Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus
Identifieur interne : 000A05 ( Main/Exploration ); précédent : 000A04; suivant : 000A06Development of fluorescent reverse transcription loop-mediated isothermal amplification (RT-LAMP) using quenching probes for the detection of the Middle East respiratory syndrome coronavirus
Auteurs : Kazuya Shirato [Japon] ; Shohei Semba [Japon] ; Sherif A. El-Kafrawy [Arabie saoudite] ; Ahmed M. Hassan [Arabie saoudite] ; Ahmed M. Tolah [Arabie saoudite] ; Ikuyo Takayama [Japon] ; Tsutomu Kageyama [Japon] ; Tsugunori Notomi [Japon] ; Wataru Kamitani [Japon] ; Shutoku Matsuyama [Japon] ; Esam Ibraheem Azhar [Arabie saoudite]Source :
- Journal of Virological Methods [ 0166-0934 ] ; 2018.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Animaux, Arabie saoudite, Chameaux, Coronavirus du syndrome respiratoire du Moyen-Orient (génétique), Coronavirus du syndrome respiratoire du Moyen-Orient (isolement et purification), Fluorescence, Humains, Infections à coronavirus (diagnostic), Infections à coronavirus (médecine vétérinaire), Sensibilité et spécificité, Sondes oligonucléotidiques (génétique), Techniques d'amplification d'acides nucléiques (), Techniques de diagnostic moléculaire ().
- MESH :
- diagnostic : Infections à coronavirus.
- génétique : Amorces ADN, Coronavirus du syndrome respiratoire du Moyen-Orient, Sondes oligonucléotidiques.
- isolement et purification : Coronavirus du syndrome respiratoire du Moyen-Orient.
- médecine vétérinaire : Infections à coronavirus.
- Animaux, Arabie saoudite, Chameaux, Fluorescence, Humains, Sensibilité et spécificité, Techniques d'amplification d'acides nucléiques, Techniques de diagnostic moléculaire.
- Wicri :
- geographic : Arabie saoudite.
English descriptors
- KwdEn :
- Animals, Camelus, Coronavirus Infections (diagnosis), Coronavirus Infections (veterinary), DNA Primers (genetics), Fluorescence, Humans, Middle East Respiratory Syndrome Coronavirus (genetics), Middle East Respiratory Syndrome Coronavirus (isolation & purification), Molecular Diagnostic Techniques (methods), Nucleic Acid Amplification Techniques (methods), Oligonucleotide Probes (genetics), Saudi Arabia, Sensitivity and Specificity.
- MESH :
- chemical , genetics : DNA Primers, Oligonucleotide Probes.
- geographic : Saudi Arabia.
- diagnosis : Coronavirus Infections.
- genetics : Middle East Respiratory Syndrome Coronavirus.
- isolation & purification : Middle East Respiratory Syndrome Coronavirus.
- methods : Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques.
- veterinary : Coronavirus Infections.
- Animals, Camelus, Fluorescence, Humans, Sensitivity and Specificity.
Abstract
Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed. Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism. Only primer-derived signal can be monitored specifically by QProbes. Two primer sets were developed to enable to confirm MERS case by RT-LAMP only. Both sets were highly specific and sensitive in comparison with real-time RT-PCR.
Url:
DOI: 10.1016/j.jviromet.2018.05.006
PubMed: 29763640
PubMed Central: 7113683
Affiliations:
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Le document en format XML
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term>
<term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
<term>Sondes oligonucléotidiques</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Middle East Respiratory Syndrome Coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Coronavirus du syndrome respiratoire du Moyen-Orient</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term>
<term>Nucleic Acid Amplification Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Camelus</term>
<term>Fluorescence</term>
<term>Humans</term>
<term>Sensitivity and Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Arabie saoudite</term>
<term>Chameaux</term>
<term>Fluorescence</term>
<term>Humains</term>
<term>Sensibilité et spécificité</term>
<term>Techniques d'amplification d'acides nucléiques</term>
<term>Techniques de diagnostic moléculaire</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Arabie saoudite</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Highlights</title>
<p><list list-type="simple" id="lis0005"><list-item id="lsti0005"><label>•</label>
<p id="par0005">Fluorescent RT-LAMP assays using quenching probes for MERS-CoV were developed.</p>
</list-item>
<list-item id="lsti0010"><label>•</label>
<p id="par0010">Quenching probe (QProbe) can solve the problem in turbidity monitoring mechanism.</p>
</list-item>
<list-item id="lsti0015"><label>•</label>
<p id="par0015">Only primer-derived signal can be monitored specifically by QProbes.</p>
</list-item>
<list-item id="lsti0020"><label>•</label>
<p id="par0020">Two primer sets were developed to enable to confirm MERS case by RT-LAMP only.</p>
</list-item>
<list-item id="lsti0025"><label>•</label>
<p id="par0025">Both sets were highly specific and sensitive in comparison with real-time RT-PCR.</p>
</list-item>
</list>
</p>
</div>
</front>
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<author><name sortKey="Matsuyama, S" uniqKey="Matsuyama S">S. Matsuyama</name>
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<author><name sortKey="Yanagi, Y" uniqKey="Yanagi Y">Y. Yanagi</name>
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</listBibl>
</div1>
</back>
</TEI>
<affiliations><list><country><li>Arabie saoudite</li>
<li>Japon</li>
</country>
<region><li>Région de Kantō</li>
</region>
<settlement><li>Tokyo</li>
</settlement>
</list>
<tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Shirato, Kazuya" sort="Shirato, Kazuya" uniqKey="Shirato K" first="Kazuya" last="Shirato">Kazuya Shirato</name>
</region>
<name sortKey="Kageyama, Tsutomu" sort="Kageyama, Tsutomu" uniqKey="Kageyama T" first="Tsutomu" last="Kageyama">Tsutomu Kageyama</name>
<name sortKey="Kamitani, Wataru" sort="Kamitani, Wataru" uniqKey="Kamitani W" first="Wataru" last="Kamitani">Wataru Kamitani</name>
<name sortKey="Matsuyama, Shutoku" sort="Matsuyama, Shutoku" uniqKey="Matsuyama S" first="Shutoku" last="Matsuyama">Shutoku Matsuyama</name>
<name sortKey="Notomi, Tsugunori" sort="Notomi, Tsugunori" uniqKey="Notomi T" first="Tsugunori" last="Notomi">Tsugunori Notomi</name>
<name sortKey="Semba, Shohei" sort="Semba, Shohei" uniqKey="Semba S" first="Shohei" last="Semba">Shohei Semba</name>
<name sortKey="Takayama, Ikuyo" sort="Takayama, Ikuyo" uniqKey="Takayama I" first="Ikuyo" last="Takayama">Ikuyo Takayama</name>
</country>
<country name="Arabie saoudite"><noRegion><name sortKey="El Kafrawy, Sherif A" sort="El Kafrawy, Sherif A" uniqKey="El Kafrawy S" first="Sherif A." last="El-Kafrawy">Sherif A. El-Kafrawy</name>
</noRegion>
<name sortKey="Azhar, Esam Ibraheem" sort="Azhar, Esam Ibraheem" uniqKey="Azhar E" first="Esam Ibraheem" last="Azhar">Esam Ibraheem Azhar</name>
<name sortKey="Azhar, Esam Ibraheem" sort="Azhar, Esam Ibraheem" uniqKey="Azhar E" first="Esam Ibraheem" last="Azhar">Esam Ibraheem Azhar</name>
<name sortKey="Hassan, Ahmed M" sort="Hassan, Ahmed M" uniqKey="Hassan A" first="Ahmed M." last="Hassan">Ahmed M. Hassan</name>
<name sortKey="Tolah, Ahmed M" sort="Tolah, Ahmed M" uniqKey="Tolah A" first="Ahmed M." last="Tolah">Ahmed M. Tolah</name>
</country>
</tree>
</affiliations>
</record>
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