Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Penetration into Target Cells by Synthetic Peptides Mimicking the N-Terminus of the HIV-1 Transmembrane Glycoprotein
Identifieur interne : 004650 ( Main/Exploration ); précédent : 004649; suivant : 004651Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Penetration into Target Cells by Synthetic Peptides Mimicking the N-Terminus of the HIV-1 Transmembrane Glycoprotein
Auteurs : Vladimir A. Slepushkin [Russie] ; Galina V. Kornilaeva [Russie] ; Sergei M. Andreev [Russie] ; Maria V. Sidorova [Russie] ; Anna O. Petrukhina [Russie] ; Gennady R. Matsevich [Russie] ; Svetlana V. Raduk [Russie] ; Vladimir B. Grigoriev [Russie] ; Tatiana V. Makarova [Russie] ; Vladimir V. Lukashov [Russie] ; Edvard V. Karamov [Russie]Source :
- Virology [ 0042-6822 ] ; 1993.
Abstract
Abstract: To investigate the mechanism of action of the 22-amino-acid HIV fusion peptide on HIV infection, we studied its influence on virus adsorption and HIV-induced syncytium formation. The effect of the peptide preparations on the synthesis of viral antigens in HIV-infected cell cultures was determined by antigen capture assay, and the inhibition of proviral DNA synthesis was detected by hybridization with a HIV-specific oligonucleotide probe after PCR amplification. Fusion peptides inhibited HIV-induced syncytium formation and antigen production in lyric infected cells, and this effect was increased in conjugation with bovine serum albumin or with synthetic net-charged polymer by its C-terminus. The association of peptide with carrier by N-terminus, or with positive-charged polymer or gelatin completely abolished its effect on HIV infection. No peptide preparations influenced HIV-1 chronically infected cells. Because peptide preparations blocked the HIV-specific DNA synthesis 2 hr after infection without influencing virus adsorption and reverse transcription, we concluded that the block of infection occurred during the penetration of virions through the cell membrane. On the basis of results obtained we propose that our peptide preparations could be used for anti-HIV chemotherapy. The possibility of the existence of receptors for gp41 N-terminal region on target cell membrane is discussed.
Url:
DOI: 10.1006/viro.1993.1260
Affiliations:
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Slepushkin</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Kornilaeva, Galina V" sort="Kornilaeva, Galina V" uniqKey="Kornilaeva G" first="Galina V." last="Kornilaeva">Galina V. Kornilaeva</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Andreev, Sergei M" sort="Andreev, Sergei M" uniqKey="Andreev S" first="Sergei M." last="Andreev">Sergei M. Andreev</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Sidorova, Maria V" sort="Sidorova, Maria V" uniqKey="Sidorova M" first="Maria V." last="Sidorova">Maria V. Sidorova</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Petrukhina, Anna O" sort="Petrukhina, Anna O" uniqKey="Petrukhina A" first="Anna O." last="Petrukhina">Anna O. Petrukhina</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Matsevich, Gennady R" sort="Matsevich, Gennady R" uniqKey="Matsevich G" first="Gennady R." last="Matsevich">Gennady R. Matsevich</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Raduk, Svetlana V" sort="Raduk, Svetlana V" uniqKey="Raduk S" first="Svetlana V." last="Raduk">Svetlana V. Raduk</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Grigoriev, Vladimir B" sort="Grigoriev, Vladimir B" uniqKey="Grigoriev V" first="Vladimir B." last="Grigoriev">Vladimir B. Grigoriev</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Makarova, Tatiana V" sort="Makarova, Tatiana V" uniqKey="Makarova T" first="Tatiana V." last="Makarova">Tatiana V. Makarova</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Lukashov, Vladimir V" sort="Lukashov, Vladimir V" uniqKey="Lukashov V" first="Vladimir V." last="Lukashov">Vladimir V. Lukashov</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author><author><name sortKey="Karamov, Edvard V" sort="Karamov, Edvard V" uniqKey="Karamov E" first="Edvard V." last="Karamov">Edvard V. Karamov</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>D. I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, 16 Gamaleya St., 123098 Moscow, Russia; Institute of Immunology, Ministry of Public Health of Russia, 24/2 Kashirskoe Sh., 115478 Moscow, Russia; and Research Institute of Viral Preparations, Russian Academy of Medical Sciences, 15 1st Dubrovskaya St., 109088 Moscow</wicri:regionArea><placeName><settlement type="city">Moscou</settlement><region>District fédéral central</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">194</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="294">294</biblScope><biblScope unit="page" to="301">301</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: To investigate the mechanism of action of the 22-amino-acid HIV fusion peptide on HIV infection, we studied its influence on virus adsorption and HIV-induced syncytium formation. The effect of the peptide preparations on the synthesis of viral antigens in HIV-infected cell cultures was determined by antigen capture assay, and the inhibition of proviral DNA synthesis was detected by hybridization with a HIV-specific oligonucleotide probe after PCR amplification. Fusion peptides inhibited HIV-induced syncytium formation and antigen production in lyric infected cells, and this effect was increased in conjugation with bovine serum albumin or with synthetic net-charged polymer by its C-terminus. The association of peptide with carrier by N-terminus, or with positive-charged polymer or gelatin completely abolished its effect on HIV infection. No peptide preparations influenced HIV-1 chronically infected cells. Because peptide preparations blocked the HIV-specific DNA synthesis 2 hr after infection without influencing virus adsorption and reverse transcription, we concluded that the block of infection occurred during the penetration of virions through the cell membrane. On the basis of results obtained we propose that our peptide preparations could be used for anti-HIV chemotherapy. The possibility of the existence of receptors for gp41 N-terminal region on target cell membrane is discussed.</div></front></TEI><affiliations><list><country><li>Russie</li></country><region><li>District fédéral central</li></region><settlement><li>Moscou</li></settlement></list><tree><country name="Russie"><region name="District fédéral central"><name sortKey="Slepushkin, Vladimir A" sort="Slepushkin, Vladimir A" uniqKey="Slepushkin V" first="Vladimir A." last="Slepushkin">Vladimir A. Slepushkin</name></region><name sortKey="Andreev, Sergei M" sort="Andreev, Sergei M" uniqKey="Andreev S" first="Sergei M." last="Andreev">Sergei M. Andreev</name><name sortKey="Grigoriev, Vladimir B" sort="Grigoriev, Vladimir B" uniqKey="Grigoriev V" first="Vladimir B." last="Grigoriev">Vladimir B. Grigoriev</name><name sortKey="Karamov, Edvard V" sort="Karamov, Edvard V" uniqKey="Karamov E" first="Edvard V." last="Karamov">Edvard V. Karamov</name><name sortKey="Kornilaeva, Galina V" sort="Kornilaeva, Galina V" uniqKey="Kornilaeva G" first="Galina V." last="Kornilaeva">Galina V. Kornilaeva</name><name sortKey="Lukashov, Vladimir V" sort="Lukashov, Vladimir V" uniqKey="Lukashov V" first="Vladimir V." last="Lukashov">Vladimir V. Lukashov</name><name sortKey="Makarova, Tatiana V" sort="Makarova, Tatiana V" uniqKey="Makarova T" first="Tatiana V." last="Makarova">Tatiana V. Makarova</name><name sortKey="Matsevich, Gennady R" sort="Matsevich, Gennady R" uniqKey="Matsevich G" first="Gennady R." last="Matsevich">Gennady R. Matsevich</name><name sortKey="Petrukhina, Anna O" sort="Petrukhina, Anna O" uniqKey="Petrukhina A" first="Anna O." last="Petrukhina">Anna O. Petrukhina</name><name sortKey="Raduk, Svetlana V" sort="Raduk, Svetlana V" uniqKey="Raduk S" first="Svetlana V." last="Raduk">Svetlana V. Raduk</name><name sortKey="Sidorova, Maria V" sort="Sidorova, Maria V" uniqKey="Sidorova M" first="Maria V." last="Sidorova">Maria V. Sidorova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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