MerMade: an oligodeoxyribonucleotide synthesizer for high throughput oligonucleotide production in dual 96-well plates.
Identifieur interne : 003993 ( Main/Exploration ); précédent : 003992; suivant : 003994MerMade: an oligodeoxyribonucleotide synthesizer for high throughput oligonucleotide production in dual 96-well plates.
Auteurs : S. Rayner [États-Unis] ; S. Brignac ; R. Bumeister ; Y. Belosludtsev ; T. Ward ; O. Grant ; K. O'Brien ; G A Evans ; H R GarnerSource :
- Genome research [ 1088-9051 ] ; 1998.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN (), Analyse de séquence d'ADN (instrumentation), Cartographie chromosomique (), Cartographie chromosomique (instrumentation), Cartographie de contigs (), Cartographie de contigs (instrumentation), Cosmides, Logiciel, Oligodésoxyribonucléotides (synthèse chimique), Réaction de polymérisation en chaîne (), Réaction de polymérisation en chaîne (instrumentation).
- MESH :
- synthèse chimique : Analyse de séquence d'ADN, Cartographie chromosomique, Cartographie de contigs, Oligodésoxyribonucléotides, Réaction de polymérisation en chaîne.
- Analyse de séquence d'ADN, Cartographie chromosomique, Cartographie de contigs, Cosmides, Logiciel, Réaction de polymérisation en chaîne.
English descriptors
- KwdEn :
- Chromosome Mapping (instrumentation), Chromosome Mapping (methods), Contig Mapping (instrumentation), Contig Mapping (methods), Cosmids, Oligodeoxyribonucleotides (chemical synthesis), Polymerase Chain Reaction (instrumentation), Polymerase Chain Reaction (methods), Sequence Analysis, DNA (instrumentation), Sequence Analysis, DNA (methods), Software.
- MESH :
- chemical , chemical synthesis : Oligodeoxyribonucleotides.
- instrumentation : Chromosome Mapping, Contig Mapping, Polymerase Chain Reaction, Sequence Analysis, DNA.
- methods : Chromosome Mapping, Contig Mapping, Polymerase Chain Reaction, Sequence Analysis, DNA.
- Cosmids, Software.
Abstract
We have designed and constructed a machine that synthesizes two standard 96-well plates of oligonucleotides in a single run using standard phosphoramidite chemistry. The machine is capable of making a combination of standard, degenerate, or modified oligos in a single plate. The run time is typically 17 hr for two plates of 20-mers and a reaction scale of 40 nM. The reaction vessel is a standard polypropylene 96-well plate with a hole drilled in the bottom of each well. The two plates are placed in separate vacuum chucks and mounted on an xy table. Each well in turn is positioned under the appropriate reagent injection line and the reagent is injected by switching a dedicated valve. All aspects of machine operation are controlled by a Macintosh computer, which also guides the user through the startup and shutdown procedures, provides a continuous update on the status of the run, and facilitates a number of service procedures that need to be carried out periodically. Over 25,000 oligos have been synthesized for use in dye terminator sequencing reactions, polymerase chain reactions (PCRs), hybridization, and RT-PCR. Oligos up to 100 bases in length have been made with a coupling efficiency in excess of 99%. These machines, working in conjunction with our oligo prediction code are particularly well suited to application in automated high throughput genomic sequencing.
DOI: 10.1101/gr.8.7.741
PubMed: 9685322
Affiliations:
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Le document en format XML
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Rayner</name><affiliation wicri:level="2"><nlm:affiliation>Department of Internal Medicine, The University of Texas Southwestern (UTSW) Medical Center at Dallas, Dallas, Texas 75235-8591 USA. rayner@ryburn.swmed.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Texas</region></placeName><wicri:cityArea>Department of Internal Medicine, The University of Texas Southwestern (UTSW) Medical Center at Dallas, Dallas</wicri:cityArea></affiliation></author><author><name sortKey="Brignac, S" sort="Brignac, S" uniqKey="Brignac S" first="S" last="Brignac">S. Brignac</name></author><author><name sortKey="Bumeister, R" sort="Bumeister, R" uniqKey="Bumeister R" first="R" last="Bumeister">R. Bumeister</name></author><author><name sortKey="Belosludtsev, Y" sort="Belosludtsev, Y" uniqKey="Belosludtsev Y" first="Y" last="Belosludtsev">Y. Belosludtsev</name></author><author><name sortKey="Ward, T" sort="Ward, T" uniqKey="Ward T" first="T" last="Ward">T. Ward</name></author><author><name sortKey="Grant, O" sort="Grant, O" uniqKey="Grant O" first="O" last="Grant">O. Grant</name></author><author><name sortKey="O Brien, K" sort="O Brien, K" uniqKey="O Brien K" first="K" last="O'Brien">K. O'Brien</name></author><author><name sortKey="Evans, G A" sort="Evans, G A" uniqKey="Evans G" first="G A" last="Evans">G A Evans</name></author><author><name sortKey="Garner, H R" sort="Garner, H R" uniqKey="Garner H" first="H R" last="Garner">H R Garner</name></author></analytic><series><title level="j">Genome research</title><idno type="ISSN">1088-9051</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chromosome Mapping (instrumentation)</term><term>Chromosome Mapping (methods)</term><term>Contig Mapping (instrumentation)</term><term>Contig Mapping (methods)</term><term>Cosmids</term><term>Oligodeoxyribonucleotides (chemical synthesis)</term><term>Polymerase Chain Reaction (instrumentation)</term><term>Polymerase Chain Reaction (methods)</term><term>Sequence Analysis, DNA (instrumentation)</term><term>Sequence Analysis, DNA (methods)</term><term>Software</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN ()</term><term>Analyse de séquence d'ADN (instrumentation)</term><term>Cartographie chromosomique ()</term><term>Cartographie chromosomique (instrumentation)</term><term>Cartographie de contigs ()</term><term>Cartographie de contigs (instrumentation)</term><term>Cosmides</term><term>Logiciel</term><term>Oligodésoxyribonucléotides (synthèse chimique)</term><term>Réaction de polymérisation en chaîne ()</term><term>Réaction de polymérisation en chaîne (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemical synthesis" xml:lang="en"><term>Oligodeoxyribonucleotides</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Chromosome Mapping</term><term>Contig Mapping</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromosome Mapping</term><term>Contig Mapping</term><term>Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie chromosomique</term><term>Cartographie de contigs</term><term>Oligodésoxyribonucléotides</term><term>Réaction de polymérisation en chaîne</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cosmids</term><term>Software</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie chromosomique</term><term>Cartographie de contigs</term><term>Cosmides</term><term>Logiciel</term><term>Réaction de polymérisation en chaîne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have designed and constructed a machine that synthesizes two standard 96-well plates of oligonucleotides in a single run using standard phosphoramidite chemistry. 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Oligos up to 100 bases in length have been made with a coupling efficiency in excess of 99%. These machines, working in conjunction with our oligo prediction code are particularly well suited to application in automated high throughput genomic sequencing.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Texas</li></region></list><tree><noCountry><name sortKey="Belosludtsev, Y" sort="Belosludtsev, Y" uniqKey="Belosludtsev Y" first="Y" last="Belosludtsev">Y. Belosludtsev</name><name sortKey="Brignac, S" sort="Brignac, S" uniqKey="Brignac S" first="S" last="Brignac">S. Brignac</name><name sortKey="Bumeister, R" sort="Bumeister, R" uniqKey="Bumeister R" first="R" last="Bumeister">R. Bumeister</name><name sortKey="Evans, G A" sort="Evans, G A" uniqKey="Evans G" first="G A" last="Evans">G A Evans</name><name sortKey="Garner, H R" sort="Garner, H R" uniqKey="Garner H" first="H R" last="Garner">H R Garner</name><name sortKey="Grant, O" sort="Grant, O" uniqKey="Grant O" first="O" last="Grant">O. Grant</name><name sortKey="O Brien, K" sort="O Brien, K" uniqKey="O Brien K" first="K" last="O'Brien">K. O'Brien</name><name sortKey="Ward, T" sort="Ward, T" uniqKey="Ward T" first="T" last="Ward">T. Ward</name></noCountry><country name="États-Unis"><region name="Texas"><name sortKey="Rayner, S" sort="Rayner, S" uniqKey="Rayner S" first="S" last="Rayner">S. Rayner</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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