Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases.
Identifieur interne : 003255 ( Main/Merge ); précédent : 003254; suivant : 003256Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases.
Auteurs : Pierre-Alain Monnard [États-Unis] ; Anastassia Kanavarioti ; David W. DeamerSource :
- Journal of the American Chemical Society [ 0002-7863 ] ; 2003.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN, Pancreatic ribonuclease, Purines, Pyrimidines, Ribonuclease T1, Ribonucléotides.
- ARN, Pancreatic ribonuclease, Purines, Pyrimidines, Ribonuclease T1, Ribonucléotides.
English descriptors
- KwdEn :
- Purines (chemistry), Purines (metabolism), Pyrimidines (chemistry), Pyrimidines (metabolism), RNA (chemistry), RNA (metabolism), Ribonuclease T1 (chemistry), Ribonuclease T1 (metabolism), Ribonuclease, Pancreatic (chemistry), Ribonuclease, Pancreatic (metabolism), Ribonucleotides (chemistry), Ribonucleotides (metabolism).
- MESH :
- chemical , chemistry : Purines, Pyrimidines, RNA, Ribonuclease T1, Ribonuclease, Pancreatic, Ribonucleotides.
- chemical , metabolism : Purines, Pyrimidines, RNA, Ribonuclease T1, Ribonuclease, Pancreatic, Ribonucleotides.
Abstract
The RNA world hypothesis requires a plausible mechanism by which RNA itself (or precursor RNA-like polymers) can be synthesized nonenzymatically from the corresponding building blocks. Simulation experiments have exploited chemically reactive mononucleotides as monomers. Solutions of such monomers in the prebiotic environment were likely to be very dilute, but in experimental simulations of polymerization reactions dilute solutions of activated mononucleotides in the millimolar range hydrolyze extensively, and only trace amounts of dimers and trimers are formed. We report here that random medium-size RNA analogues with mixed sequences (5- to 17-mers with traces of longer products) can be synthesized in ice eutectic phases that are produced when dilute solutions of activated monomers and catalysts (Mg(II) and Pb(II)) are frozen and maintained at -18 degrees C for periods up to 38 days. Under these conditions, the monomers are concentrated as eutectics in an ice matrix. Hydrolysis of the activated mononucleotides was suppressed at low-temperature ranges, and polymerization was enhanced with yields up to 90%. Analysis of the mixed oligomers established that incorporation of both purine and pyrimidine bases proceeded at comparable rates and yields. These results suggest that ice deposits on the early Earth could have facilitated the synthesis of short- and medium-size random sequence RNA analogues and thereby provided a microenvironment suitable for the formation of biopolymers or their precursors.
DOI: 10.1021/ja036465h
PubMed: 14599212
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002427
- to stream PubMed, to step Curation: 002427
- to stream PubMed, to step Checkpoint: 002325
- to stream Ncbi, to step Merge: 000242
- to stream Ncbi, to step Curation: 000242
- to stream Ncbi, to step Checkpoint: 000242
Links to Exploration step
pubmed:14599212Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases.</title><author><name sortKey="Monnard, Pierre Alain" sort="Monnard, Pierre Alain" uniqKey="Monnard P" first="Pierre-Alain" last="Monnard">Pierre-Alain Monnard</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Biochemistry, University of California, 1156 High Street, Santa Cruz, California 95064, USA. monnard@molbio.mgh.harvard.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, University of California, 1156 High Street, Santa Cruz, California 95064</wicri:regionArea><wicri:noRegion>California 95064</wicri:noRegion></affiliation></author><author><name sortKey="Kanavarioti, Anastassia" sort="Kanavarioti, Anastassia" uniqKey="Kanavarioti A" first="Anastassia" last="Kanavarioti">Anastassia Kanavarioti</name></author><author><name sortKey="Deamer, David W" sort="Deamer, David W" uniqKey="Deamer D" first="David W" last="Deamer">David W. Deamer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14599212</idno><idno type="pmid">14599212</idno><idno type="doi">10.1021/ja036465h</idno><idno type="wicri:Area/PubMed/Corpus">002427</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002427</idno><idno type="wicri:Area/PubMed/Curation">002427</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002427</idno><idno type="wicri:Area/PubMed/Checkpoint">002325</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002325</idno><idno type="wicri:Area/Ncbi/Merge">000242</idno><idno type="wicri:Area/Ncbi/Curation">000242</idno><idno type="wicri:Area/Ncbi/Checkpoint">000242</idno><idno type="wicri:doubleKey">0002-7863:2003:Monnard P:eutectic:phase:polymerization</idno><idno type="wicri:Area/Main/Merge">003255</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases.</title><author><name sortKey="Monnard, Pierre Alain" sort="Monnard, Pierre Alain" uniqKey="Monnard P" first="Pierre-Alain" last="Monnard">Pierre-Alain Monnard</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Biochemistry, University of California, 1156 High Street, Santa Cruz, California 95064, USA. monnard@molbio.mgh.harvard.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry and Biochemistry, University of California, 1156 High Street, Santa Cruz, California 95064</wicri:regionArea><wicri:noRegion>California 95064</wicri:noRegion></affiliation></author><author><name sortKey="Kanavarioti, Anastassia" sort="Kanavarioti, Anastassia" uniqKey="Kanavarioti A" first="Anastassia" last="Kanavarioti">Anastassia Kanavarioti</name></author><author><name sortKey="Deamer, David W" sort="Deamer, David W" uniqKey="Deamer D" first="David W" last="Deamer">David W. Deamer</name></author></analytic><series><title level="j">Journal of the American Chemical Society</title><idno type="ISSN">0002-7863</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Purines (chemistry)</term><term>Purines (metabolism)</term><term>Pyrimidines (chemistry)</term><term>Pyrimidines (metabolism)</term><term>RNA (chemistry)</term><term>RNA (metabolism)</term><term>Ribonuclease T1 (chemistry)</term><term>Ribonuclease T1 (metabolism)</term><term>Ribonuclease, Pancreatic (chemistry)</term><term>Ribonuclease, Pancreatic (metabolism)</term><term>Ribonucleotides (chemistry)</term><term>Ribonucleotides (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ()</term><term>ARN (métabolisme)</term><term>Pancreatic ribonuclease ()</term><term>Pancreatic ribonuclease (métabolisme)</term><term>Purines ()</term><term>Purines (métabolisme)</term><term>Pyrimidines ()</term><term>Pyrimidines (métabolisme)</term><term>Ribonuclease T1 ()</term><term>Ribonuclease T1 (métabolisme)</term><term>Ribonucléotides ()</term><term>Ribonucléotides (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Purines</term><term>Pyrimidines</term><term>RNA</term><term>Ribonuclease T1</term><term>Ribonuclease, Pancreatic</term><term>Ribonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Purines</term><term>Pyrimidines</term><term>RNA</term><term>Ribonuclease T1</term><term>Ribonuclease, Pancreatic</term><term>Ribonucleotides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN</term><term>Pancreatic ribonuclease</term><term>Purines</term><term>Pyrimidines</term><term>Ribonuclease T1</term><term>Ribonucléotides</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ARN</term><term>Pancreatic ribonuclease</term><term>Purines</term><term>Pyrimidines</term><term>Ribonuclease T1</term><term>Ribonucléotides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The RNA world hypothesis requires a plausible mechanism by which RNA itself (or precursor RNA-like polymers) can be synthesized nonenzymatically from the corresponding building blocks. Simulation experiments have exploited chemically reactive mononucleotides as monomers. Solutions of such monomers in the prebiotic environment were likely to be very dilute, but in experimental simulations of polymerization reactions dilute solutions of activated mononucleotides in the millimolar range hydrolyze extensively, and only trace amounts of dimers and trimers are formed. We report here that random medium-size RNA analogues with mixed sequences (5- to 17-mers with traces of longer products) can be synthesized in ice eutectic phases that are produced when dilute solutions of activated monomers and catalysts (Mg(II) and Pb(II)) are frozen and maintained at -18 degrees C for periods up to 38 days. Under these conditions, the monomers are concentrated as eutectics in an ice matrix. Hydrolysis of the activated mononucleotides was suppressed at low-temperature ranges, and polymerization was enhanced with yields up to 90%. Analysis of the mixed oligomers established that incorporation of both purine and pyrimidine bases proceeded at comparable rates and yields. These results suggest that ice deposits on the early Earth could have facilitated the synthesis of short- and medium-size random sequence RNA analogues and thereby provided a microenvironment suitable for the formation of biopolymers or their precursors.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003255 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003255 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:14599212
|texte= Eutectic phase polymerization of activated ribonucleotide mixtures yields quasi-equimolar incorporation of purine and pyrimidine nucleobases.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:14599212" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |