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Novel members of a family of AT hook-containing DNA-binding proteins from rice are identified through their in vitro interaction with consensus target sites of plant and animal homeodomain proteins

Identifieur interne : 003F39 ( Main/Exploration ); précédent : 003F38; suivant : 003F40

Novel members of a family of AT hook-containing DNA-binding proteins from rice are identified through their in vitro interaction with consensus target sites of plant and animal homeodomain proteins

Auteurs : Annemarie H. Meijer [Pays-Bas] ; Erwin L. Van Dijk [Pays-Bas] ; J. Harry C. Hoge [Pays-Bas]

Source :

RBID : ISTEX:5B43E40330EBBDB558AA248A48EAF1C6FB45CC39

English descriptors

Abstract

Abstract: The AT hook is an AT-rich DNA-binding domain that occurs three times in mammalian high-mobility-group I/Y chromosomal proteins and has recently also been identified in DNA-binding proteins from plants. We unexpectedly isolated three rice cDNA clones encoding AT hook-containing proteins in an attempt to isolate homeobox cDNA clones by south-western screening of an expression library with known binding sites forArabidopsis and animal homeodomain proteins. One of these clones (Os-PF1) has previously been identified due to the binding of its encoded protein to PE1, acis-acting element from the oat phytochrome promoter. The other two clones represent rewly described cDNA clones, designatedOs-AT1 andOs-AT2. The Os-AT1 and Os-AT2 proteins were found to have the same specificities as Os-PF1 with respect toin vitro binding of wild-type and mutant PE1 versions. However, all three proteins appeared to bind much stronger in south-western assays to two of the rather AT-rich sequences used in our screening than to the PE1 element. In none of the AT hook proteins clear homologies to transcriptional activation domains could be identified, but the N-terminal regions of Os-AT1 and Os-PF1 were found to show similarity to histone H1 chromosomal proteins. Given their structural characteristics it is conceivable that the rice AT hook proteins bind to gene promoter regions as accessory proteins that may alter the accessibility of chromatin to other nuclear factors. Their predominant expression in young and meristematic tissues suggests that the presence of the AT hook proteins may affect the expression of genes that determine the differentiation status of cells.

Url:
DOI: 10.1007/BF00042233


Affiliations:


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Le document en format XML

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<term>rice</term>
<term>transcription factor</term>
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<term>Accessory proteins</term>
<term>Amino</term>
<term>Amino acid protein</term>
<term>Amino acid sequences</term>
<term>Amino acids</term>
<term>Animal homeodomain proteins</term>
<term>Arabidopsis</term>
<term>Arabidopsis athb</term>
<term>Assay</term>
<term>Assay conditions</term>
<term>Base pairs</term>
<term>Binding characteristics</term>
<term>Binding protein</term>
<term>Binding reaction</term>
<term>Binding site</term>
<term>Binding sites</term>
<term>Biol chem</term>
<term>Cdna</term>
<term>Cdna clone</term>
<term>Cdna clones</term>
<term>Cdna expression library</term>
<term>Cdna library</term>
<term>Cell suspension culture</term>
<term>Cell suspension cultures</term>
<term>Chromosomal</term>
<term>Chromosomal proteins</term>
<term>Clone</term>
<term>Complementary oligonucleotides</term>
<term>Concatenated probes</term>
<term>Control protein</term>
<term>Core sequence</term>
<term>Denatured herring sperm</term>
<term>Different stages</term>
<term>Differentiation status</term>
<term>Drosophila</term>
<term>Drosophila cdna expression library</term>
<term>Embl accession number</term>
<term>Embryo</term>
<term>Encoding</term>
<term>Etiolated seedlings</term>
<term>Expression assay</term>
<term>Expression library</term>
<term>Expression patterns</term>
<term>Filter binding assay</term>
<term>Filter binding assays</term>
<term>Flowering plants</term>
<term>Gene promoter regions</term>
<term>Gene regulation</term>
<term>Genes devel</term>
<term>High degree</term>
<term>High percentage</term>
<term>Histone</term>
<term>Hmgy consensus</term>
<term>Homeobox</term>
<term>Homeobox cdna clones</term>
<term>Homeobox cdnas</term>
<term>Homeodomain</term>
<term>Homeodomain protein binding sites</term>
<term>Homeodomain protein recognition sites</term>
<term>Homeodomain proteins</term>
<term>Hook</term>
<term>Hook motif</term>
<term>Hook motifs</term>
<term>Hook protein</term>
<term>Hook proteins</term>
<term>Hook proteins bind</term>
<term>Immature embryos</term>
<term>Immature zygotic embryos</term>
<term>Indica</term>
<term>Indica rice</term>
<term>Japonica rice taipei</term>
<term>Library screening</term>
<term>Lysine residues</term>
<term>Mature plants</term>
<term>Meristematic tissues</term>
<term>Milk powder</term>
<term>Minor groove</term>
<term>Mobility group</term>
<term>Mobility group protein</term>
<term>Mrna</term>
<term>Mrna level</term>
<term>Mrna levels</term>
<term>Mrna size</term>
<term>Mutant</term>
<term>Nitrocellulose filters</term>
<term>Northern blots</term>
<term>Nucl acids</term>
<term>Nuclear factor</term>
<term>Nuclear factors</term>
<term>Oryza sativa</term>
<term>Other proteins</term>
<term>Phage encoding</term>
<term>Phya3</term>
<term>Phya3 promoter</term>
<term>Phytochrome</term>
<term>Phytochrome phya3 promoter element</term>
<term>Phytochrome promoter element</term>
<term>Plant cell</term>
<term>Plant promoters</term>
<term>Plant proteins</term>
<term>Positive clones</term>
<term>Positive factor</term>
<term>Positive plaques</term>
<term>Probe</term>
<term>Probe labelling intensity</term>
<term>Proc natl acad</term>
<term>Proline residues</term>
<term>Promoter</term>
<term>Protein</term>
<term>Regeneration</term>
<term>Regeneration medium</term>
<term>Reporter gene</term>
<term>Rice cdna clones</term>
<term>Rice cdna clones encoding</term>
<term>Rice proteins</term>
<term>Rice rice</term>
<term>Room temperature</term>
<term>Screening</term>
<term>Scutellum callus</term>
<term>Seedling</term>
<term>Sequence analysis</term>
<term>Sequence specificity</term>
<term>Similar affinity</term>
<term>Sodium phosphate</term>
<term>Somatic</term>
<term>Somatic embryos</term>
<term>Structural feature</term>
<term>Structural features</term>
<term>Tobacco protein</term>
<term>Transcript level</term>
<term>Transcript levels</term>
<term>Transcription</term>
<term>Transcription factor</term>
<term>Transcription factors</term>
<term>Transcriptional</term>
<term>Transgenic plants</term>
<term>Undifferentiated</term>
<term>Undifferentiated cells</term>
<term>Unpublished results</term>
<term>Various rice tissues</term>
<term>Various tissues</term>
<term>Virus induction</term>
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<div type="abstract" xml:lang="en">Abstract: The AT hook is an AT-rich DNA-binding domain that occurs three times in mammalian high-mobility-group I/Y chromosomal proteins and has recently also been identified in DNA-binding proteins from plants. We unexpectedly isolated three rice cDNA clones encoding AT hook-containing proteins in an attempt to isolate homeobox cDNA clones by south-western screening of an expression library with known binding sites forArabidopsis and animal homeodomain proteins. One of these clones (Os-PF1) has previously been identified due to the binding of its encoded protein to PE1, acis-acting element from the oat phytochrome promoter. The other two clones represent rewly described cDNA clones, designatedOs-AT1 andOs-AT2. The Os-AT1 and Os-AT2 proteins were found to have the same specificities as Os-PF1 with respect toin vitro binding of wild-type and mutant PE1 versions. However, all three proteins appeared to bind much stronger in south-western assays to two of the rather AT-rich sequences used in our screening than to the PE1 element. In none of the AT hook proteins clear homologies to transcriptional activation domains could be identified, but the N-terminal regions of Os-AT1 and Os-PF1 were found to show similarity to histone H1 chromosomal proteins. Given their structural characteristics it is conceivable that the rice AT hook proteins bind to gene promoter regions as accessory proteins that may alter the accessibility of chromatin to other nuclear factors. Their predominant expression in young and meristematic tissues suggests that the presence of the AT hook proteins may affect the expression of genes that determine the differentiation status of cells.</div>
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