Intracellular Oligomerization of Influenza Virus Nucleoprotein
Identifieur interne : 003F50 ( Main/Exploration ); précédent : 003F49; suivant : 003F51Intracellular Oligomerization of Influenza Virus Nucleoprotein
Auteurs : E. N. Prokudina-Kantorovich [Russie] ; N. P. Semenova [Russie]Source :
- Virology [ 0042-6822 ] ; 1996.
English descriptors
- Teeft :
- Acrylamide concentration, Aqueous phase, Becht, Cell lysate, Cell surface, Culture medium, Detergent phase, Different influenza virus strains, Different strains, Extracellular, Extracellular medium, Hydrophobic properties, Influenza, Influenza virus, Influenza virus nucleoprotein, Influenza viruses, Intracellular, Laemmli sample buffer, Lysate, Mabs, Mdck, Mdck cells, Monomer, Monomeric, Oligomeric, Oligomerization, Oligomers, Partitioning, Present report, Semenova, Soluble fractions, Triton, Unboiled, Unboiled samples, Virol, Virology, Virus, Vivo oligomerization.
Abstract
Abstract: It has previously been shown that the purified influenza virus nucleoprotein (NP) forms the oligomersin vitroin NP preparations obtained from virions (Wileyet al.,1977,Virology,79, 446–448; Ruigrok and Baudin, 1995,J. Gen. Virol.,76, 1009–1014) and infected cells (Becht and Weiss, 1991, Behring Inst. Mitt., Justus-Liebig Universitat, Giessen, 89, 1–11). We have shown in this report that boiling-sensitive NP oligomers (di- and trimers) are formedin vivoin the course of intracellular influenza virus replication. They are detected by PAGE about 10 min after monomeric 56-kDa NP molecules are synthesized. NP oligomers are formed by different strains of influenza virus in different cell lines. Some influenza virus strains are characterized by complete conversion of NP monomers into oligomers and others by only partial conversion. In the Triton X-114 phase partitioning system NP oligomers show more hydrophobicity than NP monomers. NP oligomers are detected in the sedimentable and soluble fractions of both cell lysate and extracellular medium. The possibility is discussed that oligomeric NP is a native and functionally significant form of influenza virus NP.
Url:
DOI: 10.1006/viro.1996.0454
Affiliations:
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N." last="Prokudina-Kantorovich">E. N. Prokudina-Kantorovich</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>The D. I. Ivanovsky Institute of Virology, the Academy of Medical Sciences, Moscow, 123098</wicri:regionArea><wicri:noRegion>123098</wicri:noRegion></affiliation></author><author><name sortKey="Semenova, N P" sort="Semenova, N P" uniqKey="Semenova N" first="N. P." last="Semenova">N. P. Semenova</name><affiliation wicri:level="1"><country xml:lang="fr">Russie</country><wicri:regionArea>The D. I. Ivanovsky Institute of Virology, the Academy of Medical Sciences, Moscow, 123098</wicri:regionArea><wicri:noRegion>123098</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1996">1996</date><biblScope unit="volume">223</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="51">51</biblScope><biblScope unit="page" to="56">56</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acrylamide concentration</term><term>Aqueous phase</term><term>Becht</term><term>Cell lysate</term><term>Cell surface</term><term>Culture medium</term><term>Detergent phase</term><term>Different influenza virus strains</term><term>Different strains</term><term>Extracellular</term><term>Extracellular medium</term><term>Hydrophobic properties</term><term>Influenza</term><term>Influenza virus</term><term>Influenza virus nucleoprotein</term><term>Influenza viruses</term><term>Intracellular</term><term>Laemmli sample buffer</term><term>Lysate</term><term>Mabs</term><term>Mdck</term><term>Mdck cells</term><term>Monomer</term><term>Monomeric</term><term>Oligomeric</term><term>Oligomerization</term><term>Oligomers</term><term>Partitioning</term><term>Present report</term><term>Semenova</term><term>Soluble fractions</term><term>Triton</term><term>Unboiled</term><term>Unboiled samples</term><term>Virol</term><term>Virology</term><term>Virus</term><term>Vivo oligomerization</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: It has previously been shown that the purified influenza virus nucleoprotein (NP) forms the oligomersin vitroin NP preparations obtained from virions (Wileyet al.,1977,Virology,79, 446–448; Ruigrok and Baudin, 1995,J. Gen. Virol.,76, 1009–1014) and infected cells (Becht and Weiss, 1991, Behring Inst. Mitt., Justus-Liebig Universitat, Giessen, 89, 1–11). We have shown in this report that boiling-sensitive NP oligomers (di- and trimers) are formedin vivoin the course of intracellular influenza virus replication. They are detected by PAGE about 10 min after monomeric 56-kDa NP molecules are synthesized. NP oligomers are formed by different strains of influenza virus in different cell lines. Some influenza virus strains are characterized by complete conversion of NP monomers into oligomers and others by only partial conversion. In the Triton X-114 phase partitioning system NP oligomers show more hydrophobicity than NP monomers. NP oligomers are detected in the sedimentable and soluble fractions of both cell lysate and extracellular medium. The possibility is discussed that oligomeric NP is a native and functionally significant form of influenza virus NP.</div></front></TEI><affiliations><list><country><li>Russie</li></country></list><tree><country name="Russie"><noRegion><name sortKey="Prokudina Kantorovich, E N" sort="Prokudina Kantorovich, E N" uniqKey="Prokudina Kantorovich E" first="E. N." last="Prokudina-Kantorovich">E. N. Prokudina-Kantorovich</name></noRegion><name sortKey="Semenova, N P" sort="Semenova, N P" uniqKey="Semenova N" first="N. P." last="Semenova">N. P. Semenova</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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