Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome
Identifieur interne : 001603 ( Main/Merge ); précédent : 001602; suivant : 001604Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome
Auteurs : Christina E. Douglas ; David A. Kulesh ; James G. Jaissle ; Timothy D. MinogueSource :
- Molecular and Cellular Probes [ 0890-8508 ] ; 2015.
Descripteurs français
- KwdFr :
- ARN viral (analyse), Amorces ADN (analyse), Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (virologie), RT-PCR (), Réaction de polymérisation en chaine en temps réel (), Sensibilité et spécificité, Sondes d'ADN (analyse), Techniques de diagnostic moléculaire ().
- MESH :
- analyse : ARN viral, Amorces ADN, Sondes d'ADN.
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus.
- isolement et purification : Coronavirus.
- virologie : Infections à coronavirus.
- Coronavirus, Humains, RT-PCR, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus Infections (diagnosis), Coronavirus Infections (virology), DNA Primers (analysis), DNA Probes (analysis), Humans, Molecular Diagnostic Techniques (methods), RNA, Viral (analysis), Real-Time Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (methods), Sensitivity and Specificity.
- MESH :
- chemical , analysis : DNA Primers, DNA Probes, RNA, Viral.
- classification : Coronavirus.
- diagnosis : Coronavirus Infections.
- genetics : Coronavirus.
- isolation & purification : Coronavirus.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction.
- virology : Coronavirus Infections.
- Humans, Sensitivity and Specificity.
Abstract
Here we designed and tested two highly specific quantitative TaqMan®-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).
Url:
DOI: 10.1016/j.mcp.2015.09.001
PubMed: 26365228
PubMed Central: 7126732
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PMC:7126732Le document en format XML
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Douglas</name></author><author><name sortKey="Kulesh, David A" sort="Kulesh, David A" uniqKey="Kulesh D" first="David A." last="Kulesh">David A. Kulesh</name></author><author><name sortKey="Jaissle, James G" sort="Jaissle, James G" uniqKey="Jaissle J" first="James G." last="Jaissle">James G. Jaissle</name></author><author><name sortKey="Minogue, Timothy D" sort="Minogue, Timothy D" uniqKey="Minogue T" first="Timothy D." last="Minogue">Timothy D. Minogue</name></author></analytic><series><title level="j">Molecular and Cellular Probes</title><idno type="ISSN">0890-8508</idno><idno type="eISSN">1096-1194</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus (classification)</term><term>Coronavirus (genetics)</term><term>Coronavirus (isolation & purification)</term><term>Coronavirus Infections (diagnosis)</term><term>Coronavirus Infections (virology)</term><term>DNA Primers (analysis)</term><term>DNA Probes (analysis)</term><term>Humans</term><term>Molecular Diagnostic Techniques (methods)</term><term>RNA, Viral (analysis)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>Amorces ADN (analyse)</term><term>Coronavirus ()</term><term>Coronavirus (génétique)</term><term>Coronavirus (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Infections à coronavirus (virologie)</term><term>RT-PCR ()</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Sensibilité et spécificité</term><term>Sondes d'ADN (analyse)</term><term>Techniques de diagnostic moléculaire ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA Primers</term><term>DNA Probes</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term><term>Amorces ADN</term><term>Sondes d'ADN</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Real-Time Polymerase Chain Reaction</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Coronavirus</term><term>Humains</term><term>RT-PCR</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Sensibilité et spécificité</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Here we designed and tested two highly specific quantitative TaqMan<sup>®</sup>-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Al Abdallat, M M" uniqKey="Al Abdallat M">M.M. Al-Abdallat</name></author><author><name sortKey="Payne, D C" uniqKey="Payne D">D.C. Payne</name></author><author><name sortKey="Alqasrawi, S" uniqKey="Alqasrawi S">S. Alqasrawi</name></author><author><name sortKey="Rha, B" uniqKey="Rha B">B. Rha</name></author><author><name sortKey="Tohme, R A" uniqKey="Tohme R">R.A. Tohme</name></author><author><name sortKey="Abedi, G R" uniqKey="Abedi G">G.R. Abedi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gralinski, L E" uniqKey="Gralinski L">L.E. Gralinski</name></author><author><name sortKey="Baric, R S" uniqKey="Baric R">R.S. Baric</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Eckerle, I" uniqKey="Eckerle I">I. Eckerle</name></author><author><name sortKey="Bleicker, T" uniqKey="Bleicker T">T. Bleicker</name></author><author><name sortKey="Zaki, A" uniqKey="Zaki A">A. Zaki</name></author><author><name sortKey="Landt, O" uniqKey="Landt O">O. Landt</name></author><author><name sortKey="Eschbach Bludau, M" uniqKey="Eschbach Bludau M">M. Eschbach-Bludau</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, X" uniqKey="Lu X">X. Lu</name></author><author><name sortKey="Whitaker, B" uniqKey="Whitaker B">B. Whitaker</name></author><author><name sortKey="Sakthivel, S K" uniqKey="Sakthivel S">S.K. Sakthivel</name></author><author><name sortKey="Kamili, S" uniqKey="Kamili S">S. Kamili</name></author><author><name sortKey="Rose, L E" uniqKey="Rose L">L.E. Rose</name></author><author><name sortKey="Lowe, L" uniqKey="Lowe L">L. Lowe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Corman, V M" uniqKey="Corman V">V.M. Corman</name></author><author><name sortKey="Muller, M A" uniqKey="Muller M">M.A. Muller</name></author><author><name sortKey="Costabel, U" uniqKey="Costabel U">U. Costabel</name></author><author><name sortKey="Timm, J" uniqKey="Timm J">J. Timm</name></author><author><name sortKey="Binger, T" uniqKey="Binger T">T. Binger</name></author><author><name sortKey="Meyer, B" uniqKey="Meyer B">B. Meyer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Cotten, M" uniqKey="Cotten M">M. Cotten</name></author><author><name sortKey="Watson, S J" uniqKey="Watson S">S.J. Watson</name></author><author><name sortKey="Zumla, A I" uniqKey="Zumla A">A.I. Zumla</name></author><author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name></author><author><name sortKey="Palser, A L" uniqKey="Palser A">A.L. Palser</name></author><author><name sortKey="Ong, S H" uniqKey="Ong S">S.H. Ong</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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