Selecting optimal antisense reagents
Identifieur interne : 003684 ( Main/Merge ); précédent : 003683; suivant : 003685Selecting optimal antisense reagents
Auteurs : M. Sohail [Royaume-Uni] ; E. M Southern [Royaume-Uni]Source :
- Advanced Drug Delivery Reviews [ 0169-409X ] ; 2000.
English descriptors
- Teeft :
- Accessible, Accessible sites, Antisense, Antisense activity, Antisense oligodeoxynucleotides, Antisense oligonucleotides, Antisense reagents, Assay, Biol, Biotechnol, Cell cultures, Chem, Cleavage, Computer programs, Convincing evidence, Deoxyribozymes, Drug delivery reviews, Effective antisense reagents, Gene expression, Hammerhead ribozyme catalysis, Heteroduplex, Heteroduplex formation, Hybridisation, Large rnas, Mrna, Nucleic, Nucleic acids, Oligonucleotide, Oligonucleotide libraries, Oligonucleotide scanning arrays, Oligonucleotides, Primer extension, Reagent, Ribozyme, Rna, Rnase, Scanning, Scanning array, Scanning arrays, Secondary structure, Sohail, Solid surface, Target mrna, Translation initiation site, Trna.
Abstract
Abstract: Selection of the appropriate target site is crucial to the success of an antisense experiment. The selection is difficult because RNAs fold to form secondary structures, rendering most of the molecule inaccessible to intermolecular base pairing with complementary nucleic acids. Conventional approaches, such as selection by ‘sequence-walking’ or computer-assisted design, have not brought significant success. Several empirical selection methods have been reported, a number of which are summarised in this review. Of notable significance are the ‘global’ methods based on mapping of transcripts with the endoribonuclease H (RNase H) and oligonucleotide scanning arrays.
Url:
DOI: 10.1016/S0169-409X(00)00081-8
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Sohail</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU</wicri:regionArea><orgName type="university">Université d'Oxford</orgName><placeName><settlement type="city">Oxford</settlement><region type="nation">Angleterre</region><region type="région" nuts="1">Oxfordshire</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Royaume-Uni</country></affiliation></author><author><name sortKey="Southern, E M" sort="Southern, E M" uniqKey="Southern E" first="E. M" last="Southern">E. M Southern</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU</wicri:regionArea><orgName type="university">Université d'Oxford</orgName><placeName><settlement type="city">Oxford</settlement><region type="nation">Angleterre</region><region type="région" nuts="1">Oxfordshire</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Advanced Drug Delivery Reviews</title><title level="j" type="abbrev">ADR</title><idno type="ISSN">0169-409X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">44</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="23">23</biblScope><biblScope unit="page" to="34">34</biblScope></imprint><idno type="ISSN">0169-409X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-409X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accessible</term><term>Accessible sites</term><term>Antisense</term><term>Antisense activity</term><term>Antisense oligodeoxynucleotides</term><term>Antisense oligonucleotides</term><term>Antisense reagents</term><term>Assay</term><term>Biol</term><term>Biotechnol</term><term>Cell cultures</term><term>Chem</term><term>Cleavage</term><term>Computer programs</term><term>Convincing evidence</term><term>Deoxyribozymes</term><term>Drug delivery reviews</term><term>Effective antisense reagents</term><term>Gene expression</term><term>Hammerhead ribozyme catalysis</term><term>Heteroduplex</term><term>Heteroduplex formation</term><term>Hybridisation</term><term>Large rnas</term><term>Mrna</term><term>Nucleic</term><term>Nucleic acids</term><term>Oligonucleotide</term><term>Oligonucleotide libraries</term><term>Oligonucleotide scanning arrays</term><term>Oligonucleotides</term><term>Primer extension</term><term>Reagent</term><term>Ribozyme</term><term>Rna</term><term>Rnase</term><term>Scanning</term><term>Scanning array</term><term>Scanning arrays</term><term>Secondary structure</term><term>Sohail</term><term>Solid surface</term><term>Target mrna</term><term>Translation initiation site</term><term>Trna</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Selection of the appropriate target site is crucial to the success of an antisense experiment. The selection is difficult because RNAs fold to form secondary structures, rendering most of the molecule inaccessible to intermolecular base pairing with complementary nucleic acids. Conventional approaches, such as selection by ‘sequence-walking’ or computer-assisted design, have not brought significant success. Several empirical selection methods have been reported, a number of which are summarised in this review. Of notable significance are the ‘global’ methods based on mapping of transcripts with the endoribonuclease H (RNase H) and oligonucleotide scanning arrays.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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