mRNA phenotyping of the major ligands and receptors of the EGF supergene family in human ovarian epithelial cells
Identifieur interne : 004060 ( Main/Exploration ); précédent : 004059; suivant : 004061mRNA phenotyping of the major ligands and receptors of the EGF supergene family in human ovarian epithelial cells
Auteurs : Alfred W. Gordon [États-Unis] ; Joyce C. Pegues [États-Unis] ; Gibbes R. Johnson [États-Unis] ; Bhanu Kannan [États-Unis] ; Nelly Auersperg [Canada] ; Kurt Stromberg [États-Unis]Source :
- Cancer Letters [ 0304-3835 ] ; 1995.
English descriptors
- Teeft :
- Amphiregulin, Amplification, Antisense, Autocrine, Autocrine hypothesis, Cancer letters, Carcinoma, Carcinoma cell lines, Cdna, Cdna templates, Cell line, Cell lines, Cell strains, Culture conditions, Epidermal, Epidermal growth factor, Epidermal growth factor receptor, Epidermal growth factor receptor expression, Epithelial, Epithelium, Exponential curve, Final volume, Gapdh, Gapdh cdna, Glyceraldehyde dehydrogenase, Growth conditions, Growth factor, Guanidinium thiocyanate, Hybridization, Hybridization signals, Individual cell lines, Input cdna, Internal reference, Internal reference gene, Ligand, Major ligands, Mature protein, Mrna, Mrna expression, Mrna levels, Mrna phenotyping, Oligonucleotide probe, Ovarian, Ovarian cancer, Ovarian cancers, Ovarian carcinogenesis, Ovarian carcinoma cell lines, Ovarian carcinoma cells, Ovarian carcinoma lines, Ovarian surface epithelial cells, Ovca, Phenotyping, Polymerase chain reaction, Primer, Receptor, Receptor expression, Receptor mrna, Riboclone cdna synthesis system, Sample blots, Senseand antisenseprimers, Serum conditions, Serum growth conditions, Serum media, Serum ovca, Small numbers, Sonicated salmon sperm, Standard curve, Supergene family, Surface epithelium, Tumor necrosis factor.
Abstract
Abstract: mRNA amplification phenotyping (MAPPing) was used to determine the level of mRNA expression of the major EGF-related ligands (EGF, TGF-alpha, and Amphiregulin) and receptors (EGF-receptor and erbB-2) of the EGF supergene family in three ovarian carcinoma lines (OVCA 429 and 433, and NIH:OVCAR-8) under serum-supplemented and reduced serum (minimal medium with 2% fetuin) growth conditions. mRNA levels of TGF-alpha, EGF-R, and erbB-2 were particularly high, and increased approximately 2–3 orders of magnitude when grown in serum, consistent with an autocrine involvement of these genes in ovarian epithelial growth in vitro. Moreover, even when grown without serum, OVCA 429 and NIH:OVCAR-8 expressed elevated levels of mRNA for erbB-2.
Url:
DOI: 10.1016/0304-3835(95)90159-0
Affiliations:
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Auersperg</name><affiliation wicri:level="1"><country>Canada</country><wicri:regionArea>Department of Anatomy, University of British Columbia, Vancouver, B.C.</wicri:regionArea><wicri:noRegion>B.C.</wicri:noRegion></affiliation></author><author><name sortKey="Stromberg, Kurt" sort="Stromberg, Kurt" uniqKey="Stromberg K" first="Kurt" last="Stromberg">Kurt Stromberg</name><affiliation></affiliation><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Division of Cytokine Biology, Center for Biologies Research and Evaluation, Food and Drug Administration, FDA/CBER, HFM-11, Building 29A, Room 3B-20, 8800 Rockville Pike, Bethesda, MD 20892</wicri:regionArea><wicri:noRegion>MD 20892</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Cancer Letters</title><title level="j" type="abbrev">CAN</title><idno type="ISSN">0304-3835</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">89</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="63">63</biblScope><biblScope unit="page" to="71">71</biblScope></imprint><idno type="ISSN">0304-3835</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0304-3835</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Amphiregulin</term><term>Amplification</term><term>Antisense</term><term>Autocrine</term><term>Autocrine hypothesis</term><term>Cancer letters</term><term>Carcinoma</term><term>Carcinoma cell lines</term><term>Cdna</term><term>Cdna templates</term><term>Cell line</term><term>Cell lines</term><term>Cell strains</term><term>Culture conditions</term><term>Epidermal</term><term>Epidermal growth factor</term><term>Epidermal growth factor receptor</term><term>Epidermal growth factor receptor expression</term><term>Epithelial</term><term>Epithelium</term><term>Exponential curve</term><term>Final volume</term><term>Gapdh</term><term>Gapdh cdna</term><term>Glyceraldehyde dehydrogenase</term><term>Growth conditions</term><term>Growth factor</term><term>Guanidinium thiocyanate</term><term>Hybridization</term><term>Hybridization signals</term><term>Individual cell lines</term><term>Input cdna</term><term>Internal reference</term><term>Internal reference gene</term><term>Ligand</term><term>Major ligands</term><term>Mature protein</term><term>Mrna</term><term>Mrna expression</term><term>Mrna levels</term><term>Mrna phenotyping</term><term>Oligonucleotide probe</term><term>Ovarian</term><term>Ovarian cancer</term><term>Ovarian cancers</term><term>Ovarian carcinogenesis</term><term>Ovarian carcinoma cell lines</term><term>Ovarian carcinoma cells</term><term>Ovarian carcinoma lines</term><term>Ovarian surface epithelial cells</term><term>Ovca</term><term>Phenotyping</term><term>Polymerase chain reaction</term><term>Primer</term><term>Receptor</term><term>Receptor expression</term><term>Receptor mrna</term><term>Riboclone cdna synthesis system</term><term>Sample blots</term><term>Senseand antisenseprimers</term><term>Serum conditions</term><term>Serum growth conditions</term><term>Serum media</term><term>Serum ovca</term><term>Small numbers</term><term>Sonicated salmon sperm</term><term>Standard curve</term><term>Supergene family</term><term>Surface epithelium</term><term>Tumor necrosis factor</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: mRNA amplification phenotyping (MAPPing) was used to determine the level of mRNA expression of the major EGF-related ligands (EGF, TGF-alpha, and Amphiregulin) and receptors (EGF-receptor and erbB-2) of the EGF supergene family in three ovarian carcinoma lines (OVCA 429 and 433, and NIH:OVCAR-8) under serum-supplemented and reduced serum (minimal medium with 2% fetuin) growth conditions. mRNA levels of TGF-alpha, EGF-R, and erbB-2 were particularly high, and increased approximately 2–3 orders of magnitude when grown in serum, consistent with an autocrine involvement of these genes in ovarian epithelial growth in vitro. Moreover, even when grown without serum, OVCA 429 and NIH:OVCAR-8 expressed elevated levels of mRNA for erbB-2.</div></front></TEI><affiliations><list><country><li>Canada</li><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Gordon, Alfred W" sort="Gordon, Alfred W" uniqKey="Gordon A" first="Alfred W." last="Gordon">Alfred W. Gordon</name></noRegion><name sortKey="Johnson, Gibbes R" sort="Johnson, Gibbes R" uniqKey="Johnson G" first="Gibbes R." last="Johnson">Gibbes R. Johnson</name><name sortKey="Kannan, Bhanu" sort="Kannan, Bhanu" uniqKey="Kannan B" first="Bhanu" last="Kannan">Bhanu Kannan</name><name sortKey="Pegues, Joyce C" sort="Pegues, Joyce C" uniqKey="Pegues J" first="Joyce C." last="Pegues">Joyce C. Pegues</name><name sortKey="Stromberg, Kurt" sort="Stromberg, Kurt" uniqKey="Stromberg K" first="Kurt" last="Stromberg">Kurt Stromberg</name></country><country name="Canada"><noRegion><name sortKey="Auersperg, Nelly" sort="Auersperg, Nelly" uniqKey="Auersperg N" first="Nelly" last="Auersperg">Nelly Auersperg</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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