Peptide 15-mers of defined sequence that substitute for random amino acid copolymers in amelioration of experimental autoimmune encephalomyelitis.
Identifieur interne : 002E91 ( Main/Exploration ); précédent : 002E90; suivant : 002E92Peptide 15-mers of defined sequence that substitute for random amino acid copolymers in amelioration of experimental autoimmune encephalomyelitis.
Auteurs : Joel N H. Stern [États-Unis] ; Zsolt Illés ; Jayagopala Reddy ; Derin B. Keskin ; Masha Fridkis-Hareli ; Vijay K. Kuchroo ; Jack L. StromingerSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Conception de médicament, Données de séquences moléculaires, Encéphalomyélite auto-immune expérimentale (immunologie), Encéphalomyélite auto-immune expérimentale (traitement médicamenteux), Lignée cellulaire, Lymphocytes T (), Lymphocytes T (immunologie), Peptides (), Peptides (pharmacologie), Peptides (synthèse chimique), Peptides (usage thérapeutique), Souris, Séquence d'acides aminés, Transfert adoptif.
- MESH :
- immunologie : Encéphalomyélite auto-immune expérimentale, Lymphocytes T.
- pharmacologie : Peptides.
- synthèse chimique : Peptides.
- traitement médicamenteux : Encéphalomyélite auto-immune expérimentale.
- usage thérapeutique : Peptides.
- Animaux, Conception de médicament, Données de séquences moléculaires, Lignée cellulaire, Lymphocytes T, Peptides, Souris, Séquence d'acides aminés, Transfert adoptif.
English descriptors
- KwdEn :
- Adoptive Transfer, Amino Acid Sequence, Animals, Cell Line, Drug Design, Encephalomyelitis, Autoimmune, Experimental (drug therapy), Encephalomyelitis, Autoimmune, Experimental (immunology), Mice, Molecular Sequence Data, Peptides (chemical synthesis), Peptides (chemistry), Peptides (pharmacology), Peptides (therapeutic use), T-Lymphocytes (drug effects), T-Lymphocytes (immunology).
- MESH :
- chemical , chemical synthesis : Peptides.
- chemical , chemistry : Peptides.
- drug effects : T-Lymphocytes.
- drug therapy : Encephalomyelitis, Autoimmune, Experimental.
- immunology : Encephalomyelitis, Autoimmune, Experimental, T-Lymphocytes.
- chemical , pharmacology : Peptides.
- chemical , therapeutic use : Peptides.
- Adoptive Transfer, Amino Acid Sequence, Animals, Cell Line, Drug Design, Mice, Molecular Sequence Data.
Abstract
Myelin basic protein (MBP) is a major candidate autoantigen in multiple sclerosis (MS). Its immunodominant epitope, MBP 85-99, forms a complex with human leukocyte antigen (HLA)-DR2 with which multiple sclerosis is genetically associated. Copolymer 1 (Copaxone), a random amino acid copolymer [poly (Y,E,A,K)n] as well as two modified synthetic copolymers [poly (F,Y,A,K)n and poly (V,W,A,K)n] also form complexes with HLA-DR2 (DRA/DRB1*1501) and compete with MBP 85-99 for binding. Moreover, two high-affinity synthetic peptide 15-mers that could inhibit binding even more effectively were previously designed. Here, we show that further-modified peptide 15-mers inhibited even more strongly (in order J5 > J3 > J2) both the binding of MBP 85-99 to HLA-DR2 and IL-2 production by two MBP 85-99-specific HLA-DR2-restricted T cells. J5, J3, and J2 also suppressed both MBP 85-99-induced experimental autoimmune encephalomyelitis (EAE) in humanized mice and proteolipid protein 139-151-induced EAE in SJL/J mice. Moreover, none of these previously uncharacterized peptide inhibitors crossreacted with MBP 85-99- or proteolipid protein 139-151-specific T cells. In both cases, spleen and lymph node cultures stimulated with these peptides produced large amounts of Th2 cytokines (IL-4 and IL-10), and adoptive transfer of established T cell lines suppressed disease induction. These peptide 15-mers provide specific, nonrandom sequences that appear to be at least as effective as random copolymers in suppressing EAE in several models.
DOI: 10.1073/pnas.0409022102
PubMed: 15665083
Affiliations:
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Le document en format XML
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Keskin</name></author><author><name sortKey="Fridkis Hareli, Masha" sort="Fridkis Hareli, Masha" uniqKey="Fridkis Hareli M" first="Masha" last="Fridkis-Hareli">Masha Fridkis-Hareli</name></author><author><name sortKey="Kuchroo, Vijay K" sort="Kuchroo, Vijay K" uniqKey="Kuchroo V" first="Vijay K" last="Kuchroo">Vijay K. Kuchroo</name></author><author><name sortKey="Strominger, Jack L" sort="Strominger, Jack L" uniqKey="Strominger J" first="Jack L" last="Strominger">Jack L. 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Stern</name><affiliation wicri:level="4"><nlm:affiliation>Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138</wicri:regionArea><placeName><region type="state">Massachusetts</region><settlement type="city">Cambridge (Massachusetts)</settlement></placeName><orgName type="university">Université Harvard</orgName></affiliation></author><author><name sortKey="Illes, Zsolt" sort="Illes, Zsolt" uniqKey="Illes Z" first="Zsolt" last="Illés">Zsolt Illés</name></author><author><name sortKey="Reddy, Jayagopala" sort="Reddy, Jayagopala" uniqKey="Reddy J" first="Jayagopala" last="Reddy">Jayagopala Reddy</name></author><author><name sortKey="Keskin, Derin B" sort="Keskin, Derin B" uniqKey="Keskin D" first="Derin B" last="Keskin">Derin B. Keskin</name></author><author><name sortKey="Fridkis Hareli, Masha" sort="Fridkis Hareli, Masha" uniqKey="Fridkis Hareli M" first="Masha" last="Fridkis-Hareli">Masha Fridkis-Hareli</name></author><author><name sortKey="Kuchroo, Vijay K" sort="Kuchroo, Vijay K" uniqKey="Kuchroo V" first="Vijay K" last="Kuchroo">Vijay K. Kuchroo</name></author><author><name sortKey="Strominger, Jack L" sort="Strominger, Jack L" uniqKey="Strominger J" first="Jack L" last="Strominger">Jack L. Strominger</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adoptive Transfer</term><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Drug Design</term><term>Encephalomyelitis, Autoimmune, Experimental (drug therapy)</term><term>Encephalomyelitis, Autoimmune, Experimental (immunology)</term><term>Mice</term><term>Molecular Sequence Data</term><term>Peptides (chemical synthesis)</term><term>Peptides (chemistry)</term><term>Peptides (pharmacology)</term><term>Peptides (therapeutic use)</term><term>T-Lymphocytes (drug effects)</term><term>T-Lymphocytes (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Conception de médicament</term><term>Données de séquences moléculaires</term><term>Encéphalomyélite auto-immune expérimentale (immunologie)</term><term>Encéphalomyélite auto-immune expérimentale (traitement médicamenteux)</term><term>Lignée cellulaire</term><term>Lymphocytes T ()</term><term>Lymphocytes T (immunologie)</term><term>Peptides ()</term><term>Peptides (pharmacologie)</term><term>Peptides (synthèse chimique)</term><term>Peptides (usage thérapeutique)</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Transfert adoptif</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Encephalomyelitis, Autoimmune, Experimental</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Encéphalomyélite auto-immune expérimentale</term><term>Lymphocytes T</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Encephalomyelitis, Autoimmune, Experimental</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="therapeutic use" xml:lang="en"><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Encéphalomyélite auto-immune expérimentale</term></keywords><keywords scheme="MESH" qualifier="usage thérapeutique" xml:lang="fr"><term>Peptides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adoptive Transfer</term><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Drug Design</term><term>Mice</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Conception de médicament</term><term>Données de séquences moléculaires</term><term>Lignée cellulaire</term><term>Lymphocytes T</term><term>Peptides</term><term>Souris</term><term>Séquence d'acides aminés</term><term>Transfert adoptif</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Myelin basic protein (MBP) is a major candidate autoantigen in multiple sclerosis (MS). Its immunodominant epitope, MBP 85-99, forms a complex with human leukocyte antigen (HLA)-DR2 with which multiple sclerosis is genetically associated. Copolymer 1 (Copaxone), a random amino acid copolymer [poly (Y,E,A,K)n] as well as two modified synthetic copolymers [poly (F,Y,A,K)n and poly (V,W,A,K)n] also form complexes with HLA-DR2 (DRA/DRB1*1501) and compete with MBP 85-99 for binding. Moreover, two high-affinity synthetic peptide 15-mers that could inhibit binding even more effectively were previously designed. Here, we show that further-modified peptide 15-mers inhibited even more strongly (in order J5 > J3 > J2) both the binding of MBP 85-99 to HLA-DR2 and IL-2 production by two MBP 85-99-specific HLA-DR2-restricted T cells. J5, J3, and J2 also suppressed both MBP 85-99-induced experimental autoimmune encephalomyelitis (EAE) in humanized mice and proteolipid protein 139-151-induced EAE in SJL/J mice. Moreover, none of these previously uncharacterized peptide inhibitors crossreacted with MBP 85-99- or proteolipid protein 139-151-specific T cells. In both cases, spleen and lymph node cultures stimulated with these peptides produced large amounts of Th2 cytokines (IL-4 and IL-10), and adoptive transfer of established T cell lines suppressed disease induction. These peptide 15-mers provide specific, nonrandom sequences that appear to be at least as effective as random copolymers in suppressing EAE in several models.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Massachusetts</li></region><settlement><li>Cambridge (Massachusetts)</li></settlement><orgName><li>Université Harvard</li></orgName></list><tree><noCountry><name sortKey="Fridkis Hareli, Masha" sort="Fridkis Hareli, Masha" uniqKey="Fridkis Hareli M" first="Masha" last="Fridkis-Hareli">Masha Fridkis-Hareli</name><name sortKey="Illes, Zsolt" sort="Illes, Zsolt" uniqKey="Illes Z" first="Zsolt" last="Illés">Zsolt Illés</name><name sortKey="Keskin, Derin B" sort="Keskin, Derin B" uniqKey="Keskin D" first="Derin B" last="Keskin">Derin B. Keskin</name><name sortKey="Kuchroo, Vijay K" sort="Kuchroo, Vijay K" uniqKey="Kuchroo V" first="Vijay K" last="Kuchroo">Vijay K. Kuchroo</name><name sortKey="Reddy, Jayagopala" sort="Reddy, Jayagopala" uniqKey="Reddy J" first="Jayagopala" last="Reddy">Jayagopala Reddy</name><name sortKey="Strominger, Jack L" sort="Strominger, Jack L" uniqKey="Strominger J" first="Jack L" last="Strominger">Jack L. Strominger</name></noCountry><country name="États-Unis"><region name="Massachusetts"><name sortKey="Stern, Joel N H" sort="Stern, Joel N H" uniqKey="Stern J" first="Joel N H" last="Stern">Joel N H. Stern</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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