Structural Composition of Hammerhead Ribozymes
Identifieur interne : 004063 ( Main/Exploration ); précédent : 004062; suivant : 004064Structural Composition of Hammerhead Ribozymes
Auteurs : Akio Takenaka ; Osamu Matsumoto ; Yixin Chen ; Sei-Ichi Hasegawa ; Toshiyuki Chatake ; Masaru Tsunoda ; Tsutomu Ohta ; Yasuo Komatsu ; Makoto Koizumi ; Eiko OhtsukaSource :
- The Journal of Biochemistry [ 0021-924X ] ; 1995.
Abstract
Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains having been modified to prevent hydrolysis. Electrophoresis patterns indicated that the two strands except for the substrate chain form a binary complex and that they form a ternary complex when mixed with the substrate chain. Both complexes were crystallized. The crystal of the binary complex belongs to a Laue symmetry of 32 (space group of P321, P3121, or P3221) with cell dimensions of a=b=53.4 and c=59.4 Å. The volume per one nucleotide allows the asymmetric unit to contain one binary complex. Our results suggest that the catalytic part forms a rigid ribozyme structure which induces a scissile reaction when the substrate is bound, in a similar manner to an enzyme protein.
Url:
DOI: 10.1093/oxfordjournals.jbchem.a124786
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000551
- to stream Istex, to step Curation: 000551
- to stream Istex, to step Checkpoint: 001754
- to stream Main, to step Merge: 004124
- to stream Main, to step Curation: 004063
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Structural Composition of Hammerhead Ribozymes</title><author><name sortKey="Takenaka, Akio" sort="Takenaka, Akio" uniqKey="Takenaka A" first="Akio" last="Takenaka">Akio Takenaka</name></author><author><name sortKey="Matsumoto, Osamu" sort="Matsumoto, Osamu" uniqKey="Matsumoto O" first="Osamu" last="Matsumoto">Osamu Matsumoto</name></author><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name></author><author><name sortKey="Hasegawa, Sei Ichi" sort="Hasegawa, Sei Ichi" uniqKey="Hasegawa S" first="Sei-Ichi" last="Hasegawa">Sei-Ichi Hasegawa</name></author><author><name sortKey="Chatake, Toshiyuki" sort="Chatake, Toshiyuki" uniqKey="Chatake T" first="Toshiyuki" last="Chatake">Toshiyuki Chatake</name></author><author><name sortKey="Tsunoda, Masaru" sort="Tsunoda, Masaru" uniqKey="Tsunoda M" first="Masaru" last="Tsunoda">Masaru Tsunoda</name></author><author><name sortKey="Ohta, Tsutomu" sort="Ohta, Tsutomu" uniqKey="Ohta T" first="Tsutomu" last="Ohta">Tsutomu Ohta</name></author><author><name sortKey="Komatsu, Yasuo" sort="Komatsu, Yasuo" uniqKey="Komatsu Y" first="Yasuo" last="Komatsu">Yasuo Komatsu</name></author><author><name sortKey="Koizumi, Makoto" sort="Koizumi, Makoto" uniqKey="Koizumi M" first="Makoto" last="Koizumi">Makoto Koizumi</name></author><author><name sortKey="Ohtsuka, Eiko" sort="Ohtsuka, Eiko" uniqKey="Ohtsuka E" first="Eiko" last="Ohtsuka">Eiko Ohtsuka</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7DF77068F22665743144C6093434E3287BF033A5</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1093/oxfordjournals.jbchem.a124786</idno><idno type="url">https://api.istex.fr/ark:/67375/HXZ-FC9W3S02-7/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000551</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000551</idno><idno type="wicri:Area/Istex/Curation">000551</idno><idno type="wicri:Area/Istex/Checkpoint">001754</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001754</idno><idno type="wicri:doubleKey">0021-924X:1995:Takenaka A:structural:composition:of</idno><idno type="wicri:Area/Main/Merge">004124</idno><idno type="wicri:Area/Main/Curation">004063</idno><idno type="wicri:Area/Main/Exploration">004063</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Structural Composition of Hammerhead Ribozymes<ref type="fn" target="#fn1"><hi rend="superscript">1</hi></ref></title><author><name sortKey="Takenaka, Akio" sort="Takenaka, Akio" uniqKey="Takenaka A" first="Akio" last="Takenaka">Akio Takenaka</name><affiliation></affiliation><affiliation></affiliation><affiliation><wicri:noCountry code="no comma">2To whom correspondence should be addressed</wicri:noCountry></affiliation></author><author><name sortKey="Matsumoto, Osamu" sort="Matsumoto, Osamu" uniqKey="Matsumoto O" first="Osamu" last="Matsumoto">Osamu Matsumoto</name><affiliation><wicri:noCountry code="subField">226</wicri:noCountry></affiliation></author><author><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><affiliation><wicri:noCountry code="subField">226</wicri:noCountry></affiliation></author><author><name sortKey="Hasegawa, Sei Ichi" sort="Hasegawa, Sei Ichi" uniqKey="Hasegawa S" first="Sei-Ichi" last="Hasegawa">Sei-Ichi Hasegawa</name><affiliation><wicri:noCountry code="subField">226</wicri:noCountry></affiliation></author><author><name sortKey="Chatake, Toshiyuki" sort="Chatake, Toshiyuki" uniqKey="Chatake T" first="Toshiyuki" last="Chatake">Toshiyuki Chatake</name><affiliation><wicri:noCountry code="subField">226</wicri:noCountry></affiliation></author><author><name sortKey="Tsunoda, Masaru" sort="Tsunoda, Masaru" uniqKey="Tsunoda M" first="Masaru" last="Tsunoda">Masaru Tsunoda</name><affiliation><wicri:noCountry code="subField">226</wicri:noCountry></affiliation></author><author><name sortKey="Ohta, Tsutomu" sort="Ohta, Tsutomu" uniqKey="Ohta T" first="Tsutomu" last="Ohta">Tsutomu Ohta</name><affiliation><wicri:noCountry code="subField">060</wicri:noCountry></affiliation></author><author><name sortKey="Komatsu, Yasuo" sort="Komatsu, Yasuo" uniqKey="Komatsu Y" first="Yasuo" last="Komatsu">Yasuo Komatsu</name><affiliation><wicri:noCountry code="subField">060</wicri:noCountry></affiliation></author><author><name sortKey="Koizumi, Makoto" sort="Koizumi, Makoto" uniqKey="Koizumi M" first="Makoto" last="Koizumi">Makoto Koizumi</name><affiliation><wicri:noCountry code="subField">060</wicri:noCountry></affiliation></author><author><name sortKey="Ohtsuka, Eiko" sort="Ohtsuka, Eiko" uniqKey="Ohtsuka E" first="Eiko" last="Ohtsuka">Eiko Ohtsuka</name><affiliation><wicri:noCountry code="subField">060</wicri:noCountry></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">The Journal of Biochemistry</title><idno type="eISSN">-</idno><idno type="ISSN">0021-924X</idno><imprint><publisher>Oxford University Press</publisher><date type="published">1995</date><biblScope unit="vol">117</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="850">850</biblScope><biblScope unit="page" to="855">855</biblScope></imprint><idno type="ISSN">0021-924X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-924X</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Eleven kinds of hammerhead ribozymes were designed and synthesized to investigate the structural composition of the complexes and to find suitable crystallization conditions, the substrate chains having been modified to prevent hydrolysis. Electrophoresis patterns indicated that the two strands except for the substrate chain form a binary complex and that they form a ternary complex when mixed with the substrate chain. Both complexes were crystallized. The crystal of the binary complex belongs to a Laue symmetry of 32 (space group of P321, P3121, or P3221) with cell dimensions of a=b=53.4 and c=59.4 Å. The volume per one nucleotide allows the asymmetric unit to contain one binary complex. Our results suggest that the catalytic part forms a rigid ribozyme structure which induces a scissile reaction when the substrate is bound, in a similar manner to an enzyme protein.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Chatake, Toshiyuki" sort="Chatake, Toshiyuki" uniqKey="Chatake T" first="Toshiyuki" last="Chatake">Toshiyuki Chatake</name><name sortKey="Chen, Yixin" sort="Chen, Yixin" uniqKey="Chen Y" first="Yixin" last="Chen">Yixin Chen</name><name sortKey="Hasegawa, Sei Ichi" sort="Hasegawa, Sei Ichi" uniqKey="Hasegawa S" first="Sei-Ichi" last="Hasegawa">Sei-Ichi Hasegawa</name><name sortKey="Koizumi, Makoto" sort="Koizumi, Makoto" uniqKey="Koizumi M" first="Makoto" last="Koizumi">Makoto Koizumi</name><name sortKey="Komatsu, Yasuo" sort="Komatsu, Yasuo" uniqKey="Komatsu Y" first="Yasuo" last="Komatsu">Yasuo Komatsu</name><name sortKey="Matsumoto, Osamu" sort="Matsumoto, Osamu" uniqKey="Matsumoto O" first="Osamu" last="Matsumoto">Osamu Matsumoto</name><name sortKey="Ohta, Tsutomu" sort="Ohta, Tsutomu" uniqKey="Ohta T" first="Tsutomu" last="Ohta">Tsutomu Ohta</name><name sortKey="Ohtsuka, Eiko" sort="Ohtsuka, Eiko" uniqKey="Ohtsuka E" first="Eiko" last="Ohtsuka">Eiko Ohtsuka</name><name sortKey="Takenaka, Akio" sort="Takenaka, Akio" uniqKey="Takenaka A" first="Akio" last="Takenaka">Akio Takenaka</name><name sortKey="Tsunoda, Masaru" sort="Tsunoda, Masaru" uniqKey="Tsunoda M" first="Masaru" last="Tsunoda">Masaru Tsunoda</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004063 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004063 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:7DF77068F22665743144C6093434E3287BF033A5
|texte= Structural Composition of Hammerhead Ribozymes
}}
| This area was generated with Dilib version V0.6.33. |  |