ADHESION OF L1210 CELLS TO SULFONATED STYRENE COPOLYMER SURFACES: IMAGING OF F-ACTIN AND α-ACTININ
Identifieur interne : 003971 ( Main/Exploration ); précédent : 003970; suivant : 003972ADHESION OF L1210 CELLS TO SULFONATED STYRENE COPOLYMER SURFACES: IMAGING OF F-ACTIN AND α-ACTININ
Auteurs : H. M Kowalczy Ska [Pologne] ; M. Nowak-Wyrzykowska [Pologne]Source :
- Cell Biology International [ 1065-6995 ] ; 1999.
English descriptors
- KwdEn :
- Teeft :
- Actin, Actin cytoskeleton, Actinin, Actinin distribution, Adherent, Adherent cells, Adhesion, Biol, Bronectin, Cell, Cell adhesion, Cell biol, Cell biology, Cell detachment, Cell image analysis, Cell regions, Central part, Copolymer, Copolymer surface, Copolymer surfaces, Curr opin cell biol, Cytoskeleton, Distinctness, Entire population, Extracellular matrix, Focus plane, High sulfonic group density, Integrins, Interaction time, Kaminski, Kowalczynska, Microvilli, Optical slice, Optical slices, Previous results, Receptor, Relative number, Sedimented cells, Serumcontaining medium, Shearing force, Small dots, So3h, So3h groups, Static adhesion, Static conditions, Strong distinctness, Styrene content, Styrene copolymer surfaces, Styrene mers, Substrata, Substratum, Substratum surface, Sulfonated, Sulfonated copolymer surfaces, Sulfonated styrene copolymer surfaces, Sulfonated surfaces, Sulfonic, Sulfonic group content, Sulfonic groups, Surface content, Surface density, Surface sulfonic groups, Weak distinctness.
Abstract
Abstract: The static adhesion of living L1210 cells to sulfonated copolymer surfaces of different sulfonic group content and the actin cytoskeleton organization in the adhering cells were studied. The strength of the cell–substratum interaction was estimated by determining the relative number of cells remaining adherent despite experiencing a shearing force equal to 1.25×10−11N caused by the laminar flow of the medium. The cell–substratum interaction took place in a medium with or without serum. The distribution of F-actin and α-actinin in the adhering cells was determined in sequences of fluorescent images of cell optical slices with the use of a computer method of cell image analysis. It was shown that the surface sulfonic groups affect not only the rate and strength of cell–substratum adhesion but also the F-actin and α-actinin distribution (in the cell regions near the substratum surface) in cells adhering in the medium containing serum. These proteins, concentrated in the tips of microvilli, were observed as dots. The distinctness (discernibleness) and sizes of these dots depend on the surface content of sulfonic groups. F-actin is located at the periphery of the cells in cells adhering in the medium without serum and α-actinin is concentrated in small dots at the periphery and in the central part of the cells.
Url:
- https://api.istex.fr/ark:/67375/6H6-XG9LWVC7-N/fulltext.pdf
- https://api.istex.fr/ark:/67375/WNG-0S5V06D0-K/fulltext.pdf
DOI: 10.1006/cbir.1999.0358
Affiliations:
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M" last="Kowalczy Ska">H. M Kowalczy Ska</name><affiliation wicri:level="1"><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Biophysics and Biomathematics, Medical Centre for Postgraduate Education, ul. Marymoncka 99, 01-813, Warszawa</wicri:regionArea><wicri:noRegion>Warszawa</wicri:noRegion></affiliation></author><author><name sortKey="Nowak Wyrzykowska, M" sort="Nowak Wyrzykowska, M" uniqKey="Nowak Wyrzykowska M" first="M" last="Nowak-Wyrzykowska">M. Nowak-Wyrzykowska</name><affiliation wicri:level="1"><country xml:lang="fr">Pologne</country><wicri:regionArea>Department of Biophysics and Biomathematics, Medical Centre for Postgraduate Education, ul. Marymoncka 99, 01-813, Warszawa</wicri:regionArea><wicri:noRegion>Warszawa</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell Biology International</title><title level="j" type="abbrev">YCBIR</title><idno type="ISSN">1065-6995</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1999">1999</date><biblScope unit="volume">23</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="359">359</biblScope><biblScope unit="page" to="372">372</biblScope></imprint><idno type="ISSN">1065-6995</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1065-6995</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>F-actin and α-actinin imaging</term><term>cell adhesion</term><term>surface sulfonic groups</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Actin</term><term>Actin cytoskeleton</term><term>Actinin</term><term>Actinin distribution</term><term>Adherent</term><term>Adherent cells</term><term>Adhesion</term><term>Biol</term><term>Bronectin</term><term>Cell</term><term>Cell adhesion</term><term>Cell biol</term><term>Cell biology</term><term>Cell detachment</term><term>Cell image analysis</term><term>Cell regions</term><term>Central part</term><term>Copolymer</term><term>Copolymer surface</term><term>Copolymer surfaces</term><term>Curr opin cell biol</term><term>Cytoskeleton</term><term>Distinctness</term><term>Entire population</term><term>Extracellular matrix</term><term>Focus plane</term><term>High sulfonic group density</term><term>Integrins</term><term>Interaction time</term><term>Kaminski</term><term>Kowalczynska</term><term>Microvilli</term><term>Optical slice</term><term>Optical slices</term><term>Previous results</term><term>Receptor</term><term>Relative number</term><term>Sedimented cells</term><term>Serumcontaining medium</term><term>Shearing force</term><term>Small dots</term><term>So3h</term><term>So3h groups</term><term>Static adhesion</term><term>Static conditions</term><term>Strong distinctness</term><term>Styrene content</term><term>Styrene copolymer surfaces</term><term>Styrene mers</term><term>Substrata</term><term>Substratum</term><term>Substratum surface</term><term>Sulfonated</term><term>Sulfonated copolymer surfaces</term><term>Sulfonated styrene copolymer surfaces</term><term>Sulfonated surfaces</term><term>Sulfonic</term><term>Sulfonic group content</term><term>Sulfonic groups</term><term>Surface content</term><term>Surface density</term><term>Surface sulfonic groups</term><term>Weak distinctness</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The static adhesion of living L1210 cells to sulfonated copolymer surfaces of different sulfonic group content and the actin cytoskeleton organization in the adhering cells were studied. The strength of the cell–substratum interaction was estimated by determining the relative number of cells remaining adherent despite experiencing a shearing force equal to 1.25×10−11N caused by the laminar flow of the medium. The cell–substratum interaction took place in a medium with or without serum. The distribution of F-actin and α-actinin in the adhering cells was determined in sequences of fluorescent images of cell optical slices with the use of a computer method of cell image analysis. It was shown that the surface sulfonic groups affect not only the rate and strength of cell–substratum adhesion but also the F-actin and α-actinin distribution (in the cell regions near the substratum surface) in cells adhering in the medium containing serum. These proteins, concentrated in the tips of microvilli, were observed as dots. The distinctness (discernibleness) and sizes of these dots depend on the surface content of sulfonic groups. F-actin is located at the periphery of the cells in cells adhering in the medium without serum and α-actinin is concentrated in small dots at the periphery and in the central part of the cells.</div></front></TEI><affiliations><list><country><li>Pologne</li></country></list><tree><country name="Pologne"><noRegion><name sortKey="Kowalczy Ska, H M" sort="Kowalczy Ska, H M" uniqKey="Kowalczy Ska H" first="H. M" last="Kowalczy Ska">H. M Kowalczy Ska</name></noRegion><name sortKey="Nowak Wyrzykowska, M" sort="Nowak Wyrzykowska, M" uniqKey="Nowak Wyrzykowska M" first="M" last="Nowak-Wyrzykowska">M. Nowak-Wyrzykowska</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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