Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry
Identifieur interne : 000E03 ( Main/Merge ); précédent : 000E02; suivant : 000E04Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry
Auteurs : Leshan Xiu ; Chi Zhang ; Zhiqiang Wu ; Junping PengSource :
- Frontiers in Microbiology [ 1664-302X ] ; 2017.
Abstract
There are four human coronaviruses (HCoVs), distributed worldwide, that are associated with a range of respiratory symptoms. The discovery of severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV shows that HCoVs pose a significant threat to human health. Our work aims to develop a sensitive method (mCoV-MS) which can not only identify known HCoVs accurately, but also have the ability to provide clues for the emerging HCoVs. The method was performed using a MassARRAY matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. We developed a 17-plex analysis to detect six HCoVs in Panel A and another 17-plex analysis to detect
Url:
DOI: 10.3389/fmicb.2017.01510
PubMed: 28848521
PubMed Central: 5552709
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Establishment and Application of a Universal Coronavirus Screening Method Using MALDI-TOF Mass Spectrometry</title>
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<author><name sortKey="Zhang, Chi" sort="Zhang, Chi" uniqKey="Zhang C" first="Chi" last="Zhang">Chi Zhang</name>
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<front><div type="abstract" xml:lang="en"><p>There are four human coronaviruses (HCoVs), distributed worldwide, that are associated with a range of respiratory symptoms. The discovery of severe acute respiratory syndrome (SARS)-CoV and Middle East respiratory syndrome (MERS)-CoV shows that HCoVs pose a significant threat to human health. Our work aims to develop a sensitive method (mCoV-MS) which can not only identify known HCoVs accurately, but also have the ability to provide clues for the emerging HCoVs. The method was performed using a MassARRAY matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system. We developed a 17-plex analysis to detect six HCoVs in Panel A and another 17-plex analysis to detect <italic>Alphacoronavirus</italic>
and <italic>Betacoronavirus</italic>
in Panel B. All tested primers and probes for the mCoV-MS method were effective, with no cross-reactivity observed with other common respiratory viruses. To confirm the usefulness of the mCoV-MS method we screened 384 pharyngeal and/or anal swab samples collected from bats/rodents, and 131 nasal and throat swabs from human patients. The results showed good concordance with the results of metagenomic analysis or PCR-sequencing. The validation test showed mCoV-MS method can detect potentially pathogenic CoVs in <italic>Alphacoronavirus</italic>
and <italic>Betacoronavirus</italic>
and provide convincingly phylogenetic evidences about unknown CoVs. The mCoV-MS method is a sensitive assay that is relatively simple to carry out. We propose that this method be used to complement next generation sequencing technology for large-scale screening studies.</p>
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