Analysis of DNA fragmentation using a dynamic size-sieving polymer solution in capillary electrophoresis
Identifieur interne : 003D95 ( Main/Exploration ); précédent : 003D94; suivant : 003D96Analysis of DNA fragmentation using a dynamic size-sieving polymer solution in capillary electrophoresis
Auteurs : Barbara A. Siles [États-Unis] ; Zeena E. Nackerdien [États-Unis] ; G. Bruce Collier [États-Unis]Source :
- Journal of Chromatography A [ 0021-9673 ] ; 1997.
Descripteurs français
- Wicri :
- topic : ADN.
English descriptors
- KwdEn :
- Teeft :
- Absorbance spectroscopy, Apoptosis, Apoptotic, Aqueous buffer medium, Average size, Base pair assignments, Base pairs, Broad distribution, Calibration curve, Capillary electrophoresis, Capillary length, Cell death, Cell destruction, Cell type, Cellular chromatin, Chromatogr, Constant time period, Degradation, Degradation products, Digestion, Digestion products, Electrophoretic, Electrophoretic migration, Electrophoretic separation, Enzymatic digestion, Error bars, Field strength, Fragment, Fragment length, Fragment size, Fragment sizes, Fragmentation, Higher multiples, Human leukemia cells, Hydroxyethyl cellulose, Larger fragments, Matrix solution, Micrococcal nuclease, Migration time precision, Migration times, Minute digestion, Multiple injections, National institute, Nuclease, Nucleosomal, Peak area, Peak area values, Peak assignments, Peak broadness, Polymer solution, Protein contamination, Radiation doses, Reproducibility, Reproducibility study, Second degree polynomial curve, Separation conditions, Separation matrix, Significant peak, Siles, Size range, Smaller fragments, Standard deviations, Standard fragments, Tacs apoptotic, Time periods, Unexposed, Whole cell.
Abstract
Abstract: Various natural and induced processes cause DNA fragmentation. Examples of these processes include apoptosis, enzymatic digestion, free radical production from ionizing radiation, photoscission by laser radiation and thermal degradation. Slab gel electrophoresis has been used most often to monitor such DNA damage. We have investigated with capillary electrophoresis the use of a new size-sieving polymer solution, TreviSol-CE (TS-CE), to monitor the DNA fragments produced from a variety of degradation processes. This polymer solution provides high run-to-run migration time and peak width reproducibilities and high separation efficiency of double-stranded DNA fragments in the 500 to 7000 base pair size range. Analysis of apoptotic DNA fragments suggested the presence of multiple nucleosomes within each cell type investigated. For irradiated DNA standards, peak-width-at-half-height and peak area were used to monitor the progress of DNA fragmentation. For both apoptotic DNA and irradiated DNA standards, fine structural features of fragmentation were revealed.
Url:
DOI: 10.1016/S0021-9673(97)00050-2
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000907
- to stream Istex, to step Curation: 000907
- to stream Istex, to step Checkpoint: 001572
- to stream Main, to step Merge: 003E50
- to stream Main, to step Curation: 003D95
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Analysis of DNA fragmentation using a dynamic size-sieving polymer solution in capillary electrophoresis</title><author><name sortKey="Siles, Barbara A" sort="Siles, Barbara A" uniqKey="Siles B" first="Barbara A." last="Siles">Barbara A. Siles</name></author><author><name sortKey="Nackerdien, Zeena E" sort="Nackerdien, Zeena E" uniqKey="Nackerdien Z" first="Zeena E." last="Nackerdien">Zeena E. Nackerdien</name></author><author><name sortKey="Bruce Collier, G" sort="Bruce Collier, G" uniqKey="Bruce Collier G" first="G." last="Bruce Collier">G. Bruce Collier</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:1ED9F2CBCFB7832300B6D0A4274550D019B3754F</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1016/S0021-9673(97)00050-2</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-F58SNMBV-J/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000907</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000907</idno><idno type="wicri:Area/Istex/Curation">000907</idno><idno type="wicri:Area/Istex/Checkpoint">001572</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001572</idno><idno type="wicri:doubleKey">0021-9673:1997:Siles B:analysis:of:dna</idno><idno type="wicri:Area/Main/Merge">003E50</idno><idno type="wicri:Area/Main/Curation">003D95</idno><idno type="wicri:Area/Main/Exploration">003D95</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Analysis of DNA fragmentation using a dynamic size-sieving polymer solution in capillary electrophoresis</title><author><name sortKey="Siles, Barbara A" sort="Siles, Barbara A" uniqKey="Siles B" first="Barbara A." last="Siles">Barbara A. Siles</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899</wicri:regionArea><wicri:noRegion>MD 20899</wicri:noRegion></affiliation></author><author><name sortKey="Nackerdien, Zeena E" sort="Nackerdien, Zeena E" uniqKey="Nackerdien Z" first="Zeena E." last="Nackerdien">Zeena E. Nackerdien</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biotechnology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899</wicri:regionArea><wicri:noRegion>MD 20899</wicri:noRegion></affiliation></author><author><name sortKey="Bruce Collier, G" sort="Bruce Collier, G" uniqKey="Bruce Collier G" first="G." last="Bruce Collier">G. Bruce Collier</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Trevigen, Inc. 8405 Helgerman Court, Gaithersburg, MD 20877</wicri:regionArea><wicri:noRegion>MD 20877</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Chromatography A</title><title level="j" type="abbrev">CHROMA</title><idno type="ISSN">0021-9673</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">771</biblScope><biblScope unit="issue">1–2</biblScope><biblScope unit="page" from="319">319</biblScope><biblScope unit="page" to="329">329</biblScope></imprint><idno type="ISSN">0021-9673</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9673</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Buffer composition</term><term>DNA</term><term>DNA fragmentation</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Absorbance spectroscopy</term><term>Apoptosis</term><term>Apoptotic</term><term>Aqueous buffer medium</term><term>Average size</term><term>Base pair assignments</term><term>Base pairs</term><term>Broad distribution</term><term>Calibration curve</term><term>Capillary electrophoresis</term><term>Capillary length</term><term>Cell death</term><term>Cell destruction</term><term>Cell type</term><term>Cellular chromatin</term><term>Chromatogr</term><term>Constant time period</term><term>Degradation</term><term>Degradation products</term><term>Digestion</term><term>Digestion products</term><term>Electrophoretic</term><term>Electrophoretic migration</term><term>Electrophoretic separation</term><term>Enzymatic digestion</term><term>Error bars</term><term>Field strength</term><term>Fragment</term><term>Fragment length</term><term>Fragment size</term><term>Fragment sizes</term><term>Fragmentation</term><term>Higher multiples</term><term>Human leukemia cells</term><term>Hydroxyethyl cellulose</term><term>Larger fragments</term><term>Matrix solution</term><term>Micrococcal nuclease</term><term>Migration time precision</term><term>Migration times</term><term>Minute digestion</term><term>Multiple injections</term><term>National institute</term><term>Nuclease</term><term>Nucleosomal</term><term>Peak area</term><term>Peak area values</term><term>Peak assignments</term><term>Peak broadness</term><term>Polymer solution</term><term>Protein contamination</term><term>Radiation doses</term><term>Reproducibility</term><term>Reproducibility study</term><term>Second degree polynomial curve</term><term>Separation conditions</term><term>Separation matrix</term><term>Significant peak</term><term>Siles</term><term>Size range</term><term>Smaller fragments</term><term>Standard deviations</term><term>Standard fragments</term><term>Tacs apoptotic</term><term>Time periods</term><term>Unexposed</term><term>Whole cell</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>ADN</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Various natural and induced processes cause DNA fragmentation. Examples of these processes include apoptosis, enzymatic digestion, free radical production from ionizing radiation, photoscission by laser radiation and thermal degradation. Slab gel electrophoresis has been used most often to monitor such DNA damage. We have investigated with capillary electrophoresis the use of a new size-sieving polymer solution, TreviSol-CE (TS-CE), to monitor the DNA fragments produced from a variety of degradation processes. This polymer solution provides high run-to-run migration time and peak width reproducibilities and high separation efficiency of double-stranded DNA fragments in the 500 to 7000 base pair size range. Analysis of apoptotic DNA fragments suggested the presence of multiple nucleosomes within each cell type investigated. For irradiated DNA standards, peak-width-at-half-height and peak area were used to monitor the progress of DNA fragmentation. For both apoptotic DNA and irradiated DNA standards, fine structural features of fragmentation were revealed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Siles, Barbara A" sort="Siles, Barbara A" uniqKey="Siles B" first="Barbara A." last="Siles">Barbara A. Siles</name></noRegion><name sortKey="Bruce Collier, G" sort="Bruce Collier, G" uniqKey="Bruce Collier G" first="G." last="Bruce Collier">G. Bruce Collier</name><name sortKey="Nackerdien, Zeena E" sort="Nackerdien, Zeena E" uniqKey="Nackerdien Z" first="Zeena E." last="Nackerdien">Zeena E. Nackerdien</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003D95 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003D95 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:1ED9F2CBCFB7832300B6D0A4274550D019B3754F
|texte= Analysis of DNA fragmentation using a dynamic size-sieving polymer solution in capillary electrophoresis
}}
| This area was generated with Dilib version V0.6.33. |  |