MersV1, Main, Exploration, bibRecordById, pubmed:8670082

Selection of hammerhead ribozymes for optimum cleavage of interleukin 6 mRNA.

Identifieur interne : 003E11 ( Main/Exploration ); précédent : 003E10; suivant : 003E12

Selection of hammerhead ribozymes for optimum cleavage of interleukin 6 mRNA.

Auteurs : C. Hendrix [Belgique] ; J. Anné ; B. Joris ; A. Van Aerschot ; P. Herdewijn

Source :

The Biochemical journal [ 0264-6021 ] ; 1996.

RBID : pubmed:8670082

Descripteurs français

KwdFr :
- ARN catalytique (), ARN catalytique (métabolisme), ARN messager (métabolisme), Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Interleukine-6 (génétique), Spectrophotométrie UV, Spécificité du substrat, Séquence nucléotidique, Température.
MESH :
- génétique : Interleukine-6.
- métabolisme : ARN catalytique, ARN messager.
- ARN catalytique, Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Spectrophotométrie UV, Spécificité du substrat, Séquence nucléotidique, Température.

English descriptors

KwdEn :
- Base Sequence, Interleukin-6 (genetics), Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Catalytic (chemistry), RNA, Catalytic (metabolism), RNA, Messenger (metabolism), Spectrophotometry, Ultraviolet, Substrate Specificity, Temperature.
MESH :
- chemical , chemistry : RNA, Catalytic.
- chemical , genetics : Interleukin-6.
- chemical , metabolism : RNA, Catalytic, RNA, Messenger.
- Base Sequence, Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, Spectrophotometry, Ultraviolet, Substrate Specificity, Temperature.

Abstract

Four GUC triplets in the coding region of the MRNA of interleukin 6 (IL-6) were examined for their suitabilty to serve as a target for hammerhead ribozome-mediated cleavage. This selection procedure was performed with the intention to downregulate IL-6 production as a potential treatment of those diseases in which IL-6 overexpression is involved. Hammerhead ribozymes and their respective short synthetic substrates (19-mers) were synthesized for these four GUC triplets. Notwithstanding the identical catalytic core sequences, the difference in base composition of the helices involved in substrate binding caused substantial variation in cleavage activity. The cleavage reactions on the 1035 nucleotide IL-6 mRNA transcript revealed that two ribozymes were able to cleave this substrate, showing a decrease in catalytic efficiency to 1/30 and 1/300 of the short substrate. This study indicates that the GUC triplet located at nucleotide 510 of the mRNA of IL-6 is the best site for hammerhead ribozyme-mediated cleavage. We suggest that in future targeting of chemically modified hammerhead ribosomes for cleavage of IL-6 RNA should be directed at this location.

DOI: 10.1042/bj3140655
PubMed: 8670082

Affiliations:

Belgique

Le document en format XML

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<term>Nucleic Acid Conformation</term>
<term>RNA, Catalytic (chemistry)</term>
<term>RNA, Catalytic (metabolism)</term>
<term>RNA, Messenger (metabolism)</term>
<term>Spectrophotometry, Ultraviolet</term>
<term>Substrate Specificity</term>
<term>Temperature</term>
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<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Interleukine-6 (génétique)</term>
<term>Spectrophotométrie UV</term>
<term>Spécificité du substrat</term>
<term>Séquence nucléotidique</term>
<term>Température</term>
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<term>Kinetics</term>
<term>Molecular Sequence Data</term>
<term>Nucleic Acid Conformation</term>
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<term>Substrate Specificity</term>
<term>Temperature</term>
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<term>Cinétique</term>
<term>Conformation d'acide nucléique</term>
<term>Données de séquences moléculaires</term>
<term>Spectrophotométrie UV</term>
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<term>Séquence nucléotidique</term>
<term>Température</term>
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<front><div type="abstract" xml:lang="en">Four GUC triplets in the coding region of the MRNA of interleukin 6 (IL-6) were examined for their suitabilty to serve as a target for hammerhead ribozome-mediated cleavage. This selection procedure was performed with the intention to downregulate IL-6 production as a potential treatment of those diseases in which IL-6 overexpression is involved. Hammerhead ribozymes and their respective short synthetic substrates (19-mers) were synthesized for these four GUC triplets. Notwithstanding the identical catalytic core sequences, the difference in base composition of the helices involved in substrate binding caused substantial variation in cleavage activity. The cleavage reactions on the 1035 nucleotide IL-6 mRNA transcript revealed that two ribozymes were able to cleave this substrate, showing a decrease in catalytic efficiency to 1/30 and 1/300 of the short substrate. This study indicates that the GUC triplet located at nucleotide 510 of the mRNA of IL-6 is the best site for hammerhead ribozyme-mediated cleavage. We suggest that in future targeting of chemically modified hammerhead ribosomes for cleavage of IL-6 RNA should be directed at this location.</div>
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<name sortKey="Joris, B" sort="Joris, B" uniqKey="Joris B" first="B" last="Joris">B. Joris</name>
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Serveur d'exploration MERS

Selection of hammerhead ribozymes for optimum cleavage of interleukin 6 mRNA.

Selection of hammerhead ribozymes for optimum cleavage of interleukin 6 mRNA.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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Pour générer des pages wiki

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