Nucleotide sequence analysis of the human KCNJ1 potassium channel locus
Identifieur interne : 003C54 ( Main/Exploration ); précédent : 003C53; suivant : 003C55Nucleotide sequence analysis of the human KCNJ1 potassium channel locus
Auteurs : Jeffrey H. Bock [États-Unis] ; Mary E. Shuck [États-Unis] ; Christopher W. Benjamin [États-Unis] ; Mea Chee [États-Unis] ; Michael J. Bienkowski [États-Unis] ; Jerry L. Slightom [États-Unis]Source :
- Gene [ 0378-1119 ] ; 1997.
English descriptors
- Teeft :
- Accession, Anking, Bock, Boim, Cdna, Clone, Direct sequencing, Distinct transcripts, Dna, Exon, Genbank, Genbank accession, Genomic, Hkcnj1, Hkcnj1 exon, Hkcnj1 exons, Hkcnj1 gene, Hkcnj1 locus, Hkcnj1 sequence, Human exon, Intron, Kcnj1, Kcnj1 locus, Locus, Multiple promoters, Nucleotide, Nucleotide positions, Nucleotide sequence, Orthologous, Polymerase, Primer, Primer extension reaction, Promoter, Promoter elements, Putative, Repetitive, Rkcnj1 gene, Sequence information, Sequencing, Sequencing strategy, Slightom, Template dnas, Transcript.
Abstract
Abstract: Detailed analyses of transcripts encoding various isoforms of the human potassium (K+, inward rectifying) channel ROM-K (also referred to as Kir1.1) revealed the existence of at least five distinct transcripts [Shuck et al., J. Biol. Chem. 269 (1994) 24261–24270]. These five hROM-K transcripts appear to be the result of alternative splicing of five exons. The nucleotide sequence of the genomic DNA including and spanning these exons (the KCNJ1 locus) was obtained directly from λ and P1 clones (a total of 40 kb). The organization of the hKCNJ1 gene was determined by combining this sequence information with data obtained from primer extension and RT-PCR experiments. It appears that the hKCNJ1 gene utilizes multiple promoters, with promoter-like elements found 5′ of exons 1, 4, or 5. The promoter 5′ of exon 5 was unexpected; thus, it appears that the hKCNJ1 gene is capable of producing six distinct hROM-K transcripts via the use of three promoters and alternative splicing of five exons. Comparisons of the rat and human ROM-K cDNA sequences find human homologs (orthologs) for two of the three distinct rROM-K transcripts. A search of the complete human KCNJ1 sequence with the exon sequence that defines the other rROM-K transcript located a region of shared nucleotides, a putative sixth exon, in the hKCNJ1 gene. This finding suggests that the rKCNJ1 gene may contain an exon that is no longer or infrequently used in transcripts derived from the hKCNJ1 gene.
Url:
DOI: 10.1016/S0378-1119(96)00759-7
Affiliations:
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Slightom</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Molecular Biology Unit 7242-267-510, Pharmacia and Upjohn Company, Kalamazoo, MI 49007</wicri:regionArea><wicri:noRegion>MI 49007</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">188</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="16">16</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Accession</term><term>Anking</term><term>Bock</term><term>Boim</term><term>Cdna</term><term>Clone</term><term>Direct sequencing</term><term>Distinct transcripts</term><term>Dna</term><term>Exon</term><term>Genbank</term><term>Genbank accession</term><term>Genomic</term><term>Hkcnj1</term><term>Hkcnj1 exon</term><term>Hkcnj1 exons</term><term>Hkcnj1 gene</term><term>Hkcnj1 locus</term><term>Hkcnj1 sequence</term><term>Human exon</term><term>Intron</term><term>Kcnj1</term><term>Kcnj1 locus</term><term>Locus</term><term>Multiple promoters</term><term>Nucleotide</term><term>Nucleotide positions</term><term>Nucleotide sequence</term><term>Orthologous</term><term>Polymerase</term><term>Primer</term><term>Primer extension reaction</term><term>Promoter</term><term>Promoter elements</term><term>Putative</term><term>Repetitive</term><term>Rkcnj1 gene</term><term>Sequence information</term><term>Sequencing</term><term>Sequencing strategy</term><term>Slightom</term><term>Template dnas</term><term>Transcript</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Detailed analyses of transcripts encoding various isoforms of the human potassium (K+, inward rectifying) channel ROM-K (also referred to as Kir1.1) revealed the existence of at least five distinct transcripts [Shuck et al., J. Biol. Chem. 269 (1994) 24261–24270]. These five hROM-K transcripts appear to be the result of alternative splicing of five exons. The nucleotide sequence of the genomic DNA including and spanning these exons (the KCNJ1 locus) was obtained directly from λ and P1 clones (a total of 40 kb). The organization of the hKCNJ1 gene was determined by combining this sequence information with data obtained from primer extension and RT-PCR experiments. It appears that the hKCNJ1 gene utilizes multiple promoters, with promoter-like elements found 5′ of exons 1, 4, or 5. The promoter 5′ of exon 5 was unexpected; thus, it appears that the hKCNJ1 gene is capable of producing six distinct hROM-K transcripts via the use of three promoters and alternative splicing of five exons. Comparisons of the rat and human ROM-K cDNA sequences find human homologs (orthologs) for two of the three distinct rROM-K transcripts. A search of the complete human KCNJ1 sequence with the exon sequence that defines the other rROM-K transcript located a region of shared nucleotides, a putative sixth exon, in the hKCNJ1 gene. This finding suggests that the rKCNJ1 gene may contain an exon that is no longer or infrequently used in transcripts derived from the hKCNJ1 gene.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Bock, Jeffrey H" sort="Bock, Jeffrey H" uniqKey="Bock J" first="Jeffrey H" last="Bock">Jeffrey H. Bock</name></noRegion><name sortKey="Benjamin, Christopher W" sort="Benjamin, Christopher W" uniqKey="Benjamin C" first="Christopher W" last="Benjamin">Christopher W. Benjamin</name><name sortKey="Bienkowski, Michael J" sort="Bienkowski, Michael J" uniqKey="Bienkowski M" first="Michael J" last="Bienkowski">Michael J. Bienkowski</name><name sortKey="Chee, Mea" sort="Chee, Mea" uniqKey="Chee M" first="Mea" last="Chee">Mea Chee</name><name sortKey="Shuck, Mary E" sort="Shuck, Mary E" uniqKey="Shuck M" first="Mary E" last="Shuck">Mary E. Shuck</name><name sortKey="Slightom, Jerry L" sort="Slightom, Jerry L" uniqKey="Slightom J" first="Jerry L" last="Slightom">Jerry L. Slightom</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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