Spectrophotometric assay with turbidity correction of sized immunoglobulin G aggregates
Identifieur interne : 004F98 ( Main/Exploration ); précédent : 004F97; suivant : 004F99Spectrophotometric assay with turbidity correction of sized immunoglobulin G aggregates
Auteurs : Irwin Oreskes [États-Unis] ; David Mandel [États-Unis]Source :
- Analytical Biochemistry [ 0003-2697 ] ; 1979.
English descriptors
- KwdEn :
- Teeft :
- Absorbance, Absorbance measurements, Absorbance readings, Agarose columns, Aggregate, Aggregated, Apparent absorbance, Assay, Biological fluids, Brugge symposium, Correction procedure, Human immunoglobulin, Hypothetical turbidity values, Immune complexes, Immunoglobulin, Molecular weight, Particle size, Pathological effects, Permeation chromatography, Pigment suspensions, Rayleigh rule, Relative turbidity, Rheumatoid factor, Significant differences, Similar phenomena, Solid line, Spectrophotometric assay, Spherical particles, Total protein, True absorbance, Turbidity, Turbidity correction method, Turbidity effects, Turbidity measurements, Unit weight.
Abstract
Human immunoglobulin G (IgG) has been thermally aggregated and fractionated on calibrated agarose columns into cuts of known molecular weight. Quantitation of aggregates was done by spectrophotometry employing apparent absorbance measurements at two wavelengths. An equation has been developed to correct such data for turbidity or light-scattering effects. The validity of the method was demonstrated by comparison with the biuret and Kjeldahl techniques for total protein. It has been shown that the relative turbidity per unit weight of IgG aggregates increased regularly with particle size at least up to a molecular weight of 8 × 106. When relative turbidity per particle was determined (at a wavelength of 280 nm) a maximum value was obtained with particles of 8 × 105Mr (5-mers). Larger particles exhibited decreased turbidity. Similar phenomena have been reported for pigment suspensions, mineral colloids, and fog droplets. It is suggested that the pathological effects of immune complexes may be related to their size and that turbidity measurements may be useful in assessing this parameter.
Url:
DOI: 10.1016/S0003-2697(79)80018-4
Affiliations:
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Quantitation of aggregates was done by spectrophotometry employing apparent absorbance measurements at two wavelengths. An equation has been developed to correct such data for turbidity or light-scattering effects. The validity of the method was demonstrated by comparison with the biuret and Kjeldahl techniques for total protein. It has been shown that the relative turbidity per unit weight of IgG aggregates increased regularly with particle size at least up to a molecular weight of 8 × 106. When relative turbidity per particle was determined (at a wavelength of 280 nm) a maximum value was obtained with particles of 8 × 105Mr (5-mers). Larger particles exhibited decreased turbidity. Similar phenomena have been reported for pigment suspensions, mineral colloids, and fog droplets. It is suggested that the pathological effects of immune complexes may be related to their size and that turbidity measurements may be useful in assessing this parameter.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Oreskes, Irwin" sort="Oreskes, Irwin" uniqKey="Oreskes I" first="Irwin" last="Oreskes">Irwin Oreskes</name></noRegion><name sortKey="Mandel, David" sort="Mandel, David" uniqKey="Mandel D" first="David" last="Mandel">David Mandel</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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