Detection of Specific Sequences in RNA Using Differential Adsorption of Single-Stranded Oligonucleotides on Gold Nanoparticles
Identifieur interne : 002F72 ( Main/Merge ); précédent : 002F71; suivant : 002F73Detection of Specific Sequences in RNA Using Differential Adsorption of Single-Stranded Oligonucleotides on Gold Nanoparticles
Auteurs : Huixiang Li [États-Unis] ; Lewis Rothberg [États-Unis]Source :
- Analytical Chemistry [ 0003-2700 ] ; 2005.
Abstract
We have used the disparity in adsorption rates for single- and double-stranded RNA on ionically coated gold nanoparticles suspended in a colloid to design a rapid sequence identification assay. Unlabeled target RNA and a probe sequence are mixed prior to exposure to the gold nanoparticles to enable efficient hybridization. We have designed assays based on either color changes or fluorescence that are sensitive to a few picomoles of target. Single-base mutations on RNA sequences can be detected even in complex oligonucleotide mixtures. The assay requires less than 10 min so that RNA degradation problems are avoided.
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DOI: 10.1021/ac050921y
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Differential Adsorption of Single-Stranded
Oligonucleotides on Gold Nanoparticles</title><author><name sortKey="Li, Huixiang" sort="Li, Huixiang" uniqKey="Li H" first="Huixiang" last="Li">Huixiang Li</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Chemistry, University of Rochester, Rochester</wicri:cityArea></affiliation></author><author><name sortKey="Rothberg, Lewis" sort="Rothberg, Lewis" uniqKey="Rothberg L" first="Lewis" last="Rothberg">Lewis Rothberg</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">État de New York</region></placeName><wicri:cityArea>Department of Chemistry, University of Rochester, Rochester</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Analytical Chemistry</title><title level="j" type="abbrev">Anal. Chem.</title><idno type="ISSN">0003-2700</idno><idno type="eISSN">1520-6882</idno><imprint><publisher>American Chemical Society</publisher><date type="e-published">2005</date><date type="published">2005</date><biblScope unit="vol">77</biblScope><biblScope unit="issue">19</biblScope><biblScope unit="page" from="6229">6229</biblScope><biblScope unit="page" to="6233">6233</biblScope></imprint><idno type="ISSN">0003-2700</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0003-2700</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">We have used the disparity in adsorption rates for single- and double-stranded RNA on ionically coated gold nanoparticles suspended in a colloid to design a rapid sequence identification assay. Unlabeled target RNA and a probe sequence are mixed prior to exposure to the gold nanoparticles to enable efficient hybridization. We have designed assays based on either color changes or fluorescence that are sensitive to a few picomoles of target. Single-base mutations on RNA sequences can be detected even in complex oligonucleotide mixtures. The assay requires less than 10 min so that RNA degradation problems are avoided.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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