Improved resolution in the detection of oligonucleotides up to 60-mers in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using pulsed-delayed extraction with a simple high voltage transistor switch.
Identifieur interne : 003C20 ( Main/Exploration ); précédent : 003C19; suivant : 003C21Improved resolution in the detection of oligonucleotides up to 60-mers in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using pulsed-delayed extraction with a simple high voltage transistor switch.
Auteurs : Y. Zhu [États-Unis] ; L. He ; J R Srinivasan ; D M LubmanSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 1997.
Descripteurs français
- KwdFr :
- MESH :
- analyse : ADN, Oligonucléotides.
- isolement et purification : Oligonucléotides, Spectrométrie de masse MALDI.
- Chromatographie en phase liquide à haute performance, Transistors électroniques.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : DNA, Oligonucleotides.
- chemical , isolation & purification : Oligonucleotides.
- instrumentation : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Chromatography, High Pressure Liquid, Transistors, Electronic.
Abstract
Pulsed-delayed extraction using a simple high voltage transistor switch together with various sample purification approaches were used to enhance the resolution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of oligonucleotides up to 60 bases long. This switch can provide a 0-3 kV voltage pulse with a 75 ns fall time. A resolution of 500-900 was typically observed for samples from 5-mers to 60-mers using pulsed-delayed extraction (PDE) with the switch described herein. The resolution deteriorated to < 100 for oligonucleotides of > or = 65-mers. With the TOF acceleration region configuration used in this work, the resolution was found not to vary significantly over a delay range of 2-5 mus. As the DNA size increased to over 35-mer, HPLC purification was required to retain the enhancement in resolution provided by PDE MALDI-MS.
DOI: 10.1002/(SICI)1097-0231(19970615)11:9<987::AID-RCM949>3.0.CO;2-J
PubMed: 9204573
Affiliations:
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Le document en format XML
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He</name></author><author><name sortKey="Srinivasan, J R" sort="Srinivasan, J R" uniqKey="Srinivasan J" first="J R" last="Srinivasan">J R Srinivasan</name></author><author><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1997">1997</date><idno type="RBID">pubmed:9204573</idno><idno type="pmid">9204573</idno><idno type="doi">10.1002/(SICI)1097-0231(19970615)11:9<987::AID-RCM949>3.0.CO;2-J</idno><idno type="wicri:Area/PubMed/Corpus">002742</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002742</idno><idno type="wicri:Area/PubMed/Curation">002742</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002742</idno><idno type="wicri:Area/PubMed/Checkpoint">002583</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002583</idno><idno type="wicri:Area/Ncbi/Merge">002A83</idno><idno type="wicri:Area/Ncbi/Curation">002A83</idno><idno type="wicri:Area/Ncbi/Checkpoint">002A83</idno><idno type="wicri:doubleKey">0951-4198:1997:Zhu Y:improved:resolution:in</idno><idno type="wicri:Area/Main/Merge">003C72</idno><idno type="wicri:Area/Main/Curation">003C20</idno><idno type="wicri:Area/Main/Exploration">003C20</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Improved resolution in the detection of oligonucleotides up to 60-mers in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using pulsed-delayed extraction with a simple high voltage transistor switch.</title><author><name sortKey="Zhu, Y" sort="Zhu, Y" uniqKey="Zhu Y" first="Y" last="Zhu">Y. Zhu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, University of Michigan, Ann Arbor 48109-1055, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Michigan, Ann Arbor 48109-1055</wicri:regionArea><wicri:noRegion>Ann Arbor 48109-1055</wicri:noRegion></affiliation></author><author><name sortKey="He, L" sort="He, L" uniqKey="He L" first="L" last="He">L. He</name></author><author><name sortKey="Srinivasan, J R" sort="Srinivasan, J R" uniqKey="Srinivasan J" first="J R" last="Srinivasan">J R Srinivasan</name></author><author><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name></author></analytic><series><title level="j">Rapid communications in mass spectrometry : RCM</title><idno type="ISSN">0951-4198</idno><imprint><date when="1997" type="published">1997</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>DNA (analysis)</term><term>Oligonucleotides (analysis)</term><term>Oligonucleotides (isolation & purification)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation)</term><term>Transistors, Electronic</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term><term>Chromatographie en phase liquide à haute performance</term><term>Oligonucléotides (analyse)</term><term>Oligonucléotides (isolement et purification)</term><term>Spectrométrie de masse MALDI (instrumentation)</term><term>Transistors électroniques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term><term>Oligonucleotides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Oligonucleotides</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term><term>Oligonucléotides</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Oligonucléotides</term><term>Spectrométrie de masse MALDI</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Transistors, Electronic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Transistors électroniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Pulsed-delayed extraction using a simple high voltage transistor switch together with various sample purification approaches were used to enhance the resolution in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) of oligonucleotides up to 60 bases long. This switch can provide a 0-3 kV voltage pulse with a 75 ns fall time. A resolution of 500-900 was typically observed for samples from 5-mers to 60-mers using pulsed-delayed extraction (PDE) with the switch described herein. The resolution deteriorated to < 100 for oligonucleotides of > or = 65-mers. With the TOF acceleration region configuration used in this work, the resolution was found not to vary significantly over a delay range of 2-5 mus. As the DNA size increased to over 35-mer, HPLC purification was required to retain the enhancement in resolution provided by PDE MALDI-MS.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="He, L" sort="He, L" uniqKey="He L" first="L" last="He">L. He</name><name sortKey="Lubman, D M" sort="Lubman, D M" uniqKey="Lubman D" first="D M" last="Lubman">D M Lubman</name><name sortKey="Srinivasan, J R" sort="Srinivasan, J R" uniqKey="Srinivasan J" first="J R" last="Srinivasan">J R Srinivasan</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Zhu, Y" sort="Zhu, Y" uniqKey="Zhu Y" first="Y" last="Zhu">Y. Zhu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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