Induction of feline leukaemia virus-neutralizing antibodies by immunization with synthetic peptides derived from the FeLV env gene
Identifieur interne : 004571 ( Main/Exploration ); précédent : 004570; suivant : 004572Induction of feline leukaemia virus-neutralizing antibodies by immunization with synthetic peptides derived from the FeLV env gene
Auteurs : Kees Weijer [Pays-Bas] ; Anita Pfauth [Pays-Bas] ; Rob Van Herwijnen [Pays-Bas] ; Oswald Jarrett [Royaume-Uni] ; Rob H. Meloen [Pays-Bas] ; Chris Tomee [Pays-Bas] ; Albert D. M. E. Osterhaus [Pays-Bas]Source :
- Vaccine [ 0264-410X ] ; 1993.
English descriptors
- Teeft :
- Adjuvant, Amino acid sequence, Antibody, Antibody response, Antibody responses, Antigenic presentation, Booster, Different peptide elisas, Different peptides, Effective vaccine, Elisa, Empty iscoms, Envelope gene, Epitope, Feline, Feline leukaemia virus, Feline leukemia virus, Felv, Felv elisa, Felv immunization, Felv infection, First experiment, First immunization, Functional activity, Immunization, Immunoblotting, Iscom, Iscoms, Jarrett, Leukaemia, Mabs, Mgpnlv, Microwell plates, Monoclonal antibodies, Myristic acid, Myristylated peptides, Native protein, Natl acad, Negative control, Neutralizing, Neutralizing antibodies, Normal rabbit serum, Nucleotide sequence, Nucleotide sequences, Osterhaus, Other rabbits, Peptide, Peptide antibodies, Peptide combination, Peptide elisas, Peptides iscom, Polyclonal, Polyclonal antibodies, Rabbit, Same binding area, Same conjugates, Same peptides, Second experiment, Subgroup, Synthetic peptides, Tertiary structure, Tertiary structures, Unmyristylated peptides, Vaccine, Variable region, Weijer.
Abstract
Abstract: The surface glycoprotein, gp70, of feline leukaemia virus (FeLV) contains sites which are important in inducing neutralizing antibodies. Using synthetic peptides corresponding to nucleotide sequence 729–957 (amino acid sequence 243–319) of FeLV-A/Glasgow-1, the antigenicity and immunogenicity of this part of the viral surface glycoprotein were investigated. The region contains two highly conserved domains separated by a variable region (VR4), when compared with FeLV of subgroups B and C, and an epitope known to be involved in virus neutralization is located in the N-terminal conserved domain. Five murine monoclonal antibodies generated by immunization with virus were found to be directed to this domain and four were virus-neutralizing. Polyclonal mouse, rabbit and cat anti-FeLV antisera, which were virus-neutralizing, were directed to B-cell epitopes in the peptides. To determine if those synthetic peptides could induce neutralizing antibodies, rabbits were immunized with the peptides, singly or in combination. Antibody responses were measured by ELISA for anti-peptide, anti-FeLV and anti-gp70 activity, by immunoblotting, by membrane immunofluorescence and by virus-neutralization tests. Virus-neutralizing antibodies were induced by FeLV gp70 peptides and there was a synergistic effect on antibody production when a combination of peptides covering amino acid sequence 243–319 of FeLV-A was used. In a second experiment, six rabbits and six cats were immunized with a combination of two peptides, which covered the above-defined FeLV gp70 sequence. Comparisons were made of the responses to these peptides incorporated into immunostimulating complexes (ISCOMs) via myristic acid tails, inoculated with ‘empty’ ISCOMs, or with Al(OH)3. Complete Freund's adjuvant (CFA) had a very strong potentiating effect on the induction of antibodies and immunization with peptides incorporated into ISCOMs was superior to immunization using adjuvants other than CFA. It is very promising that not only in rabbits, but also in the natural host of FeLV, the cat, anti-FeLV gp70 (peptides) antibodies could be induced by FeLV gp70 peptides.
Url:
DOI: 10.1016/0264-410X(93)90384-A
Affiliations:
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European Veterinary Laboratory EVL, Amsterdam</wicri:regionArea><placeName><settlement type="city">Amsterdam</settlement><region nuts="2" type="province">Hollande-Septentrionale</region></placeName></affiliation></author><author><name sortKey="Jarrett, Oswald" sort="Jarrett, Oswald" uniqKey="Jarrett O" first="Oswald" last="Jarrett">Oswald Jarrett</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>University of Glasgow, Department of Veterinary Pathology, Glasgow</wicri:regionArea><orgName type="university">Université de Glasgow</orgName><placeName><settlement type="city">Glasgow</settlement><region type="country">Écosse</region></placeName></affiliation></author><author><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Central Veterinary Institute, Lelystad</wicri:regionArea><wicri:noRegion>Lelystad</wicri:noRegion></affiliation></author><author><name sortKey="Tomee, Chris" sort="Tomee, Chris" uniqKey="Tomee C" first="Chris" last="Tomee">Chris Tomee</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>The Netherlands Cancer Institute (Antoni van Leeuwenhoek Huis), Division of Immunology, Plesmanlaan 121, 1066 CX Amsterdam</wicri:regionArea><wicri:noRegion>1066 CX Amsterdam</wicri:noRegion></affiliation></author><author><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>The National Institute of Public Health and Environmental Hygiene, Bilthoven</wicri:regionArea><wicri:noRegion>Bilthoven</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Vaccine</title><title level="j" type="abbrev">JVAC</title><idno type="ISSN">0264-410X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">11</biblScope><biblScope unit="issue">9</biblScope><biblScope unit="page" from="946">946</biblScope><biblScope unit="page" to="956">956</biblScope></imprint><idno type="ISSN">0264-410X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0264-410X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Adjuvant</term><term>Amino acid sequence</term><term>Antibody</term><term>Antibody response</term><term>Antibody responses</term><term>Antigenic presentation</term><term>Booster</term><term>Different peptide elisas</term><term>Different peptides</term><term>Effective vaccine</term><term>Elisa</term><term>Empty iscoms</term><term>Envelope gene</term><term>Epitope</term><term>Feline</term><term>Feline leukaemia virus</term><term>Feline leukemia virus</term><term>Felv</term><term>Felv elisa</term><term>Felv immunization</term><term>Felv infection</term><term>First experiment</term><term>First immunization</term><term>Functional activity</term><term>Immunization</term><term>Immunoblotting</term><term>Iscom</term><term>Iscoms</term><term>Jarrett</term><term>Leukaemia</term><term>Mabs</term><term>Mgpnlv</term><term>Microwell plates</term><term>Monoclonal antibodies</term><term>Myristic acid</term><term>Myristylated peptides</term><term>Native protein</term><term>Natl acad</term><term>Negative control</term><term>Neutralizing</term><term>Neutralizing antibodies</term><term>Normal rabbit serum</term><term>Nucleotide sequence</term><term>Nucleotide sequences</term><term>Osterhaus</term><term>Other rabbits</term><term>Peptide</term><term>Peptide antibodies</term><term>Peptide combination</term><term>Peptide elisas</term><term>Peptides iscom</term><term>Polyclonal</term><term>Polyclonal antibodies</term><term>Rabbit</term><term>Same binding area</term><term>Same conjugates</term><term>Same peptides</term><term>Second experiment</term><term>Subgroup</term><term>Synthetic peptides</term><term>Tertiary structure</term><term>Tertiary structures</term><term>Unmyristylated peptides</term><term>Vaccine</term><term>Variable region</term><term>Weijer</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The surface glycoprotein, gp70, of feline leukaemia virus (FeLV) contains sites which are important in inducing neutralizing antibodies. Using synthetic peptides corresponding to nucleotide sequence 729–957 (amino acid sequence 243–319) of FeLV-A/Glasgow-1, the antigenicity and immunogenicity of this part of the viral surface glycoprotein were investigated. The region contains two highly conserved domains separated by a variable region (VR4), when compared with FeLV of subgroups B and C, and an epitope known to be involved in virus neutralization is located in the N-terminal conserved domain. Five murine monoclonal antibodies generated by immunization with virus were found to be directed to this domain and four were virus-neutralizing. Polyclonal mouse, rabbit and cat anti-FeLV antisera, which were virus-neutralizing, were directed to B-cell epitopes in the peptides. To determine if those synthetic peptides could induce neutralizing antibodies, rabbits were immunized with the peptides, singly or in combination. Antibody responses were measured by ELISA for anti-peptide, anti-FeLV and anti-gp70 activity, by immunoblotting, by membrane immunofluorescence and by virus-neutralization tests. Virus-neutralizing antibodies were induced by FeLV gp70 peptides and there was a synergistic effect on antibody production when a combination of peptides covering amino acid sequence 243–319 of FeLV-A was used. In a second experiment, six rabbits and six cats were immunized with a combination of two peptides, which covered the above-defined FeLV gp70 sequence. Comparisons were made of the responses to these peptides incorporated into immunostimulating complexes (ISCOMs) via myristic acid tails, inoculated with ‘empty’ ISCOMs, or with Al(OH)3. Complete Freund's adjuvant (CFA) had a very strong potentiating effect on the induction of antibodies and immunization with peptides incorporated into ISCOMs was superior to immunization using adjuvants other than CFA. It is very promising that not only in rabbits, but also in the natural host of FeLV, the cat, anti-FeLV gp70 (peptides) antibodies could be induced by FeLV gp70 peptides.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li><li>Royaume-Uni</li></country><region><li>Hollande-Septentrionale</li><li>Écosse</li></region><settlement><li>Amsterdam</li><li>Glasgow</li></settlement><orgName><li>Université de Glasgow</li></orgName></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Weijer, Kees" sort="Weijer, Kees" uniqKey="Weijer K" first="Kees" last="Weijer">Kees Weijer</name></noRegion><name sortKey="Meloen, Rob H" sort="Meloen, Rob H" uniqKey="Meloen R" first="Rob H." last="Meloen">Rob H. Meloen</name><name sortKey="Osterhaus, Albert D M E" sort="Osterhaus, Albert D M E" uniqKey="Osterhaus A" first="Albert D. M. E." last="Osterhaus">Albert D. M. E. Osterhaus</name><name sortKey="Pfauth, Anita" sort="Pfauth, Anita" uniqKey="Pfauth A" first="Anita" last="Pfauth">Anita Pfauth</name><name sortKey="Tomee, Chris" sort="Tomee, Chris" uniqKey="Tomee C" first="Chris" last="Tomee">Chris Tomee</name><name sortKey="Van Herwijnen, Rob" sort="Van Herwijnen, Rob" uniqKey="Van Herwijnen R" first="Rob" last="Van Herwijnen">Rob Van Herwijnen</name></country><country name="Royaume-Uni"><region name="Écosse"><name sortKey="Jarrett, Oswald" sort="Jarrett, Oswald" uniqKey="Jarrett O" first="Oswald" last="Jarrett">Oswald Jarrett</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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