Presence of globin gene transcripts in chicken oocytes and of a partially processed globin RNA in early embryos
Identifieur interne : 004B19 ( Main/Exploration ); précédent : 004B18; suivant : 004B20Presence of globin gene transcripts in chicken oocytes and of a partially processed globin RNA in early embryos
Auteurs : Tereza Imaizumi-Scherrer [France] ; Zehava Rangini [France, Israël] ; Christine Vesque [France] ; Philippe Duqesnoy [France] ; Klaus Scherrer [France]Source :
- Differentiation [ 0301-4681 ] ; 1989.
English descriptors
- Teeft :
- Additional sequences, Adult chicken erythroblasts, Amplification, Amplification reaction, Avian, Biol, Blastula, Cdna, Chicken embryos, Chicken oocytes, Cytoplasmic, Different stages, Digestion, Early embryos, Embryo, Embryonic, Embryonic erythroblasts, Embryonic transcripts, Erythroblast, Erythrocyte, Erythroid, Erythroid cells, Exon, Gastrula, Gene, Genomic, Globin, Globin cdna probes, Globin gene, Globin gene transcripts, Globin genes, Globin mrna, Globin mrnas, Globin probe, Globin probes, Globin synthesis, Globin transcript, Hybridization, Hybridized, Hybridizing, Kochav, Lymphoma, Mrna, Nucleotide, Oligomer, Oligomers, Oligonucleotides, Oocyte, Polymerase, Polymerase chain reaction, Primer, Probe, Protein synthesis, Right panel, Rna, Rnase, Scherrer, Stringent washes, Total cytoplasmic, Transcript.
Abstract
Abstract RNA isolated from chicken oocytes and early embryos of various stages of development were probed with cloned cDNA of the type (, D and A) and type (A, globin genes. Transcripts of all genes were present, although at a very low level, in the RNA of oocytes, and of embryos of the blastula and gastrula stages, prior to the onset of globin synthesis at about 30h incubation. Interestingly, Northern blotting of electrophoretically fractionated embryonic RNA made it possible to observe, at all stages of development and for all genes tested, RNA molecules several hundred nucleotides longer than mature mRNA. PCR amplification of the globin transcripts indicates that these additional sequences are localized upstream of the CAP site. These higher-MW forms were found to be replaced by normal-size globin mRNA several hours after the onset of globin synthesis. The relevance of these data to comprehension of how globin gene expression is controlled during development is discussed.
Url:
DOI: 10.1111/j.1432-0436.1989.tb00728.x
Affiliations:
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nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Life Sciences, Department of Zoology, Jerusalem</wicri:regionArea><wicri:noRegion>Jerusalem</wicri:noRegion></affiliation></author><author><name sortKey="Vesque, Christine" sort="Vesque, Christine" uniqKey="Vesque C" first="Christine" last="Vesque">Christine Vesque</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut Jacques Monod, Universit Paris 7, 2 Place Jussieu, F-75251 Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Gntique Molculaire, Ecole Normale Suprieure, 46, rue d'Ulm, F-75005 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Duqesnoy, Philippe" sort="Duqesnoy, Philippe" uniqKey="Duqesnoy P" first="Philippe" last="Duqesnoy">Philippe Duqesnoy</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Laboratoire de Gntique Molculaire, Centre Hospitalier et Universitaire Henri Mondor, Ave du Gnral de Gaulle, F-94010 Creteil</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Créteil</settlement></placeName></affiliation></author><author><name sortKey="Scherrer, Klaus" sort="Scherrer, Klaus" uniqKey="Scherrer K" first="Klaus" last="Scherrer">Klaus Scherrer</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>Institut Jacques Monod, Universit Paris 7, 2 Place Jussieu, F-75251 Paris Cedex 05</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Differentiation</title><title level="j" type="abbrev">DIFF</title><idno type="ISSN">0301-4681</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">41</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="22">22</biblScope><biblScope unit="page" to="33">33</biblScope></imprint><idno type="ISSN">0301-4681</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0301-4681</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Additional sequences</term><term>Adult chicken erythroblasts</term><term>Amplification</term><term>Amplification reaction</term><term>Avian</term><term>Biol</term><term>Blastula</term><term>Cdna</term><term>Chicken embryos</term><term>Chicken oocytes</term><term>Cytoplasmic</term><term>Different stages</term><term>Digestion</term><term>Early embryos</term><term>Embryo</term><term>Embryonic</term><term>Embryonic erythroblasts</term><term>Embryonic transcripts</term><term>Erythroblast</term><term>Erythrocyte</term><term>Erythroid</term><term>Erythroid cells</term><term>Exon</term><term>Gastrula</term><term>Gene</term><term>Genomic</term><term>Globin</term><term>Globin cdna probes</term><term>Globin gene</term><term>Globin gene transcripts</term><term>Globin genes</term><term>Globin mrna</term><term>Globin mrnas</term><term>Globin probe</term><term>Globin probes</term><term>Globin synthesis</term><term>Globin transcript</term><term>Hybridization</term><term>Hybridized</term><term>Hybridizing</term><term>Kochav</term><term>Lymphoma</term><term>Mrna</term><term>Nucleotide</term><term>Oligomer</term><term>Oligomers</term><term>Oligonucleotides</term><term>Oocyte</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Primer</term><term>Probe</term><term>Protein synthesis</term><term>Right panel</term><term>Rna</term><term>Rnase</term><term>Scherrer</term><term>Stringent washes</term><term>Total cytoplasmic</term><term>Transcript</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Abstract RNA isolated from chicken oocytes and early embryos of various stages of development were probed with cloned cDNA of the type (, D and A) and type (A, globin genes. Transcripts of all genes were present, although at a very low level, in the RNA of oocytes, and of embryos of the blastula and gastrula stages, prior to the onset of globin synthesis at about 30h incubation. Interestingly, Northern blotting of electrophoretically fractionated embryonic RNA made it possible to observe, at all stages of development and for all genes tested, RNA molecules several hundred nucleotides longer than mature mRNA. PCR amplification of the globin transcripts indicates that these additional sequences are localized upstream of the CAP site. These higher-MW forms were found to be replaced by normal-size globin mRNA several hours after the onset of globin synthesis. The relevance of these data to comprehension of how globin gene expression is controlled during development is discussed.</div></front></TEI><affiliations><list><country><li>France</li><li>Israël</li></country><region><li>Île-de-France</li></region><settlement><li>Créteil</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Imaizumi Scherrer, Tereza" sort="Imaizumi Scherrer, Tereza" uniqKey="Imaizumi Scherrer T" first="Tereza" last="Imaizumi-Scherrer">Tereza Imaizumi-Scherrer</name></region><name sortKey="Duqesnoy, Philippe" sort="Duqesnoy, Philippe" uniqKey="Duqesnoy P" first="Philippe" last="Duqesnoy">Philippe Duqesnoy</name><name sortKey="Rangini, Zehava" sort="Rangini, Zehava" uniqKey="Rangini Z" first="Zehava" last="Rangini">Zehava Rangini</name><name sortKey="Scherrer, Klaus" sort="Scherrer, Klaus" uniqKey="Scherrer K" first="Klaus" last="Scherrer">Klaus Scherrer</name><name sortKey="Vesque, Christine" sort="Vesque, Christine" uniqKey="Vesque C" first="Christine" last="Vesque">Christine Vesque</name><name sortKey="Vesque, Christine" sort="Vesque, Christine" uniqKey="Vesque C" first="Christine" last="Vesque">Christine Vesque</name></country><country name="Israël"><noRegion><name sortKey="Rangini, Zehava" sort="Rangini, Zehava" uniqKey="Rangini Z" first="Zehava" last="Rangini">Zehava Rangini</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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