MersV1, Main, Exploration, bibRecordById, pubmed:12122023

Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.

Identifieur interne : 003326 ( Main/Exploration ); précédent : 003325; suivant : 003327

Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.

Auteurs : Akira Tawada [Japon] ; Takahiro Masa ; Yoji Oonuki ; Atsushi Watanabe ; Yuji Matsuzaki ; Akira Asari

Source :

Glycobiology [ 0959-6658 ] ; 2002.

RBID : pubmed:12122023

Descripteurs français

KwdFr :
- Acide hyaluronique (), Acide hyaluronique (isolement et purification), Acide hyaluronique (synthèse chimique), Chromatographie en phase liquide à haute performance, Oligosaccharides (), Oligosaccharides (isolement et purification), Oligosaccharides (synthèse chimique), Spectrométrie de masse ESI, Électrophorèse ().
MESH :
- isolement et purification : Acide hyaluronique, Oligosaccharides.
- synthèse chimique : Acide hyaluronique, Oligosaccharides.
- Acide hyaluronique, Chromatographie en phase liquide à haute performance, Oligosaccharides, Spectrométrie de masse ESI, Électrophorèse.

English descriptors

KwdEn :
- Chromatography, High Pressure Liquid, Electrophoresis (methods), Hyaluronic Acid (chemical synthesis), Hyaluronic Acid (chemistry), Hyaluronic Acid (isolation & purification), Oligosaccharides (chemical synthesis), Oligosaccharides (chemistry), Oligosaccharides (isolation & purification), Spectrometry, Mass, Electrospray Ionization.
MESH :
- chemical , chemical synthesis : Hyaluronic Acid, Oligosaccharides.
- chemical , chemistry : Hyaluronic Acid, Oligosaccharides.
- chemical , isolation & purification : Hyaluronic Acid, Oligosaccharides.
- methods : Electrophoresis.
- Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization.

Abstract

Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.

DOI: 10.1093/glycob/cwf048
PubMed: 12122023

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en">Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.</div>
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Serveur d'exploration MERS

Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.

Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki

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