Cyclobutane thymine dimers with a disrupted phosphodiester bond are refractory to T4 endonuclease V digestion but have increased sensitivity to UvrABC nuclease.
Identifieur interne : 003A12 ( Main/Exploration ); précédent : 003A11; suivant : 003A13Cyclobutane thymine dimers with a disrupted phosphodiester bond are refractory to T4 endonuclease V digestion but have increased sensitivity to UvrABC nuclease.
Auteurs : Y. Zheng [États-Unis] ; D. Hunting ; M S TangSource :
- Biochemistry [ 0006-2960 ] ; 1998.
Descripteurs français
- KwdFr :
- ADN (), ADN (effets des radiations), Altération de l'ADN, Animaux, Bovins, Conformation d'acide nucléique, Deoxyribonuclease (pyrimidine dimer), Dimères de pyrimidine (), Dimères de pyrimidine (métabolisme), Dimérisation, Endodeoxyribonucleases (métabolisme), Escherichia coli (enzymologie), Ligases (métabolisme), Oligodésoxyribonucléotides (), Oligodésoxyribonucléotides (effets des radiations), Oligodésoxyribonucléotides (métabolisme), Protéines Escherichia coli, Protéines virales, Rayons ultraviolets, Réparation de l'ADN, Séquence nucléotidique, Techniques in vitro.
- MESH :
- effets des radiations : ADN, Oligodésoxyribonucléotides.
- enzymologie : Escherichia coli.
- métabolisme : Dimères de pyrimidine, Endodeoxyribonucleases, Ligases, Oligodésoxyribonucléotides.
- ADN, Altération de l'ADN, Animaux, Bovins, Conformation d'acide nucléique, Deoxyribonuclease (pyrimidine dimer), Dimères de pyrimidine, Dimérisation, Oligodésoxyribonucléotides, Protéines Escherichia coli, Protéines virales, Rayons ultraviolets, Réparation de l'ADN, Séquence nucléotidique, Techniques in vitro.
English descriptors
- KwdEn :
- Animals, Base Sequence, Cattle, DNA (chemistry), DNA (radiation effects), DNA Damage, DNA Repair, Deoxyribonuclease (Pyrimidine Dimer), Dimerization, Endodeoxyribonucleases (metabolism), Escherichia coli (enzymology), Escherichia coli Proteins, In Vitro Techniques, Ligases (metabolism), Nucleic Acid Conformation, Oligodeoxyribonucleotides (chemistry), Oligodeoxyribonucleotides (metabolism), Oligodeoxyribonucleotides (radiation effects), Pyrimidine Dimers (chemistry), Pyrimidine Dimers (metabolism), Ultraviolet Rays, Viral Proteins.
- MESH :
- chemical , chemistry : DNA, Oligodeoxyribonucleotides, Pyrimidine Dimers.
- chemical , metabolism : Endodeoxyribonucleases, Ligases, Oligodeoxyribonucleotides, Pyrimidine Dimers.
- chemical , radiation effects : DNA, Oligodeoxyribonucleotides.
- enzymology : Escherichia coli.
- Animals, Base Sequence, Cattle, DNA Damage, DNA Repair, Deoxyribonuclease (Pyrimidine Dimer), Dimerization, Escherichia coli Proteins, In Vitro Techniques, Nucleic Acid Conformation, Ultraviolet Rays, Viral Proteins.
Abstract
UV irradiation induces the dimerization of synthetic single-stranded, 80-mer oligonucleotides with self-complementary, alternating purine-pyrimidine sequences, and terminal 5'- and 3'-thymines; this process can be reversed by photoreactivation. The UV-induced 160-mers are sensitive to digestion by the restriction enzyme SnaBI, but monomers are insensitive to digestion, indicating that UV irradiation stabilizes the formation of double-stranded DNA. These results suggest that UV irradiation of these 80-mer oligonucleotide substrates induces the formation of a novel cyclobutane thymine dimer which lacks an intradimer phosphodiester bond (CPD*). This CPD*, linking the terminal thymines of two separate 80-mer molecules, is formed in a double-stranded DNA region created by self-annealing and intermolecular hybridization of the two 80-mer strands. We have found that these UV-induced CPD* in 160-mers are sensitive to cleavage by the nucleotide excision enzyme complex UvrABC nuclease, but resistant to cleavage by the cyclobutane pyrimidine dimer-specific enzyme T4 endonuclease V. However, pretreatment of the 160-mers with ligase reverses their sensitivity to these two enzymes, significantly reducing their susceptibility to cleavage by UvrABC nuclease but dramatically increasing their susceptibility to cleavage by T4 endonuclease. The biological significance of these findings is discussed.
DOI: 10.1021/bi972523y
PubMed: 9521643
Affiliations:
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Zheng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville, Texas 78957</wicri:regionArea><wicri:noRegion>Texas 78957</wicri:noRegion></affiliation></author><author><name sortKey="Hunting, D" sort="Hunting, D" uniqKey="Hunting D" first="D" last="Hunting">D. Hunting</name></author><author><name sortKey="Tang, M S" sort="Tang, M S" uniqKey="Tang M" first="M S" last="Tang">M S Tang</name></author></analytic><series><title level="j">Biochemistry</title><idno type="ISSN">0006-2960</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cattle</term><term>DNA (chemistry)</term><term>DNA (radiation effects)</term><term>DNA Damage</term><term>DNA Repair</term><term>Deoxyribonuclease (Pyrimidine Dimer)</term><term>Dimerization</term><term>Endodeoxyribonucleases (metabolism)</term><term>Escherichia coli (enzymology)</term><term>Escherichia coli Proteins</term><term>In Vitro Techniques</term><term>Ligases (metabolism)</term><term>Nucleic Acid Conformation</term><term>Oligodeoxyribonucleotides (chemistry)</term><term>Oligodeoxyribonucleotides (metabolism)</term><term>Oligodeoxyribonucleotides (radiation effects)</term><term>Pyrimidine Dimers (chemistry)</term><term>Pyrimidine Dimers (metabolism)</term><term>Ultraviolet Rays</term><term>Viral Proteins</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>ADN (effets des radiations)</term><term>Altération de l'ADN</term><term>Animaux</term><term>Bovins</term><term>Conformation d'acide nucléique</term><term>Deoxyribonuclease (pyrimidine dimer)</term><term>Dimères de pyrimidine ()</term><term>Dimères de pyrimidine (métabolisme)</term><term>Dimérisation</term><term>Endodeoxyribonucleases (métabolisme)</term><term>Escherichia coli (enzymologie)</term><term>Ligases (métabolisme)</term><term>Oligodésoxyribonucléotides ()</term><term>Oligodésoxyribonucléotides (effets des radiations)</term><term>Oligodésoxyribonucléotides (métabolisme)</term><term>Protéines Escherichia coli</term><term>Protéines virales</term><term>Rayons ultraviolets</term><term>Réparation de l'ADN</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA</term><term>Oligodeoxyribonucleotides</term><term>Pyrimidine Dimers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Endodeoxyribonucleases</term><term>Ligases</term><term>Oligodeoxyribonucleotides</term><term>Pyrimidine Dimers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="radiation effects" xml:lang="en"><term>DNA</term><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="effets des radiations" xml:lang="fr"><term>ADN</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dimères de pyrimidine</term><term>Endodeoxyribonucleases</term><term>Ligases</term><term>Oligodésoxyribonucléotides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Base Sequence</term><term>Cattle</term><term>DNA Damage</term><term>DNA Repair</term><term>Deoxyribonuclease (Pyrimidine Dimer)</term><term>Dimerization</term><term>Escherichia coli Proteins</term><term>In Vitro Techniques</term><term>Nucleic Acid Conformation</term><term>Ultraviolet Rays</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Altération de l'ADN</term><term>Animaux</term><term>Bovins</term><term>Conformation d'acide nucléique</term><term>Deoxyribonuclease (pyrimidine dimer)</term><term>Dimères de pyrimidine</term><term>Dimérisation</term><term>Oligodésoxyribonucléotides</term><term>Protéines Escherichia coli</term><term>Protéines virales</term><term>Rayons ultraviolets</term><term>Réparation de l'ADN</term><term>Séquence nucléotidique</term><term>Techniques in vitro</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">UV irradiation induces the dimerization of synthetic single-stranded, 80-mer oligonucleotides with self-complementary, alternating purine-pyrimidine sequences, and terminal 5'- and 3'-thymines; this process can be reversed by photoreactivation. The UV-induced 160-mers are sensitive to digestion by the restriction enzyme SnaBI, but monomers are insensitive to digestion, indicating that UV irradiation stabilizes the formation of double-stranded DNA. These results suggest that UV irradiation of these 80-mer oligonucleotide substrates induces the formation of a novel cyclobutane thymine dimer which lacks an intradimer phosphodiester bond (CPD*). This CPD*, linking the terminal thymines of two separate 80-mer molecules, is formed in a double-stranded DNA region created by self-annealing and intermolecular hybridization of the two 80-mer strands. We have found that these UV-induced CPD* in 160-mers are sensitive to cleavage by the nucleotide excision enzyme complex UvrABC nuclease, but resistant to cleavage by the cyclobutane pyrimidine dimer-specific enzyme T4 endonuclease V. However, pretreatment of the 160-mers with ligase reverses their sensitivity to these two enzymes, significantly reducing their susceptibility to cleavage by UvrABC nuclease but dramatically increasing their susceptibility to cleavage by T4 endonuclease. The biological significance of these findings is discussed.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Hunting, D" sort="Hunting, D" uniqKey="Hunting D" first="D" last="Hunting">D. Hunting</name><name sortKey="Tang, M S" sort="Tang, M S" uniqKey="Tang M" first="M S" last="Tang">M S Tang</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Zheng, Y" sort="Zheng, Y" uniqKey="Zheng Y" first="Y" last="Zheng">Y. Zheng</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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