Competition inhibition of cytotoxic T-lymphocyte (CTL) lysis, a more sensitive method to identify candidate CTL epitopes than induction of antibody-detected MHC class I stabilization
Identifieur interne : 004096 ( Main/Exploration ); précédent : 004095; suivant : 004097Competition inhibition of cytotoxic T-lymphocyte (CTL) lysis, a more sensitive method to identify candidate CTL epitopes than induction of antibody-detected MHC class I stabilization
Auteurs : Mariet C. W. Feltkamp [Pays-Bas] ; Michel P. M. Vierboom [Pays-Bas] ; Renée. M. Toes [Pays-Bas] ; Ferry Ossendorp [Pays-Bas] ; Jan Ter Schegget [Pays-Bas] ; Cornelis J. M. Melief [Pays-Bas] ; W. Martin Kast [Pays-Bas]Source :
- Immunology Letters [ 0165-2478 ] ; 1995.
English descriptors
- Teeft :
- Assay, Best peptide, Binding affinity, Clone, Competitor peptide, Competitor peptides, Epitope, Immunol, Kast, Length variants, Lysis, Martin kast, Melief, Mutual differences, Peptide, Reference peptide, Reference peptides, Release assay, Representative experiment, Target cells, Test peptides, Titrated, Titrated amounts.
Abstract
Abstract: We compared the efficiency of two commonly used cellular major histocompatibility complex (MHC) class I peptide-binding assays to identify a cytotoxic T lymphocyte (CTL) epitope-containing peptide among length variants derived from the human papilloma virus type 16 (HPV 16) oncoprotein E7. Although both assays identified the same sequence (E7 49–57) as the most efficient Db-binding peptide, the efficiency by which they did so differed markedly. In a peptide competition cytotoxicity (PCC) assay, based on inhibition of CTL lysis by competition for binding to MHC class-I molecules between a known CTL epitope-containing peptide and peptide of interest, E7 49–57 bound 45-fold more efficiently to Db than the second Db-binding peptide in line. In the widely used RMA-S MHC class I peptide-binding assay, based on peptide-induced stabilization of ‘empty’ MHC class-I molecules at the surface of antigen-processing defective RMA-S cells, this difference was only 3 fold. Similar differences were observed when other Db-restricted CTL clones and CTL epitope-containing peptides were used in the PCC assay. The same phenomenon was observed when peptide binding affinities for H-2Kb were analyzed in both assays. We conclude that the PCC assay discriminates more efficiently between high- and low-affinity MHC class I binding peptides than the RMA-S assay. This observation is ascribed to the fact that peptide-MHC class I dissociation is an important parameter in the PCC but not the RMA-S assay.
Url:
DOI: 10.1016/0165-2478(95)00052-7
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001590
- to stream Istex, to step Curation: 001590
- to stream Istex, to step Checkpoint: 001787
- to stream Main, to step Merge: 004157
- to stream Main, to step Curation: 004096
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Competition inhibition of cytotoxic T-lymphocyte (CTL) lysis, a more sensitive method to identify candidate CTL epitopes than induction of antibody-detected MHC class I stabilization</title><author><name sortKey="Feltkamp, Mariet C W" sort="Feltkamp, Mariet C W" uniqKey="Feltkamp M" first="Mariet C. W." last="Feltkamp">Mariet C. W. Feltkamp</name></author><author><name sortKey="Vierboom, Michel P M" sort="Vierboom, Michel P M" uniqKey="Vierboom M" first="Michel P. M." last="Vierboom">Michel P. M. Vierboom</name></author><author><name sortKey="Toes, Renee M" sort="Toes, Renee M" uniqKey="Toes R" first="Renée. M." last="Toes">Renée. M. Toes</name></author><author><name sortKey="Ossendorp, Ferry" sort="Ossendorp, Ferry" uniqKey="Ossendorp F" first="Ferry" last="Ossendorp">Ferry Ossendorp</name></author><author><name sortKey="Schegget, Jan Ter" sort="Schegget, Jan Ter" uniqKey="Schegget J" first="Jan Ter" last="Schegget">Jan Ter Schegget</name></author><author><name sortKey="Melief, Cornelis J M" sort="Melief, Cornelis J M" uniqKey="Melief C" first="Cornelis J. M." last="Melief">Cornelis J. M. Melief</name></author><author><name sortKey="Kast, W Martin" sort="Kast, W Martin" uniqKey="Kast W" first="W. Martin" last="Kast">W. Martin Kast</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:3211A87119C10C8164C254C5E028233EC869E221</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/0165-2478(95)00052-7</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-H93Q0N19-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001590</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001590</idno><idno type="wicri:Area/Istex/Curation">001590</idno><idno type="wicri:Area/Istex/Checkpoint">001787</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001787</idno><idno type="wicri:doubleKey">0165-2478:1995:Feltkamp M:competition:inhibition:of</idno><idno type="wicri:Area/Main/Merge">004157</idno><idno type="wicri:Area/Main/Curation">004096</idno><idno type="wicri:Area/Main/Exploration">004096</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Competition inhibition of cytotoxic T-lymphocyte (CTL) lysis, a more sensitive method to identify candidate CTL epitopes than induction of antibody-detected MHC class I stabilization</title><author><name sortKey="Feltkamp, Mariet C W" sort="Feltkamp, Mariet C W" uniqKey="Feltkamp M" first="Mariet C. W." last="Feltkamp">Mariet C. W. Feltkamp</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam</wicri:regionArea><wicri:noRegion>1105 AZ Amsterdam</wicri:noRegion></affiliation></author><author><name sortKey="Vierboom, Michel P M" sort="Vierboom, Michel P M" uniqKey="Vierboom M" first="Michel P. M." last="Vierboom">Michel P. M. Vierboom</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Toes, Renee M" sort="Toes, Renee M" uniqKey="Toes R" first="Renée. M." last="Toes">Renée. M. Toes</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Ossendorp, Ferry" sort="Ossendorp, Ferry" uniqKey="Ossendorp F" first="Ferry" last="Ossendorp">Ferry Ossendorp</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Schegget, Jan Ter" sort="Schegget, Jan Ter" uniqKey="Schegget J" first="Jan Ter" last="Schegget">Jan Ter Schegget</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Virology, Academic Medical Centre, Meibergdreef 15, 1105 AZ Amsterdam</wicri:regionArea><wicri:noRegion>1105 AZ Amsterdam</wicri:noRegion></affiliation></author><author><name sortKey="Melief, Cornelis J M" sort="Melief, Cornelis J M" uniqKey="Melief C" first="Cornelis J. M." last="Melief">Cornelis J. M. Melief</name><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author><author><name sortKey="Kast, W Martin" sort="Kast, W Martin" uniqKey="Kast W" first="W. Martin" last="Kast">W. Martin Kast</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Department of Immunohematology and Blood Bank, University Hospital Leiden, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea><wicri:noRegion>2300 RC Leiden</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Immunology Letters</title><title level="j" type="abbrev">IMLET</title><idno type="ISSN">0165-2478</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">47</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="1">1</biblScope><biblScope unit="page" to="8">8</biblScope></imprint><idno type="ISSN">0165-2478</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-2478</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Best peptide</term><term>Binding affinity</term><term>Clone</term><term>Competitor peptide</term><term>Competitor peptides</term><term>Epitope</term><term>Immunol</term><term>Kast</term><term>Length variants</term><term>Lysis</term><term>Martin kast</term><term>Melief</term><term>Mutual differences</term><term>Peptide</term><term>Reference peptide</term><term>Reference peptides</term><term>Release assay</term><term>Representative experiment</term><term>Target cells</term><term>Test peptides</term><term>Titrated</term><term>Titrated amounts</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We compared the efficiency of two commonly used cellular major histocompatibility complex (MHC) class I peptide-binding assays to identify a cytotoxic T lymphocyte (CTL) epitope-containing peptide among length variants derived from the human papilloma virus type 16 (HPV 16) oncoprotein E7. Although both assays identified the same sequence (E7 49–57) as the most efficient Db-binding peptide, the efficiency by which they did so differed markedly. In a peptide competition cytotoxicity (PCC) assay, based on inhibition of CTL lysis by competition for binding to MHC class-I molecules between a known CTL epitope-containing peptide and peptide of interest, E7 49–57 bound 45-fold more efficiently to Db than the second Db-binding peptide in line. In the widely used RMA-S MHC class I peptide-binding assay, based on peptide-induced stabilization of ‘empty’ MHC class-I molecules at the surface of antigen-processing defective RMA-S cells, this difference was only 3 fold. Similar differences were observed when other Db-restricted CTL clones and CTL epitope-containing peptides were used in the PCC assay. The same phenomenon was observed when peptide binding affinities for H-2Kb were analyzed in both assays. We conclude that the PCC assay discriminates more efficiently between high- and low-affinity MHC class I binding peptides than the RMA-S assay. This observation is ascribed to the fact that peptide-MHC class I dissociation is an important parameter in the PCC but not the RMA-S assay.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><country name="Pays-Bas"><noRegion><name sortKey="Feltkamp, Mariet C W" sort="Feltkamp, Mariet C W" uniqKey="Feltkamp M" first="Mariet C. W." last="Feltkamp">Mariet C. W. Feltkamp</name></noRegion><name sortKey="Feltkamp, Mariet C W" sort="Feltkamp, Mariet C W" uniqKey="Feltkamp M" first="Mariet C. W." last="Feltkamp">Mariet C. W. Feltkamp</name><name sortKey="Kast, W Martin" sort="Kast, W Martin" uniqKey="Kast W" first="W. Martin" last="Kast">W. Martin Kast</name><name sortKey="Melief, Cornelis J M" sort="Melief, Cornelis J M" uniqKey="Melief C" first="Cornelis J. M." last="Melief">Cornelis J. M. Melief</name><name sortKey="Ossendorp, Ferry" sort="Ossendorp, Ferry" uniqKey="Ossendorp F" first="Ferry" last="Ossendorp">Ferry Ossendorp</name><name sortKey="Schegget, Jan Ter" sort="Schegget, Jan Ter" uniqKey="Schegget J" first="Jan Ter" last="Schegget">Jan Ter Schegget</name><name sortKey="Toes, Renee M" sort="Toes, Renee M" uniqKey="Toes R" first="Renée. M." last="Toes">Renée. M. Toes</name><name sortKey="Vierboom, Michel P M" sort="Vierboom, Michel P M" uniqKey="Vierboom M" first="Michel P. M." last="Vierboom">Michel P. M. Vierboom</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004096 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004096 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:3211A87119C10C8164C254C5E028233EC869E221
|texte= Competition inhibition of cytotoxic T-lymphocyte (CTL) lysis, a more sensitive method to identify candidate CTL epitopes than induction of antibody-detected MHC class I stabilization
}}
| This area was generated with Dilib version V0.6.33. |  |