MersV1, Main, Exploration, bibRecord, 004480

Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls

Identifieur interne : 004480 ( Main/Exploration ); précédent : 004479; suivant : 004481

Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls

Auteurs : K. Sainio [Finlande] ; M. Saarma [Finlande] ; D. Nonclercq [Finlande] ; L. Paulin [Finlande] ; H. Sariola [Finlande]

Source :

Cellular and Molecular Neurobiology [ 0272-4340 ] ; 1994-10-01.

RBID : ISTEX:5F2D5D5C0C3E3B218360DB4910C7D7BEEFCABBB1

English descriptors

Abstract

Summary: 1. Antisense inhibition of gene expression implies that the expression of the target protein is selectively inhibited at either the translational or the transcriptional level by complementary DNA or RNA constructs that are antiparallel to the target sequence. The antisense inhibition strategy provides means to study the roles of individual proteins and has, in spite of its limitations, gained a wide range of both therapeutic and experimental applications. 2. In developmental biology, protein expression has been selectively inhibited by the use of antisense gene transfection and by antisense deoxyoligonucleotides. The transfectability of embryonic tissues is variable, but in general fetal and embryonic cells take up foreign DNA relatively efficiently, in particular, short deoxyoligonucleotides that penetrate mesenchymal cells within a few hours without any manipulation. 3. We have now evaluated the advantages and pitfalls of antisense inhibition by deoxyoligonucleotides in organ culture and describe our experience from the inhibition of low-affinity nerve growth factor receptor expression in embryonic mouse and rat kidneys. 4. The expression of nerve growth factor receptor can be specifically inhibited by deoxyoligonucleotides, but the target sequence-dependent window of, in particular, phosphorothioate-modified oligonucleotides is quite narrow. The culture conditions affect the response to the oligonucleotides and their cellular incorporation is variable with respect to the cell type and stage of differentiation.

Url:

https://api.istex.fr/ark:/67375/1BB-J4L2DTBK-D/fulltext.pdf

DOI: 10.1007/BF02088830

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Istex, to step Corpus: 001966
to stream Istex, to step Curation: 001966
to stream Istex, to step Checkpoint: 001B17
to stream Main, to step Merge: 004543
to stream Main, to step Curation: 004480

Le document en format XML

<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls</title>
<author><name sortKey="Sainio, K" sort="Sainio, K" uniqKey="Sainio K" first="K." last="Sainio">K. Sainio</name>
</author>
<author><name sortKey="Saarma, M" sort="Saarma, M" uniqKey="Saarma M" first="M." last="Saarma">M. Saarma</name>
</author>
<author><name sortKey="Nonclercq, D" sort="Nonclercq, D" uniqKey="Nonclercq D" first="D." last="Nonclercq">D. Nonclercq</name>
</author>
<author><name sortKey="Paulin, L" sort="Paulin, L" uniqKey="Paulin L" first="L." last="Paulin">L. Paulin</name>
</author>
<author><name sortKey="Sariola, H" sort="Sariola, H" uniqKey="Sariola H" first="H." last="Sariola">H. Sariola</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:5F2D5D5C0C3E3B218360DB4910C7D7BEEFCABBB1</idno>
<date when="1994" year="1994">1994</date>
<idno type="doi">10.1007/BF02088830</idno>
<idno type="url">https://api.istex.fr/ark:/67375/1BB-J4L2DTBK-D/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001966</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001966</idno>
<idno type="wicri:Area/Istex/Curation">001966</idno>
<idno type="wicri:Area/Istex/Checkpoint">001B17</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001B17</idno>
<idno type="wicri:doubleKey">0272-4340:1994:Sainio K:antisense:inhibition:of</idno>
<idno type="wicri:Area/Main/Merge">004543</idno>
<idno type="wicri:Area/Main/Curation">004480</idno>
<idno type="wicri:Area/Main/Exploration">004480</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls</title>
<author><name sortKey="Sainio, K" sort="Sainio, K" uniqKey="Sainio K" first="K." last="Sainio">K. Sainio</name>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Department of Pathology, University of Helsinki, Haartmaninkatu 3, P.O. Box 21, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Saarma, M" sort="Saarma, M" uniqKey="Saarma M" first="M." last="Saarma">M. Saarma</name>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Institute of Biotechnology, University of Helsinki, Karvaamokuja 3, P.O. Box 45, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Nonclercq, D" sort="Nonclercq, D" uniqKey="Nonclercq D" first="D." last="Nonclercq">D. Nonclercq</name>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Department of Pathology, University of Helsinki, Haartmaninkatu 3, P.O. Box 21, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Paulin, L" sort="Paulin, L" uniqKey="Paulin L" first="L." last="Paulin">L. Paulin</name>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Institute of Biotechnology, University of Helsinki, Karvaamokuja 3, P.O. Box 45, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sariola, H" sort="Sariola, H" uniqKey="Sariola H" first="H." last="Sariola">H. Sariola</name>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Department of Pathology, University of Helsinki, Haartmaninkatu 3, P.O. Box 21, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Finlande</country>
<wicri:regionArea>Institute of Biotechnology, University of Helsinki, Karvaamokuja 3, P.O. Box 45, FIN-00014, Helsinki</wicri:regionArea>
<orgName type="university">Université d'Helsinki</orgName>
<placeName><settlement type="city">Helsinki</settlement>
<region type="région" nuts="2">Uusimaa</region>
</placeName>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Cellular and Molecular Neurobiology</title>
<title level="j" type="abbrev">Cell Mol Neurobiol</title>
<idno type="ISSN">0272-4340</idno>
<idno type="eISSN">1573-6830</idno>
<imprint><publisher>Kluwer Academic Publishers-Plenum Publishers</publisher>
<pubPlace>New York</pubPlace>
<date type="published" when="1994-10-01">1994-10-01</date>
<biblScope unit="volume">14</biblScope>
<biblScope unit="issue">5</biblScope>
<biblScope unit="page" from="439">439</biblScope>
<biblScope unit="page" to="457">457</biblScope>
</imprint>
<idno type="ISSN">0272-4340</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0272-4340</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>deoxyoligonucleotide inhibition</term>
<term>kidney morphogenesis</term>
<term>nerve growth factor receptor</term>
</keywords>
<keywords scheme="Teeft" xml:lang="en"><term>Antisense</term>
<term>Antisense inhibition</term>
<term>Antisense oligonucleotide</term>
<term>Antisense oligonucleotide inhibition experiments</term>
<term>Antisense oligonucleotides</term>
<term>Biol</term>
<term>Culture medium</term>
<term>Deoxyoligonucleotides</term>
<term>Dressier</term>
<term>Embryonic</term>
<term>Embryonic kidney</term>
<term>Embryonic kidneys</term>
<term>Embryonic metanephric kidneys</term>
<term>Embryonic mouse kidneys</term>
<term>Epithelial</term>
<term>Epithelial cells</term>
<term>Epithelial differentiation</term>
<term>Fetal calf serum</term>
<term>Grobstein</term>
<term>Helsinki</term>
<term>Hplc</term>
<term>Incorporation</term>
<term>Inductive interaction</term>
<term>Kidney</term>
<term>Kidney cultures</term>
<term>Kidney differentiation</term>
<term>Kidney mesenchyme</term>
<term>Kidney morphogenesis</term>
<term>Kidney tubules</term>
<term>Kind gifts</term>
<term>Mesenchymal</term>
<term>Mesenchymal cells</term>
<term>Mesenchyme</term>
<term>Metanephric</term>
<term>Metanephric development</term>
<term>Metanephric kidney</term>
<term>Morphogenesis</term>
<term>Mouse kidneys</term>
<term>Mouse metanephric kidneys</term>
<term>Mrna</term>
<term>Mrna levels</term>
<term>Nephrogenic</term>
<term>Nephrogenic mesenchyme</term>
<term>Nerve growth factor receptor</term>
<term>Neurotrophin receptors</term>
<term>Ngfr</term>
<term>Ngfr expression</term>
<term>Ngfr mrna</term>
<term>Ngfr protein</term>
<term>Ngfr transcript</term>
<term>Nonsense oligonucleotide</term>
<term>Nonsense oligonucleotides</term>
<term>Nucleic acids</term>
<term>Oligonucleotide</term>
<term>Oligonucleotides</term>
<term>Organ culture</term>
<term>Paulin</term>
<term>Phosphorothioate</term>
<term>Phosphorothioate deoxyoligonucleotides</term>
<term>Phosphorothioate oligonucleotides</term>
<term>Potato virus</term>
<term>Pretubular</term>
<term>Pretubular cells</term>
<term>Pretubular condensates</term>
<term>Receptor</term>
<term>Rothenpieler</term>
<term>Saarma</term>
<term>Sainio</term>
<term>Sariola</term>
<term>Target protein expression</term>
<term>Tubule</term>
<term>Unmodified</term>
<term>Unmodified oligonucleotides</term>
<term>Ureter</term>
</keywords>
</textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Summary: 1. Antisense inhibition of gene expression implies that the expression of the target protein is selectively inhibited at either the translational or the transcriptional level by complementary DNA or RNA constructs that are antiparallel to the target sequence. The antisense inhibition strategy provides means to study the roles of individual proteins and has, in spite of its limitations, gained a wide range of both therapeutic and experimental applications. 2. In developmental biology, protein expression has been selectively inhibited by the use of antisense gene transfection and by antisense deoxyoligonucleotides. The transfectability of embryonic tissues is variable, but in general fetal and embryonic cells take up foreign DNA relatively efficiently, in particular, short deoxyoligonucleotides that penetrate mesenchymal cells within a few hours without any manipulation. 3. We have now evaluated the advantages and pitfalls of antisense inhibition by deoxyoligonucleotides in organ culture and describe our experience from the inhibition of low-affinity nerve growth factor receptor expression in embryonic mouse and rat kidneys. 4. The expression of nerve growth factor receptor can be specifically inhibited by deoxyoligonucleotides, but the target sequence-dependent window of, in particular, phosphorothioate-modified oligonucleotides is quite narrow. The culture conditions affect the response to the oligonucleotides and their cellular incorporation is variable with respect to the cell type and stage of differentiation.</div>
</front>
</TEI>
<affiliations><list><country><li>Finlande</li>
</country>
<region><li>Uusimaa</li>
</region>
<settlement><li>Helsinki</li>
</settlement>
<orgName><li>Université d'Helsinki</li>
</orgName>
</list>
<tree><country name="Finlande"><region name="Uusimaa"><name sortKey="Sainio, K" sort="Sainio, K" uniqKey="Sainio K" first="K." last="Sainio">K. Sainio</name>
</region>
<name sortKey="Nonclercq, D" sort="Nonclercq, D" uniqKey="Nonclercq D" first="D." last="Nonclercq">D. Nonclercq</name>
<name sortKey="Paulin, L" sort="Paulin, L" uniqKey="Paulin L" first="L." last="Paulin">L. Paulin</name>
<name sortKey="Saarma, M" sort="Saarma, M" uniqKey="Saarma M" first="M." last="Saarma">M. Saarma</name>
<name sortKey="Sariola, H" sort="Sariola, H" uniqKey="Sariola H" first="H." last="Sariola">H. Sariola</name>
<name sortKey="Sariola, H" sort="Sariola, H" uniqKey="Sariola H" first="H." last="Sariola">H. Sariola</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004480 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004480 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    MersV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:5F2D5D5C0C3E3B218360DB4910C7D7BEEFCABBB1
   |texte=   Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls
}}

This area was generated with Dilib version V0.6.33.
Data generation: Mon Apr 20 23:26:43 2020. Site generation: Sat Mar 27 09:06:09 2021

	Serveur d'exploration MERS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration MERS

Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls

Antisense inhibition of low-affinity nerve growth factor receptor in kidney cultures: Power and pitfalls

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri