity. Further experiments indicated that the inhibition of nNOS by organomercurial occurred via thiol modification.</div>
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Differential changes in rat brain nitric oxide synthase in vivo and in vitro by methylmercury

Differential changes in rat brain nitric oxide synthase in vivo and in vitro by methylmercury

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

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