Epitope mapping
Identifieur interne : 004453 ( Main/Exploration ); précédent : 004452; suivant : 004454Epitope mapping
Auteurs : Sara E. Mole [Royaume-Uni]Source :
- Molecular Biotechnology [ 1073-6085 ] ; 1994-06-01.
English descriptors
- KwdEn :
- Teeft :
- Amino acids, Appropriate dilution, Appropriate restriction enzyme, Assay, Basic assay, Binding site, Cold spring harbor, Cold spring harbor laboratory, Competent cells, Degradation products, Deletion, Denaturing agents, Dilution series, Discontinuous epitopes, Epitope, Epitope mapping, First round, Humid environment, Hydrogen peroxide, Immunological techniques, Initial mapping, Ligation buffer, Mabs, Microtiter, Microtiter plate, Native form, Native protein, Oligonucleotides, Peptide, Peptide binding, Primary antibody, Primer, Second round, Selective media, Siliconized eppendorfs, Small fragments, Sodium acetate, Substrate solution, Substrate stock, Synthetic peptide, Third round, Typical assay.
Abstract
Abstract: This article describes a strategy for the mapping of the binding site, or epitope, of a monoclonal antibody (MAb) using bacterially expressed protein products. An overall strategy is discussed. This includes an initial round of several parallel approaches to gain the greatest amount of information at this stage. The second round uses the mapping information generated to identify MAbs, which may bind to identical or overlapping epitopes. The third round involves the design of new constructs that express small defined regions of the protein to refine the position of the epitope. The final step leads to the identification of the epitope to a resolution of 10 amino acid residues or else.
Url:
DOI: 10.1007/BF02921695
Affiliations:
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Le document en format XML
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Mole</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:C073D7781E9969D27D7C34A833C851B3EC162DAC</idno><date when="1994" year="1994">1994</date><idno type="doi">10.1007/BF02921695</idno><idno type="url">https://api.istex.fr/ark:/67375/1BB-BNR5P84P-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000C53</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000C53</idno><idno type="wicri:Area/Istex/Curation">000C53</idno><idno type="wicri:Area/Istex/Checkpoint">001A90</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001A90</idno><idno type="wicri:Area/Main/Merge">004516</idno><idno type="wicri:Area/Main/Curation">004453</idno><idno type="wicri:Area/Main/Exploration">004453</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Epitope mapping</title><author><name sortKey="Mole, Sara E" sort="Mole, Sara E" uniqKey="Mole S" first="Sara E." last="Mole">Sara E. Mole</name><affiliation wicri:level="3"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Pediatrics, University College London Medical School, The Rayne Institute, University Street, WC1E 6JJ, London</wicri:regionArea><placeName><settlement type="city">Londres</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Grand Londres</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Biotechnology</title><title level="j" type="abbrev">Mol Biotechnol</title><idno type="ISSN">1073-6085</idno><idno type="eISSN">1559-0305</idno><imprint><publisher>Humana Press</publisher><pubPlace>Totowa</pubPlace><date type="published" when="1994-06-01">1994-06-01</date><biblScope unit="volume">1</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="277">277</biblScope><biblScope unit="page" to="287">287</biblScope></imprint><idno type="ISSN">1073-6085</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1073-6085</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Epitope mapping</term><term>cloning</term><term>deletion PCR</term><term>monoclonal antibodies</term><term>oligonucleotide</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Amino acids</term><term>Appropriate dilution</term><term>Appropriate restriction enzyme</term><term>Assay</term><term>Basic assay</term><term>Binding site</term><term>Cold spring harbor</term><term>Cold spring harbor laboratory</term><term>Competent cells</term><term>Degradation products</term><term>Deletion</term><term>Denaturing agents</term><term>Dilution series</term><term>Discontinuous epitopes</term><term>Epitope</term><term>Epitope mapping</term><term>First round</term><term>Humid environment</term><term>Hydrogen peroxide</term><term>Immunological techniques</term><term>Initial mapping</term><term>Ligation buffer</term><term>Mabs</term><term>Microtiter</term><term>Microtiter plate</term><term>Native form</term><term>Native protein</term><term>Oligonucleotides</term><term>Peptide</term><term>Peptide binding</term><term>Primary antibody</term><term>Primer</term><term>Second round</term><term>Selective media</term><term>Siliconized eppendorfs</term><term>Small fragments</term><term>Sodium acetate</term><term>Substrate solution</term><term>Substrate stock</term><term>Synthetic peptide</term><term>Third round</term><term>Typical assay</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: This article describes a strategy for the mapping of the binding site, or epitope, of a monoclonal antibody (MAb) using bacterially expressed protein products. An overall strategy is discussed. This includes an initial round of several parallel approaches to gain the greatest amount of information at this stage. The second round uses the mapping information generated to identify MAbs, which may bind to identical or overlapping epitopes. The third round involves the design of new constructs that express small defined regions of the protein to refine the position of the epitope. The final step leads to the identification of the epitope to a resolution of 10 amino acid residues or else.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Grand Londres</li></region><settlement><li>Londres</li></settlement></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Mole, Sara E" sort="Mole, Sara E" uniqKey="Mole S" first="Sara E." last="Mole">Sara E. Mole</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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