Transcripts from a mosquito dihydrofolate reductase gene: Evidence for heterogeneity at the 5′ end
Identifieur interne : 004530 ( Main/Exploration ); précédent : 004529; suivant : 004531Transcripts from a mosquito dihydrofolate reductase gene: Evidence for heterogeneity at the 5′ end
Auteurs : Yang-Ja Park [États-Unis] ; Ann Marie Fallon [États-Unis]Source :
- Insect Biochemistry and Molecular Biology [ 0965-1748 ] ; 1993.
English descriptors
- Teeft :
- Additional bands, Aedes albopictus, Albopictus cells, Approximate locations, Bethesda research laboratories, Btol chem, Cell cycle, Coding region, Dhfr, Dhfr coding region, Dhfr gene, Dhfr mrnas, Dhfr transcripts, Different primers, Dlhydrofolate, Dominant selectable marker, Doublet, Ethanol, Extension products, Faint bands, Fallon, First exon, Functional analyses, Gene, Heterogeneity, Human dhfr transcripts, Hybridization, Hybridized, Initiation sites, Major transcript, Marie fallon, Mosquito, Mosquito dhfr gene, Mosquito dhfr transcripts, Mrna, Multiple transcription initiation sites, Northern blots, Polyadenylated, Polyadenylation signal, Present study, Primer, Primer extension, Primer extension analyses, Primer extension analysis, Primer extension experiments, Primer extensmn, Probe, Prominent bands, Promoter, Reductase, Regulatory sequences, Relative abundance, Resistant cells, Rnaase, Rnaase mapping, Rnaase mapping experiments, Schematic diagram, Show fragments, Single lntron, Tataa, Transcript, Transcription, Transcription initiation sites, Transfection vectors, Translation initiation site.
Abstract
Abstract: Heterogeneity among transcripts from the mosquito (Aedes albopictus) dihydrofolate reductase (DHFR) gene in wild-type C7-12 cells and in methotrexate-resistant Mtx-5011-128 cells has been analyzed by Northern blotting, RNAase mapping, and primer extension. In both sensitive and resistant cells, a major transcription initiation site mapped c. 11 nucleotides downstream of the TATAA box, near position —66 relative to the AUG codon. Two other major transcription initiation sites mapped approx. eight and 45 nucleotides, respectively, upstream of the TATAA box. In addition, at least six minor sites were detected, four of which mapped within TATA-like sequences. Within the AT-rich region flanking the 5′-end of the mosquito DHFR gene were four T-rich motifs and three “GTTTGTG” repeats. Additional “GTTTGTG” repeats occurred in the first exon and in the single 56 nucleotide intron of the mosquito DHFR gene. In contrast to the heterogeneity at the 5′-ends of mosquito DHFR transcripts, the 3′-end terminated at a single position, c. 22 nucleotides downstream of the polyadenylation signal.
Url:
DOI: 10.1016/0965-1748(93)90006-E
Affiliations:
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site</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Heterogeneity among transcripts from the mosquito (Aedes albopictus) dihydrofolate reductase (DHFR) gene in wild-type C7-12 cells and in methotrexate-resistant Mtx-5011-128 cells has been analyzed by Northern blotting, RNAase mapping, and primer extension. In both sensitive and resistant cells, a major transcription initiation site mapped c. 11 nucleotides downstream of the TATAA box, near position —66 relative to the AUG codon. Two other major transcription initiation sites mapped approx. eight and 45 nucleotides, respectively, upstream of the TATAA box. In addition, at least six minor sites were detected, four of which mapped within TATA-like sequences. Within the AT-rich region flanking the 5′-end of the mosquito DHFR gene were four T-rich motifs and three “GTTTGTG” repeats. Additional “GTTTGTG” repeats occurred in the first exon and in the single 56 nucleotide intron of the mosquito DHFR gene. In contrast to the heterogeneity at the 5′-ends of mosquito DHFR transcripts, the 3′-end terminated at a single position, c. 22 nucleotides downstream of the polyadenylation signal.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Park, Yang Ja" sort="Park, Yang Ja" uniqKey="Park Y" first="Yang-Ja" last="Park">Yang-Ja Park</name></noRegion><name sortKey="Fallon, Ann Marie" sort="Fallon, Ann Marie" uniqKey="Fallon A" first="Ann Marie" last="Fallon">Ann Marie Fallon</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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