Theoretical and experimental measures of DNA helix stability and their relation to sequence specific repair of O6-ethylguanine lesions.
Identifieur interne : 004861 ( Main/Exploration ); précédent : 004860; suivant : 004862Theoretical and experimental measures of DNA helix stability and their relation to sequence specific repair of O6-ethylguanine lesions.
Auteurs : C K Foley [États-Unis] ; L G Pedersen ; T A Darden ; B W Glickman ; M W AndersonSource :
- Mutation research [ 0027-5107 ] ; 1991.
Descripteurs français
- KwdFr :
- ADN bactérien (), ADN bactérien (métabolisme), Altération de l'ADN, Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Escherichia coli (), Escherichia coli (génétique), Escherichia coli (métabolisme), Guanine (analogues et dérivés), Guanine (métabolisme), Méthanesulfonate d'éthyle (toxicité), Réparation de l'ADN (physiologie), Séquence nucléotidique, Thermodynamique.
- MESH :
- analogues et dérivés : Guanine.
- génétique : Escherichia coli.
- métabolisme : ADN bactérien, Escherichia coli, Guanine.
- physiologie : Réparation de l'ADN.
- toxicité : Méthanesulfonate d'éthyle.
- ADN bactérien, Altération de l'ADN, Cinétique, Conformation d'acide nucléique, Données de séquences moléculaires, Escherichia coli, Séquence nucléotidique, Thermodynamique.
English descriptors
- KwdEn :
- Base Sequence, DNA Damage, DNA Repair (physiology), DNA, Bacterial (chemistry), DNA, Bacterial (metabolism), Escherichia coli (drug effects), Escherichia coli (genetics), Escherichia coli (metabolism), Ethyl Methanesulfonate (toxicity), Guanine (analogs & derivatives), Guanine (metabolism), Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, Thermodynamics.
- MESH :
- chemical , analogs & derivatives : Guanine.
- chemical , chemistry : DNA, Bacterial.
- chemical , metabolism : DNA, Bacterial, Guanine.
- drug effects : Escherichia coli.
- genetics : Escherichia coli.
- metabolism : Escherichia coli.
- physiology : DNA Repair.
- chemical , toxicity : Ethyl Methanesulfonate.
- Base Sequence, DNA Damage, Kinetics, Molecular Sequence Data, Nucleic Acid Conformation, Thermodynamics.
Abstract
Recent work (Breslauer et al. (1986) Proc. Natl. Acad. Sci. (U.S.A.), 83, 3746) has provided a method for calculating empirical thermodynamic quantities for helix to coil transitions from the base sequence of any oligomer. It is shown in this work that the DNA helix binding energy, calculated with the AMBER force field, for 9-mers of the type 5'-GGGXGeYGGG-3', where X and Y are any base and the central Ge is O6-ethylguanine, correlates well with the empirical delta G for helix to strand transitions. The mutation spectrum of ethane methylsulfonate (EMS) in the lacI gene of Escherichia coli can be modeled using the calculated local binding energy but the empirical free energies, enthalpies and melting temperatures predict these levels of repair less well. The relation of the binding energy to the mutation spectrum can be somewhat improved by including entropic effects in a theoretical free energy of binding as given by delta G theoretical identical to delta E binding - T delta S.
DOI: 10.1016/0921-8777(91)90021-g
PubMed: 2067552
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002990
- to stream PubMed, to step Curation: 002990
- to stream PubMed, to step Checkpoint: 002821
- to stream Ncbi, to step Merge: 000767
- to stream Ncbi, to step Curation: 000767
- to stream Ncbi, to step Checkpoint: 000767
- to stream Main, to step Merge: 004935
- to stream Main, to step Curation: 004861
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Theoretical and experimental measures of DNA helix stability and their relation to sequence specific repair of O6-ethylguanine lesions.</title><author><name sortKey="Foley, C K" sort="Foley, C K" uniqKey="Foley C" first="C K" last="Foley">C K Foley</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park</wicri:cityArea></affiliation></author><author><name sortKey="Pedersen, L G" sort="Pedersen, L G" uniqKey="Pedersen L" first="L G" last="Pedersen">L G Pedersen</name></author><author><name sortKey="Darden, T A" sort="Darden, T A" uniqKey="Darden T" first="T A" last="Darden">T A Darden</name></author><author><name sortKey="Glickman, B W" sort="Glickman, B W" uniqKey="Glickman B" first="B W" last="Glickman">B W Glickman</name></author><author><name sortKey="Anderson, M W" sort="Anderson, M W" uniqKey="Anderson M" first="M W" last="Anderson">M W Anderson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1991">1991</date><idno type="RBID">pubmed:2067552</idno><idno type="pmid">2067552</idno><idno type="doi">10.1016/0921-8777(91)90021-g</idno><idno type="wicri:Area/PubMed/Corpus">002990</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002990</idno><idno type="wicri:Area/PubMed/Curation">002990</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002990</idno><idno type="wicri:Area/PubMed/Checkpoint">002821</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002821</idno><idno type="wicri:Area/Ncbi/Merge">000767</idno><idno type="wicri:Area/Ncbi/Curation">000767</idno><idno type="wicri:Area/Ncbi/Checkpoint">000767</idno><idno type="wicri:doubleKey">0027-5107:1991:Foley C:theoretical:and:experimental</idno><idno type="wicri:Area/Main/Merge">004935</idno><idno type="wicri:Area/Main/Curation">004861</idno><idno type="wicri:Area/Main/Exploration">004861</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Theoretical and experimental measures of DNA helix stability and their relation to sequence specific repair of O6-ethylguanine lesions.</title><author><name sortKey="Foley, C K" sort="Foley, C K" uniqKey="Foley C" first="C K" last="Foley">C K Foley</name><affiliation wicri:level="2"><nlm:affiliation>Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Caroline du Nord</region></placeName><wicri:cityArea>Laboratory of Molecular Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park</wicri:cityArea></affiliation></author><author><name sortKey="Pedersen, L G" sort="Pedersen, L G" uniqKey="Pedersen L" first="L G" last="Pedersen">L G Pedersen</name></author><author><name sortKey="Darden, T A" sort="Darden, T A" uniqKey="Darden T" first="T A" last="Darden">T A Darden</name></author><author><name sortKey="Glickman, B W" sort="Glickman, B W" uniqKey="Glickman B" first="B W" last="Glickman">B W Glickman</name></author><author><name sortKey="Anderson, M W" sort="Anderson, M W" uniqKey="Anderson M" first="M W" last="Anderson">M W Anderson</name></author></analytic><series><title level="j">Mutation research</title><idno type="ISSN">0027-5107</idno><imprint><date when="1991" type="published">1991</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>DNA Damage</term><term>DNA Repair (physiology)</term><term>DNA, Bacterial (chemistry)</term><term>DNA, Bacterial (metabolism)</term><term>Escherichia coli (drug effects)</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Ethyl Methanesulfonate (toxicity)</term><term>Guanine (analogs & derivatives)</term><term>Guanine (metabolism)</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Thermodynamics</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien ()</term><term>ADN bactérien (métabolisme)</term><term>Altération de l'ADN</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>Données de séquences moléculaires</term><term>Escherichia coli ()</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Guanine (analogues et dérivés)</term><term>Guanine (métabolisme)</term><term>Méthanesulfonate d'éthyle (toxicité)</term><term>Réparation de l'ADN (physiologie)</term><term>Séquence nucléotidique</term><term>Thermodynamique</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Guanine</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Bacterial</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Bacterial</term><term>Guanine</term></keywords><keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Guanine</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN bactérien</term><term>Escherichia coli</term><term>Guanine</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réparation de l'ADN</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>DNA Repair</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Ethyl Methanesulfonate</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Méthanesulfonate d'éthyle</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>DNA Damage</term><term>Kinetics</term><term>Molecular Sequence Data</term><term>Nucleic Acid Conformation</term><term>Thermodynamics</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN bactérien</term><term>Altération de l'ADN</term><term>Cinétique</term><term>Conformation d'acide nucléique</term><term>Données de séquences moléculaires</term><term>Escherichia coli</term><term>Séquence nucléotidique</term><term>Thermodynamique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recent work (Breslauer et al. (1986) Proc. Natl. Acad. Sci. (U.S.A.), 83, 3746) has provided a method for calculating empirical thermodynamic quantities for helix to coil transitions from the base sequence of any oligomer. It is shown in this work that the DNA helix binding energy, calculated with the AMBER force field, for 9-mers of the type 5'-GGGXGeYGGG-3', where X and Y are any base and the central Ge is O6-ethylguanine, correlates well with the empirical delta G for helix to strand transitions. The mutation spectrum of ethane methylsulfonate (EMS) in the lacI gene of Escherichia coli can be modeled using the calculated local binding energy but the empirical free energies, enthalpies and melting temperatures predict these levels of repair less well. The relation of the binding energy to the mutation spectrum can be somewhat improved by including entropic effects in a theoretical free energy of binding as given by delta G theoretical identical to delta E binding - T delta S.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><noCountry><name sortKey="Anderson, M W" sort="Anderson, M W" uniqKey="Anderson M" first="M W" last="Anderson">M W Anderson</name><name sortKey="Darden, T A" sort="Darden, T A" uniqKey="Darden T" first="T A" last="Darden">T A Darden</name><name sortKey="Glickman, B W" sort="Glickman, B W" uniqKey="Glickman B" first="B W" last="Glickman">B W Glickman</name><name sortKey="Pedersen, L G" sort="Pedersen, L G" uniqKey="Pedersen L" first="L G" last="Pedersen">L G Pedersen</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Foley, C K" sort="Foley, C K" uniqKey="Foley C" first="C K" last="Foley">C K Foley</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004861 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004861 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:2067552
|texte= Theoretical and experimental measures of DNA helix stability and their relation to sequence specific repair of O6-ethylguanine lesions.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:2067552" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a MersV1
| This area was generated with Dilib version V0.6.33. |  |