Two DR beta allelic series defined by exon II-specific synthetic oligonucleotide genomic hybridization: a method of HLA typing?
Identifieur interne : 004D26 ( Main/Exploration ); précédent : 004D25; suivant : 004D27Two DR beta allelic series defined by exon II-specific synthetic oligonucleotide genomic hybridization: a method of HLA typing?
Auteurs : I. Le Gall ; P. Millasseau ; J. Dausset ; D. CohenSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 1986.
Descripteurs français
- KwdFr :
- MESH :
- analyse : Oligonucléotides.
- génétique : Antigènes HLA-D, Antigènes HLA-DR.
- immunologie : Antigènes HLA-DR.
- Allèles, Exons, Hybridation d'acides nucléiques, Polymorphisme génétique, Séquence d'acides aminés, Test d'histocompatibilité.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Oligonucleotides.
- chemical , genetics : HLA-D Antigens, HLA-DR Antigens.
- chemical , immunology : HLA-DR Antigens.
- Alleles, Amino Acid Sequence, Exons, Histocompatibility Testing, Nucleic Acid Hybridization, Polymorphism, Genetic.
Abstract
Comparisons of exon II HLA-DR beta sequences have shown that nucleotide variations are principally clustered within the following three regions: V1 (amino acid 8-15), V2 (25-32), and V3 (70-77). V1, V2, and V3-derived 24-mers have been synthesized, the DR beta sequences coming from DR1, DR3, Drw6, DR4, DR5, and DRw53 haplotypes. Each oligonucleotide was hybridized to Pvu II-digested DNA samples from 13 HLA genotyped families; therefore, 52 haplotypes have been investigated. Six polymorphic Pvu II fragments were detected, constituting two allelic series probably corresponding to the beta 1 and beta 2 locus of the DR region. The first series (beta 1) comprises a minimum of nine alleles while the second series (beta 2), which is less polymorphic, comprises at least four alleles. Certain patterns correlate perfectly with certain DR specificities, whereas other patterns define new subdivisions as in DR3 and DRw6 haplotypes. Although it appears that some mismatches do not always prevent hybridization in the conditions used in this work, this method will provide in many instances a convenient tool for HLA-DR typing.
DOI: 10.1073/pnas.83.20.7836
PubMed: 3464000
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A81
- to stream PubMed, to step Curation: 002A81
- to stream PubMed, to step Checkpoint: 002923
- to stream Ncbi, to step Merge: 002843
- to stream Ncbi, to step Curation: 002843
- to stream Ncbi, to step Checkpoint: 002843
- to stream Main, to step Merge: 004E06
- to stream Main, to step Curation: 004D26
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Two DR beta allelic series defined by exon II-specific synthetic oligonucleotide genomic hybridization: a method of HLA typing?</title><author><name sortKey="Le Gall, I" sort="Le Gall, I" uniqKey="Le Gall I" first="I" last="Le Gall">I. Le Gall</name></author><author><name sortKey="Millasseau, P" sort="Millasseau, P" uniqKey="Millasseau P" first="P" last="Millasseau">P. Millasseau</name></author><author><name sortKey="Dausset, J" sort="Dausset, J" uniqKey="Dausset J" first="J" last="Dausset">J. Dausset</name></author><author><name sortKey="Cohen, D" sort="Cohen, D" uniqKey="Cohen D" first="D" last="Cohen">D. Cohen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1986">1986</date><idno type="RBID">pubmed:3464000</idno><idno type="pmid">3464000</idno><idno type="doi">10.1073/pnas.83.20.7836</idno><idno type="wicri:Area/PubMed/Corpus">002A81</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A81</idno><idno type="wicri:Area/PubMed/Curation">002A81</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A81</idno><idno type="wicri:Area/PubMed/Checkpoint">002923</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002923</idno><idno type="wicri:Area/Ncbi/Merge">002843</idno><idno type="wicri:Area/Ncbi/Curation">002843</idno><idno type="wicri:Area/Ncbi/Checkpoint">002843</idno><idno type="wicri:doubleKey">0027-8424:1986:Le Gall I:two:dr:beta</idno><idno type="wicri:Area/Main/Merge">004E06</idno><idno type="wicri:Area/Main/Curation">004D26</idno><idno type="wicri:Area/Main/Exploration">004D26</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Two DR beta allelic series defined by exon II-specific synthetic oligonucleotide genomic hybridization: a method of HLA typing?</title><author><name sortKey="Le Gall, I" sort="Le Gall, I" uniqKey="Le Gall I" first="I" last="Le Gall">I. Le Gall</name></author><author><name sortKey="Millasseau, P" sort="Millasseau, P" uniqKey="Millasseau P" first="P" last="Millasseau">P. Millasseau</name></author><author><name sortKey="Dausset, J" sort="Dausset, J" uniqKey="Dausset J" first="J" last="Dausset">J. Dausset</name></author><author><name sortKey="Cohen, D" sort="Cohen, D" uniqKey="Cohen D" first="D" last="Cohen">D. Cohen</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="1986" type="published">1986</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alleles</term><term>Amino Acid Sequence</term><term>Exons</term><term>HLA-D Antigens (genetics)</term><term>HLA-DR Antigens (genetics)</term><term>HLA-DR Antigens (immunology)</term><term>Histocompatibility Testing</term><term>Nucleic Acid Hybridization</term><term>Oligonucleotides (analysis)</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Allèles</term><term>Antigènes HLA-D (génétique)</term><term>Antigènes HLA-DR (génétique)</term><term>Antigènes HLA-DR (immunologie)</term><term>Exons</term><term>Hybridation d'acides nucléiques</term><term>Oligonucléotides (analyse)</term><term>Polymorphisme génétique</term><term>Séquence d'acides aminés</term><term>Test d'histocompatibilité</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>HLA-D Antigens</term><term>HLA-DR Antigens</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>HLA-DR Antigens</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Oligonucléotides</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes HLA-D</term><term>Antigènes HLA-DR</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes HLA-DR</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Alleles</term><term>Amino Acid Sequence</term><term>Exons</term><term>Histocompatibility Testing</term><term>Nucleic Acid Hybridization</term><term>Polymorphism, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Allèles</term><term>Exons</term><term>Hybridation d'acides nucléiques</term><term>Polymorphisme génétique</term><term>Séquence d'acides aminés</term><term>Test d'histocompatibilité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Comparisons of exon II HLA-DR beta sequences have shown that nucleotide variations are principally clustered within the following three regions: V1 (amino acid 8-15), V2 (25-32), and V3 (70-77). V1, V2, and V3-derived 24-mers have been synthesized, the DR beta sequences coming from DR1, DR3, Drw6, DR4, DR5, and DRw53 haplotypes. Each oligonucleotide was hybridized to Pvu II-digested DNA samples from 13 HLA genotyped families; therefore, 52 haplotypes have been investigated. Six polymorphic Pvu II fragments were detected, constituting two allelic series probably corresponding to the beta 1 and beta 2 locus of the DR region. The first series (beta 1) comprises a minimum of nine alleles while the second series (beta 2), which is less polymorphic, comprises at least four alleles. Certain patterns correlate perfectly with certain DR specificities, whereas other patterns define new subdivisions as in DR3 and DRw6 haplotypes. Although it appears that some mismatches do not always prevent hybridization in the conditions used in this work, this method will provide in many instances a convenient tool for HLA-DR typing.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Cohen, D" sort="Cohen, D" uniqKey="Cohen D" first="D" last="Cohen">D. Cohen</name><name sortKey="Dausset, J" sort="Dausset, J" uniqKey="Dausset J" first="J" last="Dausset">J. Dausset</name><name sortKey="Le Gall, I" sort="Le Gall, I" uniqKey="Le Gall I" first="I" last="Le Gall">I. Le Gall</name><name sortKey="Millasseau, P" sort="Millasseau, P" uniqKey="Millasseau P" first="P" last="Millasseau">P. Millasseau</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004D26 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004D26 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:3464000
|texte= Two DR beta allelic series defined by exon II-specific synthetic oligonucleotide genomic hybridization: a method of HLA typing?
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:3464000" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |