MersV1, Main, Exploration, bibRecord, 004056

Analysis of modified oligonucleotides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry.

Identifieur interne : 004056 ( Main/Exploration ); précédent : 004055; suivant : 004057

Analysis of modified oligonucleotides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry.

Auteurs : E A Stemmler [États-Unis] ; M V Buchanan ; G B Hurst ; R L Hettich

Source :

Analytical chemistry [ 0003-2700 ] ; 1995.

RBID : pubmed:8779417

Descripteurs français

KwdFr :
- Analyse de Fourier, Analyse de séquence d'ADN (), Cyclotrons, Données de séquences moléculaires, Masse moléculaire, Oligonucléotides (analyse), Spectrométrie de masse MALDI, Séquence nucléotidique.
MESH :
- analyse : Oligonucléotides.
- Analyse de Fourier, Analyse de séquence d'ADN, Cyclotrons, Données de séquences moléculaires, Masse moléculaire, Spectrométrie de masse MALDI, Séquence nucléotidique.

English descriptors

KwdEn :
- Base Sequence, Cyclotrons, Fourier Analysis, Molecular Sequence Data, Molecular Weight, Oligonucleotides (analysis), Sequence Analysis, DNA (methods), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
MESH :
- chemical , analysis : Oligonucleotides.
- methods : Sequence Analysis, DNA.
- Base Sequence, Cyclotrons, Fourier Analysis, Molecular Sequence Data, Molecular Weight, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.

Abstract

Matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance mass spectrometry (FTMS) has been applied to the structural characterization of modified oligodeoxyribonucleotide 4-, 6-, and 11-mers. Each oligonucleotide contained one modified base, either an O6-methyl-substituted guanine, an N6-(10R)-trans-opened benzo[a]pyrenediol epoxide adduct of adenine, or an N2-(R)-styrene oxide adduct of guanine. 3-Hydroxypicolinic acid was used as the MALDI matrix for molecular weight and purity determinations, while either 2,5-dihydroxybenzoic acid (DHBA) or an anthranilic/nicotinic acid (AA/NA) mixture was used to induce fragmentation for the production of structurally significant fragment ions. For the 4- and 6-mers, the oligonucleotide sequence could be obtained from the direct AA/NA or DHBA spectra. Sequence information was also obtained by inserting a time delay between the laser desorption event and ion detection to permit metastable decomposition. For the 11-mers, high-mass sequence ions were not detected. Although similar sequence ions were observed in both the positive and the negative ion mass spectra, more fragmentation was generally observed in the positive ion mode. In the positive ion mode, modified base fragment ions were observed when DHBA was used, and these fragments were examined using accurate mass measurements, collisionally induced dissociations, and ion-molecule reactions to characterize the modified base. MALDI-FTMS signals from one sample application can be used for the measurement of hundreds of spectra. The direct MALDI-FT mass spectra show matrix-dependent, structurally informative fragments, and CID experiments can be implemented using low-picomole sample quantities.

DOI: 10.1021/ac00113a029
PubMed: 8779417

Affiliations:

États-Unis

Le document en format XML

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<front><div type="abstract" xml:lang="en">Matrix-assisted laser desorption/ionization (MALDI) Fourier transform ion cyclotron resonance mass spectrometry (FTMS) has been applied to the structural characterization of modified oligodeoxyribonucleotide 4-, 6-, and 11-mers. Each oligonucleotide contained one modified base, either an O6-methyl-substituted guanine, an N6-(10R)-trans-opened benzo[a]pyrenediol epoxide adduct of adenine, or an N2-(R)-styrene oxide adduct of guanine. 3-Hydroxypicolinic acid was used as the MALDI matrix for molecular weight and purity determinations, while either 2,5-dihydroxybenzoic acid (DHBA) or an anthranilic/nicotinic acid (AA/NA) mixture was used to induce fragmentation for the production of structurally significant fragment ions. For the 4- and 6-mers, the oligonucleotide sequence could be obtained from the direct AA/NA or DHBA spectra. Sequence information was also obtained by inserting a time delay between the laser desorption event and ion detection to permit metastable decomposition. For the 11-mers, high-mass sequence ions were not detected. Although similar sequence ions were observed in both the positive and the negative ion mass spectra, more fragmentation was generally observed in the positive ion mode. In the positive ion mode, modified base fragment ions were observed when DHBA was used, and these fragments were examined using accurate mass measurements, collisionally induced dissociations, and ion-molecule reactions to characterize the modified base. MALDI-FTMS signals from one sample application can be used for the measurement of hundreds of spectra. The direct MALDI-FT mass spectra show matrix-dependent, structurally informative fragments, and CID experiments can be implemented using low-picomole sample quantities.</div>
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Analysis of modified oligonucleotides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry.

Analysis of modified oligonucleotides by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

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