Identification of Mutant Adeno-Associated Virus Rep Proteins Which Are Dominant-Negative for DNA Helicase Activity
Identifieur interne : 003F62 ( Main/Exploration ); précédent : 003F61; suivant : 003F63Identification of Mutant Adeno-Associated Virus Rep Proteins Which Are Dominant-Negative for DNA Helicase Activity
Auteurs : Sirkka R. M. Kyöstiö ; Roland A. OwensSource :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 1996.
English descriptors
- Teeft :
Abstract
Abstract: Adeno-associated virus type 2 (AAV) Rep proteins have been postulated to play a role in unwinding the 145-bp inverted terminal repeats during AAV DNA replication. Previous studies showed that AAV Rep78 and Rep68 could unwind a DNA partial duplex of 26 bp. In this work it is demonstrated that nuclear extracts of human 293 cells containing wild-type Rep68 can unwind partial DNA duplexes up to 160 bp long. Mutant Rep proteins with either a histidine substituted for lysine 340 or a deletion of methionine 225 had no detectable helicase activity and inhibited the helicase activity of wild-type Rep68 protein. This observation is consistent with the model that the functional form of the Rep proteins is a multimer.
Url:
DOI: 10.1006/bbrc.1996.0399
Affiliations:
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