MersV1, Main, Merge, bibRecord, 003742

Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication

Identifieur interne : 003742 ( Main/Merge ); précédent : 003741; suivant : 003743

Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication

Auteurs : Bing Wang [République populaire de Chine] ; Li-Hua Ke [République populaire de Chine] ; Hong Jiang [République populaire de Chine] ; Chuan-Zhao Li [République populaire de Chine] ; Po Tien [République populaire de Chine]

Source :

Virus Research [ 0168-1702 ] ; 2000.

RBID : ISTEX:2B4B264E60CF598B3DA49F13E7F41F6E5376D766

English descriptors

KwdEn :
- Grass carp hemorrhage virus, Inhibition of replication, Kidney cell line, Peptide library.
Teeft :
- Amino terminus, Beckman model, Biotinylated, Biotinylated gchv, Blotto solution, Cell culture, Cell line, Chinese academy, Clone, Ctenopharyngodon idellus, Electron microscopy, Elsevier science, Escherichia coli, Fuse5, Gchv, Gchv replication, Hemorrhage virus, Hemorrhagic disease, Hunan province, Icosahedral particles, Kidney cell line, Kidney cells, Light microscopy, Microfuge tube, Microtiter plate, Normal atmosphere, Pellet, Peptide, Peptide library, Peptide ligands, Peptide sequence, Phage, Phage display, Phage fuse5, Positive clones, Random library, Random peptide library, Replication, Room temperature, Sodium acetate, Virion, Virus particle, Virus research, Wang.

Abstract

Abstract: Grass carp hemorrhage virus (GCHV), a member of Reoviridae, causes severe hemorrhagic disease of grass carp (Ctenopharyngodon idellus) in China. Icosahedral virions of GCHV were used as to assay the effect of specific peptides for the inhibition of GCHV infectivity. A random nona-peptide library displayed on phage fUSE5 was constructed, and the expressed peptides were fused onto the amino terminus of the minor coat protein III. By biopanning, the fused peptides were bound to the biotinylated GCHV. Phages containing specific peptides bound to GCHV were eluted and amplified in Escherichia coli K91. Three rounds of affinity selection enriched the pool of inhibiting peptides. Sixteen clones which inhibited the replication of GCHV in a grass carp kidney cell line were selected. The TCID50 of GCHV was decreased over 10 000×. Six clones having the strongest inhibitory effect shared the same DNA sequence, with a deduced amino acid sequence of NH2-Leu-Trp-Val-Gly-Gly-Gly-Arg-Asn-Ala-COOH. A synthesized nona-peptide of identical sequence exhibited similar inhibitory activity towards GCHV replication in vitro.

Url:

https://api.istex.fr/ark:/67375/6H6-TQZBX4HD-D/fulltext.pdf

DOI: 10.1016/S0168-1702(00)00132-5

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ISTEX:2B4B264E60CF598B3DA49F13E7F41F6E5376D766

Le document en format XML

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Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication

Selection of a specific peptide from a nona-peptide library for in vitro inhibition of grass carp hemorrhage virus replication

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

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