Amplification of a long sequence that includes a processed pseudogene for elongation factor 2 in the mouse.
Identifieur interne : 004A04 ( Main/Exploration ); précédent : 004A03; suivant : 004A05Amplification of a long sequence that includes a processed pseudogene for elongation factor 2 in the mouse.
Auteurs : T. Koide [Japon] ; M. Ishiura ; N. Hazumi ; T. Shiroishi ; Y. Okada ; T. UchidaSource :
- Genomics [ 0888-7543 ] ; 1990.
Descripteurs français
- KwdFr :
- Amplification de gène, Animaux, Cartographie de restriction, Cosmides (génétique), Données de séquences moléculaires, Facteur-2 d'élongation de la chaîne peptidique, Facteurs élongation chaîne peptidique (génétique), Pseudogènes, Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Électrophorèse.
- MESH :
- génétique : Cosmides, Facteurs élongation chaîne peptidique.
- Amplification de gène, Animaux, Cartographie de restriction, Données de séquences moléculaires, Facteur-2 d'élongation de la chaîne peptidique, Pseudogènes, Similitude de séquences d'acides nucléiques, Souris, Séquence nucléotidique, Électrophorèse.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Peptide Elongation Factors.
- chemical : Peptide Elongation Factor 2.
- genetics : Cosmids.
- Animals, Base Sequence, Electrophoresis, Gene Amplification, Mice, Molecular Sequence Data, Pseudogenes, Restriction Mapping, Sequence Homology, Nucleic Acid.
Abstract
Quantitative Southern blotting analysis has demonstrated that mouse cells contain about 70 copies per haploid genome of a DNA sequence related to the gene for elongation factor 2. The restriction maps of seven cosmids that each carry one copy of the EF2-related sequence (MER) and nucleotide sequences of MERs were highly conserved among the cosmids. Data obtained by such analyses suggest that MERs were produced by the integration of one copy of MER derived from poly(A)+ mRNA for EF2 into a specific site in the mouse genome, with subsequent amplification of MER together with its large flanking sequences during the evolution of the mouse. Furthermore, it appears that the size of each repeating unit is more than 60 kb. Analysis by pulse-field gel electrophoresis suggested that multiple copies of a repeating unit of more than 400 kb (or two units) are clustered at a specific site (or each specific site) in the mouse genome.
DOI: 10.1016/0888-7543(90)90450-9
PubMed: 2303263
Affiliations:
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Le document en format XML
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Shiroishi</name></author><author><name sortKey="Okada, Y" sort="Okada, Y" uniqKey="Okada Y" first="Y" last="Okada">Y. Okada</name></author><author><name sortKey="Uchida, T" sort="Uchida, T" uniqKey="Uchida T" first="T" last="Uchida">T. 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Koide</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cell Biology, National Institute for Basic Biology, Aichi, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Cell Biology, National Institute for Basic Biology, Aichi</wicri:regionArea><wicri:noRegion>Aichi</wicri:noRegion></affiliation></author><author><name sortKey="Ishiura, M" sort="Ishiura, M" uniqKey="Ishiura M" first="M" last="Ishiura">M. Ishiura</name></author><author><name sortKey="Hazumi, N" sort="Hazumi, N" uniqKey="Hazumi N" first="N" last="Hazumi">N. Hazumi</name></author><author><name sortKey="Shiroishi, T" sort="Shiroishi, T" uniqKey="Shiroishi T" first="T" last="Shiroishi">T. Shiroishi</name></author><author><name sortKey="Okada, Y" sort="Okada, Y" uniqKey="Okada Y" first="Y" last="Okada">Y. Okada</name></author><author><name sortKey="Uchida, T" sort="Uchida, T" uniqKey="Uchida T" first="T" last="Uchida">T. Uchida</name></author></analytic><series><title level="j">Genomics</title><idno type="ISSN">0888-7543</idno><imprint><date when="1990" type="published">1990</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cosmids (genetics)</term><term>Electrophoresis</term><term>Gene Amplification</term><term>Mice</term><term>Molecular Sequence Data</term><term>Peptide Elongation Factor 2</term><term>Peptide Elongation Factors (genetics)</term><term>Pseudogenes</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amplification de gène</term><term>Animaux</term><term>Cartographie de restriction</term><term>Cosmides (génétique)</term><term>Données de séquences moléculaires</term><term>Facteur-2 d'élongation de la chaîne peptidique</term><term>Facteurs élongation chaîne peptidique (génétique)</term><term>Pseudogènes</term><term>Similitude de séquences d'acides nucléiques</term><term>Souris</term><term>Séquence nucléotidique</term><term>Électrophorèse</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Peptide Elongation Factors</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Peptide Elongation Factor 2</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cosmids</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Cosmides</term><term>Facteurs élongation chaîne peptidique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Electrophoresis</term><term>Gene Amplification</term><term>Mice</term><term>Molecular Sequence Data</term><term>Pseudogenes</term><term>Restriction Mapping</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amplification de gène</term><term>Animaux</term><term>Cartographie de restriction</term><term>Données de séquences moléculaires</term><term>Facteur-2 d'élongation de la chaîne peptidique</term><term>Pseudogènes</term><term>Similitude de séquences d'acides nucléiques</term><term>Souris</term><term>Séquence nucléotidique</term><term>Électrophorèse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Quantitative Southern blotting analysis has demonstrated that mouse cells contain about 70 copies per haploid genome of a DNA sequence related to the gene for elongation factor 2. The restriction maps of seven cosmids that each carry one copy of the EF2-related sequence (MER) and nucleotide sequences of MERs were highly conserved among the cosmids. Data obtained by such analyses suggest that MERs were produced by the integration of one copy of MER derived from poly(A)+ mRNA for EF2 into a specific site in the mouse genome, with subsequent amplification of MER together with its large flanking sequences during the evolution of the mouse. Furthermore, it appears that the size of each repeating unit is more than 60 kb. Analysis by pulse-field gel electrophoresis suggested that multiple copies of a repeating unit of more than 400 kb (or two units) are clustered at a specific site (or each specific site) in the mouse genome.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Hazumi, N" sort="Hazumi, N" uniqKey="Hazumi N" first="N" last="Hazumi">N. Hazumi</name><name sortKey="Ishiura, M" sort="Ishiura, M" uniqKey="Ishiura M" first="M" last="Ishiura">M. Ishiura</name><name sortKey="Okada, Y" sort="Okada, Y" uniqKey="Okada Y" first="Y" last="Okada">Y. Okada</name><name sortKey="Shiroishi, T" sort="Shiroishi, T" uniqKey="Shiroishi T" first="T" last="Shiroishi">T. Shiroishi</name><name sortKey="Uchida, T" sort="Uchida, T" uniqKey="Uchida T" first="T" last="Uchida">T. Uchida</name></noCountry><country name="Japon"><noRegion><name sortKey="Koide, T" sort="Koide, T" uniqKey="Koide T" first="T" last="Koide">T. Koide</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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