MERS-CoV diagnosis: An update
Identifieur interne : 001168 ( Main/Exploration ); précédent : 001167; suivant : 001169MERS-CoV diagnosis: An update
Auteurs : Sameera Al Johani ; Ali H. HajeerSource :
- Journal of Infection and Public Health [ 1876-0341 ] ; 2016.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (), Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Humains, Infections à coronavirus (diagnostic), Réaction de polymérisation en chaine en temps réel (), Techniques de diagnostic moléculaire (), Tests sérologiques ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Coronavirus du syndrome respiratoire du Moyen-Orient.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- Analyse de séquence d'ADN, Humains, Réaction de polymérisation en chaine en temps réel, Techniques de diagnostic moléculaire, Tests sérologiques.
English descriptors
- KwdEn :
- Coronavirus Infections (diagnosis), Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Real-Time Polymerase Chain Reaction (methods), Sequence Analysis, DNA (methods), Serologic Tests (methods).
- MESH :
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques, Real-Time Polymerase Chain Reaction, Sequence Analysis, DNA, Serologic Tests.
- Humans.
Abstract
Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.
Url:
DOI: 10.1016/j.jiph.2016.04.005
PubMed: 27106390
PubMed Central: 7102781
Affiliations:
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Hajeer</name></author></analytic><series><title level="j">Journal of Infection and Public Health</title><idno type="ISSN">1876-0341</idno><idno type="eISSN">1876-035X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus Infections (diagnosis)</term><term>Humans</term><term>Middle East Respiratory Syndrome Coronavirus (genetics)</term><term>Middle East Respiratory Syndrome Coronavirus (isolation & purification)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Real-Time Polymerase Chain Reaction (methods)</term><term>Sequence Analysis, DNA (methods)</term><term>Serologic Tests (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN ()</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (génétique)</term><term>Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification)</term><term>Humains</term><term>Infections à coronavirus (diagnostic)</term><term>Réaction de polymérisation en chaine en temps réel ()</term><term>Techniques de diagnostic moléculaire ()</term><term>Tests sérologiques ()</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term><term>Real-Time Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Humains</term><term>Réaction de polymérisation en chaine en temps réel</term><term>Techniques de diagnostic moléculaire</term><term>Tests sérologiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Summary</title><p>Diagnosis of MERS-Cov still a major concern in most of daignostic laboratories. To date the Real-time Polymerase Chain reaction (RT-PCR) is the mainstay for diagnosis of MERS-CoV. RT-PCR has limitations, including a long turnaround time and lack of common measurements and correlations with Viral Load (VL). It is recommended to screen for MERS-CoV using RT-PCR of the upstream of envelope gene (upE) followed by confirmation of the presence of one of the following genes; open reading frame 1A, 1B genes or nucleocapsid (N) gene. Scientists are looking to implement viral sequencing on all negative samples by RT-PCR and they beleive that can be exposed to another level of testing using sequencing of the RNA-dependent RNA polymerase (RdRp) gene or N gene and in this case a positive result is diagnostic. It is also very important to maintain a contineous and random sequencing for MERS-Cov samples to be able to pick early viral mutations. Serological assays still not widely or routinely performed, and a lot of studies looking to implement such method in routine patient's testings.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Memish, Z A" uniqKey="Memish Z">Z.A. Memish</name></author><author><name sortKey="Al Tawfiq, J A" uniqKey="Al Tawfiq J">J.A. Al-Tawfiq</name></author><author><name sortKey="Makhdoom, H Q" uniqKey="Makhdoom H">H.Q. Makhdoom</name></author><author><name sortKey="Assiri, A" uniqKey="Assiri A">A. Assiri</name></author><author><name sortKey="Alhakeem, R F" uniqKey="Alhakeem R">R.F. Alhakeem</name></author><author><name sortKey="Albarrak, A" uniqKey="Albarrak A">A. Albarrak</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guery, B" uniqKey="Guery B">B. Guery</name></author><author><name sortKey="Poissy, J" uniqKey="Poissy J">J. 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Turkestani</name></author><author><name sortKey="Ritz, D" uniqKey="Ritz D">D. Ritz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, Y D" uniqKey="Wang Y">Y.D. Wang</name></author><author><name sortKey="Li, Y" uniqKey="Li Y">Y. Li</name></author><author><name sortKey="Xu, G B" uniqKey="Xu G">G.B. Xu</name></author><author><name sortKey="Dong, X Y" uniqKey="Dong X">X.Y. Dong</name></author><author><name sortKey="Yang, X A" uniqKey="Yang X">X.A. Yang</name></author><author><name sortKey="Feng, Z R" uniqKey="Feng Z">Z.R. Feng</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Al Johani, Sameera" sort="Al Johani, Sameera" uniqKey="Al Johani S" first="Sameera" last="Al Johani">Sameera Al Johani</name><name sortKey="Hajeer, Ali H" sort="Hajeer, Ali H" uniqKey="Hajeer A" first="Ali H." last="Hajeer">Ali H. 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