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Altered expression of mitochondrial 16S ribosomal RNA in p53-deficient mouse embryos revealed by differential display

Identifieur interne : 003A99 ( Main/Exploration ); précédent : 003A98; suivant : 003B00

Altered expression of mitochondrial 16S ribosomal RNA in p53-deficient mouse embryos revealed by differential display

Auteurs : Monier M. Ibrahim [États-Unis] ; Marjaneh Razmara [États-Unis] ; Diep Nguyen [États-Unis] ; Ronald J. Donahue [États-Unis] ; Judith A. Wubah [États-Unis] ; Thomas B. Knudsen [États-Unis]

Source :

BBA - Molecular Cell Research [ 0167-4889 ] ; 1998.

RBID : ISTEX:F2203F877DB9F286BD7D55DE90D091AF8EC1144A

English descriptors

Teeft :
- Acta, Anterior ntds, Apoptosis, Bbamcr, Biochimica, Biol, Biophysica, Biophysica acta, Cell biol, Convex nacl gradient, Cytochrome, Developmental expression, Display peaks, Display pools, Display products, Dntp concentration, Early presomite stage, Elsevier science, Embryo, Ethidium bromide, Genbank accession number, Genome, Genotype, Hplc, Hplc column, Identical patterns, Likely basis, Metabolism, Mitochondrial, Mitochondrial mrnas, Mitochondrial translation, Mobile phase, Mouse embryos, Mouse mitochondrial genome, Mrna, Mrna display, Mtdna, Mtdna genome, Nacl gradient, National institute, Nature genet, Negative controls, Neurulation, Neurulation stage embryos, Neurulation stage mouse embryos, Nucleotide positions, Oxidase, Oxidase staining, Oxidase subunit, Oxidative, Oxidative metabolism, Photodiode array detection, Post coitum, Present study, Primer, Primer pairs, Protein activity, Respiratory chain, Respiring cells, Rrna, Rrna expression, Rrna levels, Rrna transcripts, Sample dilutions, Small subset, Somite, Somite pair stage, Somite pairs, Subunit, Transcript, Tumor suppressor, Waters associates.

Abstract

Abstract: Inactivation of the tumor suppressor p53 is associated with neural tube defects and altered teratogenicity in early embryos. To gain insight into the function of p53 during early embryogenesis, RNA profiles of wild-type p53(+/+) and p53(−/−) null mutant mouse embryos were compared at the head-fold stage (day 8 post coitum) using HPLC-based mRNA differential display. The results of this screen revealed a deficiency of mitochondrial 16S ribosomal RNA in p53(−/−) embryos. RT-PCR showed abnormalities in 16S rRNA levels relative to some representative nuclear (COIV, β-actin) and mitochondrial (COIII) transcripts in p53(−/−) embryos, and that 16S rRNA expression increased with development of p53(+/+) embryos during neurulation. Embryos that lack p53 also displayed weakened cytochrome c oxidase staining and reduced ATP content. During neurulation, the mouse embryo switches from an anaerobic (glycolytic) to an aerobic (oxidative) metabolism. The preliminary results of the present study suggest that p53 may be involved, directly or indirectly, in this transition.

Url:

https://api.istex.fr/ark:/67375/6H6-MBWKBMZS-8/fulltext.pdf

DOI: 10.1016/S0167-4889(98)00066-4

Affiliations:

États-Unis

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Le document en format XML

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<term>Biophysica</term>
<term>Biophysica acta</term>
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<term>Convex nacl gradient</term>
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<front><div type="abstract" xml:lang="en">Abstract: Inactivation of the tumor suppressor p53 is associated with neural tube defects and altered teratogenicity in early embryos. To gain insight into the function of p53 during early embryogenesis, RNA profiles of wild-type p53(+/+) and p53(−/−) null mutant mouse embryos were compared at the head-fold stage (day 8 post coitum) using HPLC-based mRNA differential display. The results of this screen revealed a deficiency of mitochondrial 16S ribosomal RNA in p53(−/−) embryos. RT-PCR showed abnormalities in 16S rRNA levels relative to some representative nuclear (COIV, β-actin) and mitochondrial (COIII) transcripts in p53(−/−) embryos, and that 16S rRNA expression increased with development of p53(+/+) embryos during neurulation. Embryos that lack p53 also displayed weakened cytochrome c oxidase staining and reduced ATP content. During neurulation, the mouse embryo switches from an anaerobic (glycolytic) to an aerobic (oxidative) metabolism. The preliminary results of the present study suggest that p53 may be involved, directly or indirectly, in this transition.</div>
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Altered expression of mitochondrial 16S ribosomal RNA in p53-deficient mouse embryos revealed by differential display

Altered expression of mitochondrial 16S ribosomal RNA in p53-deficient mouse embryos revealed by differential display

Source :

English descriptors

Abstract

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Le document en format XML

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