MersV1, Main, Exploration, bibRecord, 003D47

Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Identifieur interne : 003D47 ( Main/Exploration ); précédent : 003D46; suivant : 003D48

Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Auteurs : Y. Shoyama [Japon] ; X. X. Zhu [Japon] ; R. Nakai [Japon] ; S. Shiraishi [Japon] ; H. Kohda [Japon]

Source :

Plant Cell Reports [ 0721-7714 ] ; 1997-04-01.

RBID : ISTEX:41E7CA434355CCD0AC68346106D6B64E026F4A4A

English descriptors

KwdEn :
- Genetic homogeneity, Micropropagation, Panax notoginseng, RAPD analysis.

Abstract

Abstract: Somatic embryogenesis was induced in callus tissues derived from young flower buds ofPanax notoginseng via callus within 18 weeks of culture. The mature somatic embryos were germinated on half-strength Murashige and Skoog's (MS) medium supplemented with gibberellic acid A3(GA) and 6-benzyladenine (BA). The most suitable medium for optimal root formation proved to be MS medium supplemented with 1-naphthaleneacetic acid (NAA). Total DNA was extracted from the leaves of the regenerated plantlets ofP. notoginseng. Analysis of random-amplified polymorphic DNA (RAPD) using 21 arbitrary oligonucleotide 10-mers, showed the genetic homogeneity ofP. notoginseng. The amplification products were monomorphic for all of the plantlets ofP. notoginseng regenerated by embryogenesis, suggesting that somatic embryogenesis can be used for clonal micropropagation of this plant.

Url:

https://api.istex.fr/ark:/67375/VQC-VBKGL6VR-T/fulltext.pdf

DOI: 10.1007/BF01092764

Affiliations:

Le document en format XML

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Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Micropropagation of Panax notoginseng by somatic embryogenesis and RAPD analysis of regenerated plantlets

Source :

English descriptors

Abstract

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