Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize with Klebsiella pneumoniae
Identifieur interne : 004B13 ( Main/Exploration ); précédent : 004B12; suivant : 004B14Synthetic oligonucleotide probes complementary to the HLA-B variable region hybridize with Klebsiella pneumoniae
Auteurs : Kristina M. Williams [États-Unis] ; Takao Hirofuji [États-Unis] ; Richard B. Raybourne [États-Unis] ; Darlene L. Shook [États-Unis] ; Mary W. Trucksess [États-Unis] ; David T. Y. Yu [États-Unis]Source :
- Microbial Pathogenesis [ 0882-4010 ] ; 1989.
English descriptors
- Teeft :
- Amino, Amino acid, Amino acid residues, Amino acids, Ammonium acetate, Ankylosing, Ankylosing spondylitis, Appl environ, Bacterial species, Bacterial strains, Base composition, Base sequence, Biosystems model, Cdna, Celiac disease, Colony hybridization, Drug administration, Ethidium bromide, Fragment, Genes encoding, Genomic library, High stringency, Homology, Hybrid duplex, Hybridization, Hybridization mixture, Hybridize, Hybridized, Klebsiella, Klebsiella pneumoniae, Microbial pathogenesis, Mimicry, Molecular biology, Molecular mimicry, Monoclonal antibodies, Natural probe, Negative controls, Nucleic acids, Oligonucleotide, Oligonucleotide probes, Oligonucleotides, Optimum stringency, Oxytoca, Plasmid, Pneumoniae, Pneumoniae serotype, Positive controls, Possible role, Probe, Pstl, Pstl restriction fragment, Rabbit antisera, Recombinant, Recombinant plasmid, Rheumatoid arthritis, Sequence homology, Signal intensity, Similar sequences, Southern blot analysis, Specific binding, Specific hybridization, Spondylitis, Stringency, Synthetic oligodeoxyribonucleotides, Synthetic oligonucleotide probe, Synthetic oligonucleotide probes, Synthetic oligonucleotides, Synthetic probes, Transformants, Variable region, Yersinia enterocolitica.
Abstract
Abstract: Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66–74 of the HLA-B27.1 sequence and one corresponding to residues 66–74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb Pstl restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.
Url:
DOI: 10.1016/0882-4010(89)90101-0
Affiliations:
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Trucksess</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Contaminants Chemistry, Food and Drug Administration, Washington, DC 20204</wicri:regionArea><wicri:noRegion>DC 20204</wicri:noRegion></affiliation></author><author><name sortKey="Yu, David T Y" sort="Yu, David T Y" uniqKey="Yu D" first="David T. Y." last="Yu">David T. Y. Yu</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Rheumatology Division, Department of Medicine, University of California Los Angeles, Los Angeles, CA 90024</wicri:regionArea><wicri:noRegion>CA 90024</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Microbial Pathogenesis</title><title level="j" type="abbrev">YMPAT</title><idno type="ISSN">0882-4010</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1989">1989</date><biblScope unit="volume">6</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="277">277</biblScope><biblScope unit="page" to="286">286</biblScope></imprint><idno type="ISSN">0882-4010</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0882-4010</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amino</term><term>Amino acid</term><term>Amino acid residues</term><term>Amino acids</term><term>Ammonium acetate</term><term>Ankylosing</term><term>Ankylosing spondylitis</term><term>Appl environ</term><term>Bacterial species</term><term>Bacterial strains</term><term>Base composition</term><term>Base sequence</term><term>Biosystems model</term><term>Cdna</term><term>Celiac disease</term><term>Colony hybridization</term><term>Drug administration</term><term>Ethidium bromide</term><term>Fragment</term><term>Genes encoding</term><term>Genomic library</term><term>High stringency</term><term>Homology</term><term>Hybrid duplex</term><term>Hybridization</term><term>Hybridization mixture</term><term>Hybridize</term><term>Hybridized</term><term>Klebsiella</term><term>Klebsiella pneumoniae</term><term>Microbial pathogenesis</term><term>Mimicry</term><term>Molecular biology</term><term>Molecular mimicry</term><term>Monoclonal antibodies</term><term>Natural probe</term><term>Negative controls</term><term>Nucleic acids</term><term>Oligonucleotide</term><term>Oligonucleotide probes</term><term>Oligonucleotides</term><term>Optimum stringency</term><term>Oxytoca</term><term>Plasmid</term><term>Pneumoniae</term><term>Pneumoniae serotype</term><term>Positive controls</term><term>Possible role</term><term>Probe</term><term>Pstl</term><term>Pstl restriction fragment</term><term>Rabbit antisera</term><term>Recombinant</term><term>Recombinant plasmid</term><term>Rheumatoid arthritis</term><term>Sequence homology</term><term>Signal intensity</term><term>Similar sequences</term><term>Southern blot analysis</term><term>Specific binding</term><term>Specific hybridization</term><term>Spondylitis</term><term>Stringency</term><term>Synthetic oligodeoxyribonucleotides</term><term>Synthetic oligonucleotide probe</term><term>Synthetic oligonucleotide probes</term><term>Synthetic oligonucleotides</term><term>Synthetic probes</term><term>Transformants</term><term>Variable region</term><term>Yersinia enterocolitica</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Three oligonucleotide probes complementary to base sequences of the HLA-B variable region were used to probe clinical and foodborne isolates of Klebsiella spp. by DNA colony hybridization. One oligonucleotide (RR-3) corresponding to amino acid residues 66–74 of the HLA-B27.1 sequence and one corresponding to residues 66–74 of the HLA-B7 sequence (RR-5) hybridized with K. pneumoniae under conditions of high stringency. A genomic library was constructed using K. pneumoniae K43 chromosomal DNA, and a 1 kb Pstl restriction fragment was found to contain the sequence associated with specific binding of the oligonucleotide probes. After purification and radiolabeling, the cloned fragment hybridized with 96.2% of the K. pneumoniae isolates by DNA colony hybridization. These results confirm the presence of sequence similarities between bacterial DNA and the MHC Class I variable region.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Williams, Kristina M" sort="Williams, Kristina M" uniqKey="Williams K" first="Kristina M." last="Williams">Kristina M. Williams</name></noRegion><name sortKey="Hirofuji, Takao" sort="Hirofuji, Takao" uniqKey="Hirofuji T" first="Takao" last="Hirofuji">Takao Hirofuji</name><name sortKey="Raybourne, Richard B" sort="Raybourne, Richard B" uniqKey="Raybourne R" first="Richard B." last="Raybourne">Richard B. Raybourne</name><name sortKey="Shook, Darlene L" sort="Shook, Darlene L" uniqKey="Shook D" first="Darlene L." last="Shook">Darlene L. Shook</name><name sortKey="Trucksess, Mary W" sort="Trucksess, Mary W" uniqKey="Trucksess M" first="Mary W." last="Trucksess">Mary W. Trucksess</name><name sortKey="Yu, David T Y" sort="Yu, David T Y" uniqKey="Yu D" first="David T. Y." last="Yu">David T. Y. Yu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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