Epitope mapping of snake venom phospholipases A2 with pseudexin monoclonal antibodies.
Identifieur interne : 004881 ( Main/Exploration ); précédent : 004880; suivant : 004882Epitope mapping of snake venom phospholipases A2 with pseudexin monoclonal antibodies.
Auteurs : B G Stiles [États-Unis] ; J L MiddlebrookSource :
- Journal of protein chemistry [ 0277-8033 ] ; 1991.
Descripteurs français
- KwdFr :
- Animaux, Anticorps monoclonaux, Calcium (métabolisme), Données de séquences moléculaires, Fixation compétitive, Phospholipases A (), Phospholipases A (immunologie), Phospholipases A2, Simulation numérique, Sites de fixation des anticorps, Souris, Souris de lignée BALB C, Structure moléculaire, Suidae, Séquence d'acides aminés, Test ELISA, Venins de serpent (), Venins des élapidés (), Venins des élapidés (immunologie), Épitopes (), Épitopes (analyse), Épitopes (immunologie).
- MESH :
- analyse : Épitopes.
- immunologie : Phospholipases A, Venins des élapidés, Épitopes.
- métabolisme : Calcium.
- Animaux, Anticorps monoclonaux, Données de séquences moléculaires, Fixation compétitive, Phospholipases A, Phospholipases A2, Simulation numérique, Sites de fixation des anticorps, Souris, Souris de lignée BALB C, Structure moléculaire, Suidae, Séquence d'acides aminés, Test ELISA, Venins de serpent, Venins des élapidés, Épitopes.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal, Binding Sites, Antibody, Binding, Competitive, Calcium (metabolism), Computer Simulation, Elapid Venoms (chemistry), Elapid Venoms (immunology), Enzyme-Linked Immunosorbent Assay, Epitopes (analysis), Epitopes (chemistry), Epitopes (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Structure, Phospholipases A (chemistry), Phospholipases A (immunology), Phospholipases A2, Snake Venoms (chemistry), Swine.
- MESH :
- chemical , analysis : Epitopes.
- chemical , chemistry : Elapid Venoms, Epitopes, Phospholipases A, Snake Venoms.
- chemical , immunology : Elapid Venoms, Epitopes, Phospholipases A.
- chemical , metabolism : Calcium.
- chemical : Antibodies, Monoclonal, Phospholipases A2.
- Amino Acid Sequence, Animals, Binding Sites, Antibody, Binding, Competitive, Computer Simulation, Enzyme-Linked Immunosorbent Assay, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Molecular Structure, Swine.
Abstract
Fifteen different monoclonal antibodies, developed against a pseudexin A, B, and C mixture, were screened for linear epitope recognition. Peptides (9-mers) spanning pseudexin B were synthesized on alanine-derivatized polyethylene pins and subsequently probed with antibody. Four antibodies recognized linear epitopes of pseudexin A, pseudexin B, and also nonidentical sequences found in other phospholipases A2 (PLA2S) as determined by enzyme-linked immunosorbent assays. Three antibodies recognized a highly conserved site important in calcium binding and the interlocking of dimeric forms of PLA2. Antibodies neutralizing lethal or enzymatic effects of PLA2 did not recognize linear epitopes.
DOI: 10.1007/bf01024784
PubMed: 1718309
Affiliations:
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xml:lang="en">Epitope mapping of snake venom phospholipases A2 with pseudexin monoclonal antibodies.</title><author><name sortKey="Stiles, B G" sort="Stiles, B G" uniqKey="Stiles B" first="B G" last="Stiles">B G Stiles</name><affiliation wicri:level="2"><nlm:affiliation>Department of Toxinology, U.S. Army Medical Research Institute of Infectious Diseases, Frederick, Maryland 21702-5011.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Maryland</region></placeName><wicri:cityArea>Department of Toxinology, U.S. Army Medical Research Institute of Infectious Diseases, Frederick</wicri:cityArea></affiliation></author><author><name sortKey="Middlebrook, J L" sort="Middlebrook, J L" uniqKey="Middlebrook J" first="J L" last="Middlebrook">J L Middlebrook</name></author></analytic><series><title level="j">Journal of protein chemistry</title><idno type="ISSN">0277-8033</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal</term><term>Binding Sites, Antibody</term><term>Binding, Competitive</term><term>Calcium (metabolism)</term><term>Computer Simulation</term><term>Elapid Venoms (chemistry)</term><term>Elapid Venoms (immunology)</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Epitopes (analysis)</term><term>Epitopes (chemistry)</term><term>Epitopes (immunology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Phospholipases A (chemistry)</term><term>Phospholipases A (immunology)</term><term>Phospholipases A2</term><term>Snake Venoms (chemistry)</term><term>Swine</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Calcium (métabolisme)</term><term>Données de séquences moléculaires</term><term>Fixation compétitive</term><term>Phospholipases A ()</term><term>Phospholipases A (immunologie)</term><term>Phospholipases A2</term><term>Simulation numérique</term><term>Sites de fixation des anticorps</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure moléculaire</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Test ELISA</term><term>Venins de serpent ()</term><term>Venins des élapidés ()</term><term>Venins des élapidés (immunologie)</term><term>Épitopes ()</term><term>Épitopes (analyse)</term><term>Épitopes (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Epitopes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Elapid Venoms</term><term>Epitopes</term><term>Phospholipases A</term><term>Snake Venoms</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Elapid Venoms</term><term>Epitopes</term><term>Phospholipases A</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Phospholipases A2</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Phospholipases A</term><term>Venins des élapidés</term><term>Épitopes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Calcium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Binding Sites, Antibody</term><term>Binding, Competitive</term><term>Computer Simulation</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Molecular Structure</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Anticorps monoclonaux</term><term>Données de séquences moléculaires</term><term>Fixation compétitive</term><term>Phospholipases A</term><term>Phospholipases A2</term><term>Simulation numérique</term><term>Sites de fixation des anticorps</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure moléculaire</term><term>Suidae</term><term>Séquence d'acides aminés</term><term>Test ELISA</term><term>Venins de serpent</term><term>Venins des élapidés</term><term>Épitopes</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fifteen different monoclonal antibodies, developed against a pseudexin A, B, and C mixture, were screened for linear epitope recognition. Peptides (9-mers) spanning pseudexin B were synthesized on alanine-derivatized polyethylene pins and subsequently probed with antibody. Four antibodies recognized linear epitopes of pseudexin A, pseudexin B, and also nonidentical sequences found in other phospholipases A2 (PLA2S) as determined by enzyme-linked immunosorbent assays. Three antibodies recognized a highly conserved site important in calcium binding and the interlocking of dimeric forms of PLA2. Antibodies neutralizing lethal or enzymatic effects of PLA2 did not recognize linear epitopes.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Maryland</li></region></list><tree><noCountry><name sortKey="Middlebrook, J L" sort="Middlebrook, J L" uniqKey="Middlebrook J" first="J L" last="Middlebrook">J L Middlebrook</name></noCountry><country name="États-Unis"><region name="Maryland"><name sortKey="Stiles, B G" sort="Stiles, B G" uniqKey="Stiles B" first="B G" last="Stiles">B G Stiles</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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