Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.
Identifieur interne : 001862 ( Main/Merge ); précédent : 001861; suivant : 001863Comparison of serological assays in human Middle East respiratory syndrome (MERS)-coronavirus infection.
Auteurs : Sang Won Park ; Ranawaka A P M. Perera ; Pyoeng Gyun Choe ; Eric H Y. Lau ; Seong Jin Choi ; June Young Chun ; Hong Sang Oh ; Kyoung-Ho Song ; Ji Hwan Bang ; Eu Suk Kim ; Hong Bin Kim ; Wan Beom Park ; Nam Joong Kim ; Leo Lit Man Poon ; Malik Peiris ; Myoung Don OhSource :
- Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin [ 1560-7917 ] ; 2015.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Anticorps antiviraux (sang), Anticorps neutralisants (sang), Chameaux (sang), Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Femelle, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), Mâle, Test ELISA (), Tests de neutralisation ().
- MESH :
- diagnostic : Infections à coronavirus.
- immunologie : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- sang : Anticorps antiviraux, Anticorps neutralisants, Chameaux.
- virologie : Infections à coronavirus.
- Adulte, Animaux, Femelle, Humains, Mâle, Test ELISA, Tests de neutralisation.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Neutralizing (blood), Antibodies, Viral (blood), Camelus (blood), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Male, Middle East Respiratory Syndrome Coronavirus (immunology), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Neutralization Tests (methods).
- MESH :
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- blood : Camelus.
- diagnosis : Coronavirus Infections.
- immunology : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Enzyme-Linked Immunosorbent Assay, Neutralization Tests.
- virology : Coronavirus Infections.
- Adult, Animals, Female, Humans, Male.
Abstract
Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous.
DOI: 10.2807/1560-7917.ES.2015.20.41.30042
PubMed: 26538277
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pubmed:26538277Le document en format XML
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<wicri:noCountry code="no comma">These authors contributed equally to the work.</wicri:noCountry>
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<author><name sortKey="Perera, Ranawaka A P M" sort="Perera, Ranawaka A P M" uniqKey="Perera R" first="Ranawaka A P M" last="Perera">Ranawaka A P M. Perera</name>
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<term>Antibodies, Neutralizing (blood)</term>
<term>Antibodies, Viral (blood)</term>
<term>Camelus (blood)</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (virology)</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Female</term>
<term>Humans</term>
<term>Male</term>
<term>Middle East Respiratory Syndrome Coronavirus (immunology)</term>
<term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term>
<term>Neutralization Tests (methods)</term>
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<term>Animaux</term>
<term>Anticorps antiviraux (sang)</term>
<term>Anticorps neutralisants (sang)</term>
<term>Chameaux (sang)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (immunologie)</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term>
<term>Femelle</term>
<term>Humains</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Mâle</term>
<term>Test ELISA ()</term>
<term>Tests de neutralisation ()</term>
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<term>Antibodies, Viral</term>
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</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
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<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term>
<term>Neutralization Tests</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps neutralisants</term>
<term>Chameaux</term>
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<term>Humans</term>
<term>Male</term>
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<term>Animaux</term>
<term>Femelle</term>
<term>Humains</term>
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<front><div type="abstract" xml:lang="en">Plaque reduction neutralisation tests (PRNT), microneutralisation (MN), Middle East respiratory syndrome (MERS)-spike pseudoparticle neutralisation (ppNT) and MERS S1-enzyme-linked immunosorbent assay (ELISA) antibody titres were compared using 95 sera from 17 patients with MERS, collected two to 46 days after symptom onset. Neutralisation tests correlated well with each other and moderately well with S1 ELISA. Moreover to compare antigenic similarity of genetically diverse MERS-CoV clades, the response of four sera from two patients sampled at two time periods during the course of illness were tested by 90% PRNT. Genetically diverse MERS-CoV clades were antigenically homogenous. </div>
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